ENV 107L

Environmental Science Lab

Class 1

Department of Environmental Science and

Management
North South University (NSU)
Basic laboratory rules and safety
List of Do's and Don'ts in the Laboratory

• Students are not permitted to enter into the lab without the
permission of laboratory supervisor or course instructor.
• Personal belongings (i.e. Bags) should be placed in designated
areas.
• Considering the pandemic students are not allowed to enter the
laboratory without wearing a mask and students are
encouraged to wear head cover and hand gloves in the lab.
• Laboratory should be in clean condition after using it. No waste
is to be left in the sinks or desks. Waste (i.e. chemical, biological
or items such as broken glass, hand gloves) should be place in
the marked bins in the laboratory.
List of Do's and Don'ts in the Laboratory

• Defective equipment or broken glassware must be reported to the

tutor.
• Drinking and eating is not allowed inside the lab.
• Wash hands properly before leaving the laboratory.
• Report any kind of injuries to the instructor
 Average Calculation
• If there are six apples in a basket and their weights are 20 gm, 15
gm, 25 gm, 18 gm, 21 gm and 23gm: the average weight or
arithmetic mean is 20.33 gm.
Calculation:   gm =20.33 gm
 Percentage calculation

•  
 Graphing

• In each study there is one dependent variable and one or more

independent variables.
• The dependent variable is affected by what happens to the
independent variable.
• In a graph, the dependent variable is on the vertical (Y) axis and
the independent variable is on the horizontal axis (X).
Anatomy of a Line Graph
Anatomy of a Table
Experimental Section
For this lab exercise, students
will be given data about
marbles of different colour
during the class. The students
have to assume that the
marbles are given in a box and
the boxes will represent any
arbitrary part of the
environment, for example, a
random sample of water from
Hakaluki Haor, and the different
colored marbles will represent
different species of fish.
 Procedure:
1. Fill in Table 1.1 – and Table 1.2
2.Read over the questions and try to find out the answers for all the
questions for this lab.
3. Submit your report after you are done. You have 2 days to submit
your report in google classroom. You will work in a group of 2 people.
Ask your instructor for your group mate.
4. Type your report in word and then convert it to pdf format. Submit
your report in both word and PDF format.
 
Questions
Q1. Fill in data Table 1.1. In the missing information of the table. Give the table a title.

Q2. Fill in data Table 1.2. Give the table a title.

Q3. What percentage of the total number of marbles in YOUR (Suppose that you belong to any
of these four groups and mention which one) sample was each of the colours? Make a chart of
the percentages of each colour in your sample. Be sure to label your graph properly (including
label your data) in Microsoft Excel, and then copy it to Microsoft word.

Q4. Make a chart for the average of all the four groups showing the percentages of each colour
marble.

Q5. If there are 9536 marbles in the large container in the front of the room, a) how many
marbles would you predict be red based on your sample, and b) how many marbles would you
predict be red based on the average of four groups?

Q6. If your sample contained 1000 litres of water from Hakaluki Haor and the volume of
Hakaloki Haor is 125,000,000 cubic meters, and each of the marbles represents fish of that
colour, how many blue fishes would you expect to find in the whole Haor based on your
sampling? Be sure to include your calculations in your answer. 
Table 1.1
Group Total # of # of white # of purple # of blue # of red # of green # of
Number marbles marbles marbles marbles marbles marbles yellow
marbles

1 34 32 35 42 47 52

2 78 43 85 75 84 57

3 56 54 73 65 63 75

4 63 74 45 88 54 82

Average
 Table 1.2
Group Total % of % of % of % of blue % of red % of % of
Number marbles white purple marbles marbles green yellow
marbles marbles marbles marbles

Average
Sample solution for question 5
 
 Sample solution

• Sample solution for question 6:

We know that 1000L= 1m3
Total volume of Hakaluki Haor is 125,000,000 (m3) cubic meter [Given]
Suppose that your sample contains 89 blue fish
Then based on your sample the Hakaluki Haor will contain 89 X
125,000,000 =1.1125 X 1010 blue fishes
Thank you ☺