Cell Cycle, Mitosis and Meiosis

The cell cycle multiplies cells

 The cell cycle is an ordered sequence of events

that extends
– from the time a cell is first formed from a dividing
parent cell
– until its own division.

© 2012 Pearson Education, Inc.

Cell division is a continuum of dynamic
changes
 During cytokinesis, the cytoplasm is divided into separate
cells.
 The process of cytokinesis differs in animal and plant cells.
Cytokinesis
Cleavage
furrow Contracting ring of
microfilaments

Daughter
cells

Cleavage
furrow
 Anchorage, cell density, and chemical growth
factors affect cell division
 The cells within an organism’s body divide and
develop at different rates.
 Cell division is influenced externally by
– the presence of essential nutrients,
– growth factors, proteins that stimulate division, there are
over 50 different growth factors which work for one or
more cell type
– density-dependent inhibition, in which crowded cells
stop dividing,
– anchorage dependence, the need for cells to be in
contact with a solid surface to divide.
 Growth factors signal the cell cycle control
system
 The cell cycle control system is a cycling set of
molecules in the cell that
– triggers and
– coordinates key events in the cell cycle.

 Checkpoints in the cell cycle can

– stop an event or
– signal an event to proceed.
 Growth factors signal the cell cycle control
system
 There are three major checkpoints in the cell cycle.

G1- commitment to divide, growth

factors present?, Size of cell ok?,

S Phase – Genetic Material

Duplicates

G2- check for proper DNA replication

M- all chromosomes attached to

spindle fibers
 Cell Cycle progresses by action of Cdks

Cyclins
proteins produced by the cell during cell division

Cyclin-dependent kinases (Cdk)

cyclin is required to activate these enzymes
activates cell proteins by phosphorylating them
(proteins needed for S phase)
needed to go through G1 checkpoint

MPF
Maturation-promoting factor (mitosis promoting factor)
aka Mitosis- promoting factor is a cyclin-Cdk complex
phosphorylates proteins needed for mitosis
needed to go through G2 checkpoint
 Rate of Cell Division
• Differs from one cell type to the next
– Examples:
• red bone marrow cells divide every 12 hours to replace RBCs that
wear out
• Cells at tip of root divide about every 19 hours.
• Neurons (nerve cells) normally never divide again once brain is fully
formed in utero

• Control of Division, lost = CANCER

– Cancer is different depending on the tissue affected
– Common theme is lack of control over cell division
– Abnormal, uncontrolled cell division
– Mutation in genes (including p53) that target and control
abnormal cells.
– Abnormal cells impede functioning of normal cells
 p53 gene ( tumor suppressor gene)
• Key role in G1 checkpoint
• P53 protein monitors DNA
• Found absent or damaged in most cancer cells
 Cancer is failure of
cell cycle control
• Tumor suppressor genes- prevents the
development of mutated cells, prevents
cancer/tumors

• Oncogenes- cancer causing genes

• Proto-oncogenes- normal genes that

become mutated
 Meiosis
• Production/formation of __________

• Basis of sexual reproduction

• Only germ cells undergo meiosis
 Haploid gametes (n  23)

Egg cell
n
Sperm cell
Meiosis Fertilization

Ovary Testis

Diploid
zygote 2n
(2n  46)

Key
Mitosis Haploid stage (n)
Multicellular diploid
adults (2n  46) Diploid stage (2n)
 How meiosis halves chromosome number…

INTERPHASE MEIOSIS I MEIOSIS II

Sister
chromatids

1 2 3

A pair of A pair of
homologous duplicated
chromosomes homologous
in a diploid chromosomes
parent cell
 MEIOSIS I: Homologous chromosomes separate
INTERPHASE:
Chromosomes duplicate Prophase I Metaphase I Anaphase I

Centrosomes Spindle microtubules Sister chromatids

(with centriole Sites of crossing over
attached to a kinetochore remain attached
pairs) Centrioles Spindle

Tetrad
Nuclear Chromatin Sister Metaphase
Centromere
envelope chromatids Fragments (with a plate Homologous
of the kinetochore) chromosomes
nuclear separate
envelope
 MEIOSIS II: Sister chromatids separate
Telophase II
Prophase II Metaphase II Anaphase II and Cytokinesis

Sister chromatids Haploid daughter

separate cells forming
 Meiosis Leads to Genetic Diversity
 Three ways genetic diversity is increased by
meiosis:
1. 2 parents contribute ½ of the genetic material to offspring
2. Crossing-over in Prophase I
3. Chromosome Alignment in Metaphase I

 Meiosis produces cells that are NOT identical,

unique gametes
Summary of Cell Cycle

 1)Interphase ( G1 Phase, S Phase, G2 Phase)

 Cell duplicates membranes, ribosomes, organelles and DNA
 Terminates when mitosis begins.
2) Cell Division
 Meiosis is a form of cell division that forms sex cells
 Mitosis is the division and distribution of genetic material to new cells.
 Stages of Mitosis: prophase, metaphase, anaphase and telophase
3) Cytoplamic Division
 Distributes cytoplasm into two portions completing about the same time as mitosis.
.
4) Differentiation is the process of cell specialization.
 Process of cell specialization
 Stem Cells provide new cells for growth ad repair. They give rise to other
stem cells ad to progenitor cells that begin to specialize.
 Reflects the use of different portions of the genetic material.
5)Apoptosis
 Cell does not divide or differentiate may undergo this
 A form of cell death that is normal part of development.
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