• Gene can be synthesized in laboratory from

messenger RNA by using.

• Restriction enzymes
• cDNA
• vector
• reverse transcriptase
• When two different pieces of DNA are joined
together, the result is which one of the
following.
• Complementary DNA
• Redundant DNA
• Cloned DNA
• Chimaeric DNA
• Cystic fibrosis affects which one of the
following cells of the body.
• Epithelial cells
• endothelial cells
• Plasma cells
• blood cells
• In cystic fibrosis, liposomes-microscopic
vesicles are used which are coated with:
• A) Healthy Gene
• B) Protein
• C) Chromosome
• D) Carbohydrate
• Bacterial cells take up recombinant plasmids
when they are treated with:
• A) CaCl2
• B) KCl
• C) NaCl
• D) NaOH
• Which one of the following is made up of
radioactively labelled nucleotides?
• A) Phage DNA
• B) Recombinant DNA
• C)Primer
• D) Gene Probe
• Temperature insensitive (thermo stable)
enzyme used in PCR is.
•  DNA polymerase 1
• DNA polymerase 111
• DNA ligase
• taq polymerase
• Antigen to treat non-Hodgkin’s lymphoma are
produced by.
• Wheat plant
• rice plant
• tobacco plant
• corn plant
• Liposomes are used in gene therapy against.
• Hypercholesterolemia
• cystic fibrosis
• severe combined immune deficiency syndrome
• Cancer
• Genetically engineered cells are introduced into
bone marrow cells in the treatment of.
• Hypercholesterolemia
• cystic fibrosis
• severe combined immune deficiency syndrome
• coronary artery angioplasty
• In DNA finger printing process the use of
………………. Produces distinctive pattern on
autoradiography or X-ray film.
• Restriction enzymes
• Primeres
• Probes for genetic markers
• Electrophoresis
• The agent which separates the two strands of
DNA in PCR is.
• DNA ligase
• primer
• helicase
• heat
• Restriction endonucleases cleave the
__________ of duplex DNA.
• A) Nitrogenous base
• B) Phosphodiester bond
• C) Base sugar
• D) Hydrogen bond
• The enzyme which is responsible for the
formation of bond between two double
stranded DNA fragments is:
• A) Endonuclease
• B) Ligase
• C) Urease
• D) Helicase
• DNA fragments of different lengths can be
separated by a process of
• Autoradiography
• Gel electrophoresis
• Northen blotting
• RFLPs
• All of the following consist of DNA except?
• Primer
• Probe
• cDNA
• Gene of interest