Green synthesis of Silver nanoparticles from

Allium Cepa extract for cancer cell treatment

Silver nanoparticles
Malik Abdul salam SP16-BPH-015
Characterization
UV Spectroscopy

Nanotechnology
Green Synthesis
Green Synthesis

Allium Cepa
Preparation
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Formation

Supervisor : Dr. Muhammad Hammad Aziz

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of NPs

Co. Supervisor : Dr. Mukhtar Ahmad

X-ray Diffraction
UV Spectroscopy
Characterization
Formation
Preparation
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Green Synthesis
of NPs
Green Synthesis
Silver nanoparticles
Allium Cepa
Nanotechnology
Reference
Conclusion
Contents

Green synthesis
Nanotechnology

Characterization
Experiment work
Green synthesis Ag NPs

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 Reference
Conclusion
X-ray Diffraction
UV Spectroscopy
Characterization
Formation
Preparation
Advantages
Green Synthesis
of NPs
Green Synthesis
Silver nanoparticles
Allium Cepa
scale.
Definition:
Nanotechnology

metric system equal to one

billionth of a meter (10-9m)
manipulating matter on nano

Nano is a unit of length in the

Nanotechnology is the study of

Nanotechnology
 Allium Cepa

Allium cepa most widely cultivated and its bulb is used

as both food and medicine.Allium cepa have various

Silver nanoparticles
biological properties like antibiotic, antidiabetic,
Characterization
X-ray Diffraction

Green Synthesis

Green Synthesis

Nanotechnology
UV Spectroscopy

antioxidant , antiatherogenic and anticancer.

Allium Cepa
Preparation
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Formation
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Reference

of NPs
 Silver nanoparticles

AgNPs play a significant role in the field of diagnostic

medicine antimicrobial synthesis and therapeutics.

Silver nanoparticles
Ag+ contributes to the antimicrobial ability of AgNPs
Characterization
X-ray Diffraction
UV Spectroscopy

Green Synthesis

Green Synthesis

Nanotechnology
under various hypothesis including, breakdown,

Allium Cepa
Preparation
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Formation
Conclusion

permeabilization of cell membrane, and interaction with

of NPs proteins and DNA.
 Green Synthesis

Green synthesis is the utilization of a set of

principles that’s reduces or eliminates the use of

Silver nanoparticles
hazardous substances in the design and
X-ray Diffraction

Characterization

Nanotechnology
Green Synthesis
UV Spectroscopy

Green Synthesis
manufacture of chemical products

Allium Cepa
Preparation
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Conclusion

Formation
Reference

of NPs
 Green Synthesis of NPs

•Green synthesis of nanoparticles by plants and extract are

the green chemistry approach that interconnects

Silver nanoparticles
nanotechnology and plant biotechnology
Characterization
X-ray Diffraction

Nanotechnology
UV Spectroscopy

Green Synthesis
Green Synthesis
•Plant extract are used for the metal ions bio-reduction to

Allium Cepa
Preparation
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Formation

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form nanoparticles

of NPs
 Advantages of Green synthesis

•Environment friendly
•Easily scaled up for large synthesis of nanoparticles

Silver nanoparticles
•No need of high temperature pressure , energy and toxic
Characterization
X-ray Diffraction
UV Spectroscopy

Green Synthesis
Green Synthesis

Nanotechnology
chemicals

Allium Cepa
Preparation

Advantages
Formation

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•More advantages over use of micro-organisms by less

of NPs
elaborate process of maintaining cultures
•Reduce cost of micro-organism isolation and their culture
media
 Preparation of extract

•Washed the onion 5 times from distilled water

•Cut the onion in to small pieces

Silver nanoparticles
•Take 20ml distilled water and 1 gram onion
X-ray Diffraction

Characterization
UV Spectroscopy

Green Synthesis

Nanotechnology
Green Synthesis
•Boil the onion in distilled water for 60min

Allium Cepa
Advantages
Preparation
Formation

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•Cool down the solution and filter

of NPs
•Take the silver nitrate o.98g and 10ml distilled water
and stirrer for 45 min
•Add silver nitrate solution and onion solution with each
other and continuously stirrer for 3 hours
•Heat the mixture in oven at 180 degree for 3 hours
 Flow Chart
Prepared
sample for
further use
Silver Distilled Onion
nitrate Water Extract

Silver nanoparticles
Grinding by
Characterization

Mortar &

Green Synthesis
Green Synthesis

Nanotechnology
Allium Cepa
pestle

Preparation
Flow Chart

Advantages

Contents
of NPs
Silver nitrate 0.98g in Add extract drop
10ml distilled water wise in silver nitrate
solution Calcination
at 180 ⸰C

Heat in
Mixture oven
stirrer for 3hr
Filter the
continuously
mixture with
at room
filter paper
temperature
 Characterization

Silver nanoparticles
X-ray Diffraction
UV Spectroscopy

characterization

Green Synthesis
Green Synthesis

Nanotechnology
Allium Cepa
Preparation
Conclusion

Advantages
Formation
UV spectroscopy : Electromagnetic radiation

Contents
of NPs
 with wavelength from 100 nm to 400 nm shorter than that
of visible light but longer than X-ray

XRD :  is a non-destructive  x-ray method used to analyze

the structure and size of crystalline materials
 X-ray Diffraction
Ultra-violet spectroscopy

www.sciencedirect.com/science/article/pii/S0141813018346907

UV Spectroscopy
Characterization
Formation
Preparation
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Green Synthesis
of NPs
Green Synthesis
Silver nanoparticles
Allium Cepa
Nanotechnology
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 X-ray diffraction

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5822509/

X-ray Diffraction
UV Spectroscopy
Characterization
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Preparation
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Green Synthesis
of NPs
Green Synthesis
Silver nanoparticles
Allium Cepa
Nanotechnology
Contents
 X-ray diffraction

act_and_their_characterization
ynthesis_of_silver_nanoparticles_using_Ocimum_leaf_e
ps://www.researchgate.net/publication/259004979_Gree
FTIR
X-ray Diffraction
UV Spectroscopy
Characterization
Formation
Preparation
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Green Synthesis
of NPs
Green Synthesis
Silver nanoparticles
Allium Cepa
Nanotechnology
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Conclusions:

Silver nanoparticles
Characterization
X-ray Diffraction
UV Spectroscopy
In this work we have reported a simple eco-friendly

Green Synthesis
Green Synthesis

Nanotechnology
preparation of silver nanoparticles by using Allium Cepa as

Allium Cepa
Preparation
Advantages
Conclusion

Formation

Contents
a reducing and stabilizing agent

of NPs
In XRD analysis average crystallite size of the sample was
obtained 28nm. These above results showed that as
prepared AgNPs particles were in the nano-range.
Reference
• Prasad R. Synthesis of silver nanoparticles in
photosynthetic plants. J Nanopart 2014. 2014:8.
• Ge L, Li Q, Wang M, Ouyang J, Li X, Xing MM, et al.
Nanosilver particles in medical applications: Synthesis,

Silver nanoparticles
performance, and toxicity. Int J Nanomedicine. 2014;9:2399–

Characterization
407

X-ray Diffraction
UV Spectroscopy

Green Synthesis
Green Synthesis

Nanotechnology
• Kalidindi N, Nandeep R, Swetha S, Kalidindi B. Antifungal

Allium Cepa
Preparation
Advantages
Conclusion

Formation
Reference

Contents
and antioxidant activities of organic and aqueous extracts

of NPs
of Annona squamosa linn
•. Ge L, Li Q, Wang M, Ouyang J, Li X, Xing MM, et al.
Nanosilver particles in medical applications: Synthesis,
performance, and toxicity. Int J Nanomedicine.
• Calderone RA, Fonzi WA. Virulence factors of Candida
albicans. Trends Microbiol. 2001;9:327–35
•A. R. Allafchian , S. Z. Mirahmadi-Zare , S. A. H. Jalali , S. S.
Hashemi and M. R. Vahabi , J. Nanostruct. Chem., 2016