Biofilm in Vegetables cleaning

and washing Jacuzzi

Biofilm Detection & Remove Trial

Barcelona - July 2015

Ariel Mlynski-Zohar Dalia -Israel

Target and reasons for the participation
In the convention
 Zohar Dalia- 7 Business Unit
Production capacity of 80,000 tons
Annual sales 100 million dollars
300 employees Among our customers: Unilever, Henkel, RB, BASF, SANO, Coca Cola, Nestle…

Institutional Agro Food & Beverage Water Care

Health Care Automotive Industry

 Biofilm in Vegetables cleaning
and washing Jacuzzi

The problem 1.Lack of clear and “easy" cleaning and sanitizing

procedures to avoid microbiological contamination.
2. Lack of knowledge about BF

3. Tough listeria m. regulation (negative/25 gr sample)

The symptom Shortening shelf life and moulds spots/leaves

fermentation .
The result: sudden microbiological peaks including pathogens lysteria M
 Biofilm in Vegetables cleaning
and washing Jacuzzi

The consequences: Silent or public recall

Ministry of Health involvement

Public recall=150000 euros

 Cleaning Trial-Jacuzzi Vegetables

Target: Remove Biofilm with TENSIO CIP and BIO CIP by circulation
pipes after finding positive Biofilm with BIOFINDER

Cleaning protocol
Normal cleaning was conducted with our regular products. The cleaning product was
circulated 15 min more to try to remove the Biofilm.

The Jacuzzi was filled with water 50-53 C. Approval from the Jacuzzi's supplier was
got before to allow use of high temp (pumps sensitivity).

TENSIO CIP and BIO CIP were added manually 0.25% and 0.05% Respectively
 Biofilm in Jacuzzi-Sensitive points

Inside parts

Welded non
dismountable
pipes

screws
External Internal
screws pipes
 Biofilm in Jacuzzi-Sensitive points

Dead end
Low circulation areas
 Preparation and Cleaning Protocol

4. Circulation for 35 min. End temp 45 C.

5. During circulation were added to the Jacuzzi, external instruments

that were “BIOFINDER POSSITIVE” but could not be cleaned by
normal procedure
6. Rinsing with potable water.

7. Sanitizing with 150 PPM Chlorine by circulation for 10 min

8. Results
Results – Outside parts
External screw

BF POSSITIVE

After cleaning
Results – External parts

BF POSSITIVE
After cleaning
 Results – Inside parts

After cleaning
After cleaning

BF POSSITIVE

BF POSSITIVE
 Results – End Pipes
After cleaning

BF POSSITIVE

Note: the end-pipe was lightly opened to allow circulation out

 Results – Crates

After cleaning

BF POSSITIVE

Note: crates were cleaned 3 times in a crate washer before

detecting with BIOFINDER
 Results -Conclusions

Shelf life of product increase from 9 to 12 days

1. Biofilm was removed from Jacuzzi sensitive-positive-parts including
external ones by the TENSIO CIP and BIO CIP.

2. There is need to supply HOT water in a easy way to allow regular

(weekly BF removing). An equipment investment will be needed.

3. Each equipment / factory will need to create monitoring program

to validate BF frequency removing
 Conclusions

4. Factories (food technologist) still do not understand the differences

between “regular dirtiness” and BF presence!!!!!

5. QAM ( mainly food technologist) tend to blame our chemicals

without understanding BF can not be removed by normal
chemicals !!!!!

?Questions