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Enzyme Class2
Enzyme Class2
What is an enzyme?
globular protein
which functions as
a biological
Active
catalyst, speeding
site up reaction rate by
lowering activation
energy without
being affected by
the reaction it
catalyse
Enzymes are protein in nature (?)
Globular protein.
Ribozymes are RNA molecule with
enzymatic activity.
Catalytic behaviour of any enzyme
depends upon its primary, secondary,
tertiary or quaternary structure.
Enzymes of digestive tract and those
found in blood are present in inactive form
called zymogen or proezymes.
Active site
Enzymes are composed of
long chains of amino acids that
have folded into a very specific
three-dimensional shape which
contains an active site.
e.g. O2 + H2O
Progress of Reaction
Nomenclature / enzyme classification
IUBMB has recommended system of
nomenclature for enzymes & according to them
each enzyme is assigned with two names:
-
Factors affecting reaction velocity
Temperature
Hydrogen ion concentration (pH)
Substrate concentration
Enzyme concentration
Products of the reaction
Presence of activator/inhibitor
Allosteric effects
Time
Effect of Temperature
Reaction
Velocity
(v0)
Temperature(oC)
Effect of pH
Trypsin
Pepsin
Reaction
Velocity
(v0)
q r
pH
Rate of the reaction or velocity is directly
propostional to the Enzyme Concentration
when sufficient substrate is present.
Reaction
Velocity
(v0)
1 Km 1 1
v Vmax [ S ] Vmax
Enzyme Inhibiton
Any substance that can diminish the velocity
of an enzyme catalyzed
These include drugs, antibiotics, poisons,
and anti-metabolites.
Useful in understanding the sequence of
enzyme catalyzed reactions, metabolic
regulation, studying the mechanism of cell
toxicity produced by toxicants.
Forms the basis of drug designing.
Types of Enzyme Inhibiton
Reversible inhibitors
Irreversible inhibitors
Reversible inhibitors can be classified
into :
Competitive
Non-competitive
Un-competitive
Competitive Inhibition
Non-Competitive Inhibition
Un-competitive Inhibiton
ES Complex
Enzyme
+ Inhibitor
ESI complex
Enzyme Inhibition (Plots)
I I I