ORGAN CULTURE

DWI RETNO ARYATI, SP

Dr Ir DINY DINARTI, MSi
Basic Methods of In vitro Popagation for

1. Enhanced axillary shoot

proliferation (shoot Pre-existing
culture; meristem culture) meristem
2. Node culture
Node culture: a simplified form of shoot
culture, is another method for
production from preexisting meristem.
 Factors affecting tip culture :
1. Culture media
2. Plant Growth Regulator
3. Plants
 Type of plant
 Plant’s age
4. Growth environment
 Light
 Temperature
Culture Medium
 Most of horticulture plants using MS
media, while woody plants using WPM
(Woody Plant Medium).
 The choose of medium on tip culture need
to focus on plant’s need
 young or old plant
 herbacious or woody plant
Plant Growth Regulator
 The type and concentration of PGR determine
of the stage and type of growth from plant
 Mostly used PGR on tip culture is Auxin and
Sitokinin
 For Auxin : NAA and IBA
 Sitokinin : BAP, 2ip and Kinetin
 To induced and propagated the shoot :
Sitokinin >>>> Auxin
Plants
 Type of plant, herbacious or woody needs
different treatment
 potato’s tip : MS medium
 orange’s shoot : WPM

 Plant’s age determine the PGR

 Plant’s condition; vigorous, health affecting
the tip meristem successfull
 Environment
 Potatoes needs cold temperature (18), while
sweet potatoes needs more warm
temperature (24)
 Light for plants less needed for
organogenesis and embryogenesis. Light
needed for morfogenesis
 2 Embryo Culture
 Embryo culture has been used to
help the germination.
 Sometime used to callus initiation
Embryo Stage :
Embryogenesis
Jathropa curcas
Somatic Embryogenesis of Coffea
Embryo culture needed for embryo with
problems, such as :
1. Long time of dormancy
2. Hibridization interspesific embryo which
incompatible with its endosperm
3. Embryo without endosperm (Orchid)
4. Embryo with damaged endosperm
(kopyor)
Post zygotic incompatible problems that
overcome with embryo rescue, known as
EMBRYO RESCUE

• Raghavan (1966) divided the embryo stage

on 2 phase :
1. Heterotrophic phase
Globular - early heart stage
1. Autotrophic phase
End of heart stage