Sidang Internal Naskah Proposal

Sidqi Haiqal Irfansyah

NIM. 1913016032
Undergraduate thesis Supervisor : Dr. Angga Cipta Narsa, M.Si.,
Apt.
Undergraduate thesis Co. Supervisor : Nur Masyithah Z, M.
Farm., Apt.

Faculty of Pharmacy
Mulawarman University
2022
Undergraduate Thesis Title

Isolasi dan Karakterisasi Fraksi Etil

Asetat Dari Akar Bajakah Merah
(Uncaria nervosa Elmer)
Research Background
Indonesia has a variety of flora and fauna spread
troughout Indonesia. The plants found in Indonesia
are famous for their function as traditional
medicines. Bajakah (Uncaria nervosa) showed
cytotoxic, antiinflammation, antivirus, antibacterial,
antioxidant and immunostimulant activity (Maulina
et al, 2019)
Uncaria nervosa are plants with brownish-colored
stems that grow vines, and have large and strong
stem. Bajakah can usually grow more than 5 meters
and bajakah only grows in shady places with little
sun exposure (Lismana et all, 2022)

Bajakah is also known in Dayak language as roots,

and it is make bajakah not included in certain plant
species (Maulina et al, 2019)
Research Background

Plant classification

Kingdom : Plantae
Phylum : Tracheophyta
Class : Magnoliopsida
Order : Gentianales
Family : Rubiaceae
Genus : Uncaria
Species : Uncaria nervosa Elmer
Research Background
Isolation is a technique of separating compounds
contained in a natural material which are usually
secondary metabolites. Isolation consists of several stages
starting from the extraction process, fractionation, then
purification and identification.

Ethyl acetate is a solvent with semi-polar properties so

that it can attract polar and non-polar compounds, has
low toxicity, and is easily evaporated (Amelia and
Ngazizah, 2021). According to Harborne (1987) in Gazali
(2019) compound alkaloids, phenolics, and steroids can
be soluble in semi-polar solvents (ethyl acetate).
Therefore, a semi-polar solvent (ethyl acetate) is used in
order to attracts both polar and non-polar compounds
Formulation of the problem
1. How much is the yield of Uncaria nervosa ?

2. What secondary metabolites group are contained in ethyl acetate

fraction of Uncaria nervosa ?

3. What are the characteristics of the isolate compound of Uncaria

nervosa ?

4. What is the structure of the chemical compound from Uncaria

nervosa ?
Research purpose
General purpose of
research
Increase knowledge of natural
pharmaceuticals, and as reference
or comparison material for further
researchers and as information
about Uncaria nervosa from ethyl
acetate fraction
Special purpose of
research
 Knowing the yield of Uncaria
nervosa
 Determine the secondary
metabolites group contained in
the ethyl acetate fraction from
Uncaria nervosa
 Knowing the characteristics of
isolate compounds of Uncaria
nervosa
 Knowing the structure of the
isolated chemical compounds
contained in Uncaria nervosa
 Research methods
Sample of Fresh sample
• Calculate Identification
yield Uncaria of Uncaria
plant
• Phytochem nervosa nervosa
ical
screening
• Powder
• Liquid-
Separation by ed
liquid
• VLC
fractionatio various Simplic
chromatographic
Extract Simplicia ia
n
Fraction
separation techniques • Simplic
• CC ia
Fraction extracte
separation d using
• Eluent Charaterization macerat
Chemical
determinati Isolate by various ion
compound method
on compound spectroscopic
methods structure
• Purificatio
Research methods (Sample
Preparation)
Sample of
Uncaria Collected Wet Sorting
nervosa

Uncaria
Chopped nervosa x Weighed
gram

Uncaria
Dry sorting nervosa Y
gram
Research methods (Sample
extraction)
Powdered Simplicia, Simplicia Partitioned
Sample of
extracted using maceration Concentrated using
Uncaria
method and Concentrated using extract water:n-
nervosa hexane
rotary evaporator

Water
Take water phase,
Concentrated Ethyl acetate phase, phase
then partitioned
ethyl acetate concentrated using rotary and n-
using water:ethyl
phase evaporator hexane
acetate
phase

Separation and purification by Characterization

Pure by spectroscopy Charaterize
various chromatographic methods
Isolate (UV-Vis, IR, d isolate
and recrystalization NMR, and MS)
Research methods (Phytochemical
Screening)

Appropriate
Put into the
Extract reagent is
reagent tube
added

Secondary
Watch the color
metabolites
change
group
 Data Collection Technique
Phytochemical Screening Research Design Best eluent determination research
Table design table
No Test Methods Reagent Test Test Result based on No. Eluent Eluent TLC Description
. Result literature comparison Result
1 Flavonoid Mg powder  
and 1        
Concentrated
HCl 2        
2 Alkaloid Dragendorff,   3        
Mayer, and
Wagner 4    
   
3 Saponin Aquades  

4 Phenolic FeCl­3  
The expected results of the research are the yield of red
bajakah root extract (Uncaria nervosa Elmer), the secondary
metabolite group found in red bajakah root (Uncaria
nervosa Elmer), isolates from secondary metabolites and
characterization of isolate compounds from red bajakah root
(Uncaria nervosa Elmer). ).

Expected Results
1.Thank You