GURU NANAK INSTITUTE OF PHARMACEUTICAL SCIENCE AND

TECHNOLOGY (An Autonomous Institute)

TOPIC : STREAK AND POUR PLATE METHOD

NAME OF THE STUDENT: SOUVIK CHATTERJEE PAPER NAME:PHARMACEUTICAL MICROBIOLOGY

PNR NUMBER: 186012211010 PAPER CODE: R21_PT319

ACADEMIC SESSION: 2022-2023

Affiliated to Maulana Abul Kalam Azad University of Technology

 INTRODUCTION

Mixed culture media: A culture media, which contain more than one kind of
microorganism is called a mixed culture media.

A culture that contain only are Kind of microoganism

Pure culture media:
is called pure culture media.

Isolation of pure culture:

The isolation of this only one kind of microorganism from a mixture culture media is called Isolation
of pure culture.

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 Streak-plate method

Pour-plate method
method of pure
culture Serial dilution method

Spread plate method

Streak-plate method Pour-plate method

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Streak-plate method Streaking is the process of spreading the microbial culture with an
inoculating needle on the surface of the media.

 Examine the streak plates prepared and locate a number of well-isolated

Procedure colonies.
 Now you must transfer a portion of each colony to a separate agar slant.
 To “pick” a colony you will be using an inoculation loop.
 Sterilize the loop in the burner flame, let cool 3-5 seconds then touch the
end of the loop to the isolated colony, picking up the microorganisms from
the colony.
 Now recover the streak plate and pick up one NA plate.
 You will now be holding the inoculating loop in your right hand and the
fresh NA plate in your left hand.
 Remove the lid from the plate, place the inoculating loop at one edge of
the plate and with a sweeping strock, and inoculate the agar using the
same tri-streak method as used for the initial isolation.
 Replace the lid.
 Flame the loop and proceed to inoculate another plate from different
colonies. Try to use colonies that are visibly different in morphology.
 Incubate the plates in 37° c incubator.
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 The pour plate method is a microbiological laboratory technique for
isolating and counting the viable microorganisms present in a liquid
Pour-plate method: sample, which is added along with or before molten agar medium
prior to its solidification.

Procedure • Take the bacterial culture and

liquid agar medium.
• Mixed together
• After mixing the medium
containing the culture Poured
into sterilized Petri dishes.
• Then allowed solidifying
• Then incubated
• After incubation, the colonies
appear on the surface.

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 REFERENCE

• Hugo and Russell's Pharmaceutical Microbiology, 8th Edition,Stephen P. Denyer (Editor), Norman A.
Hodges (Editor), Sean P. Gorman (Editor), Brendan F. Gilmore (Editor) ISBN: 978-1-444-33063-2
August 2011 Wiley-Blackwell 524 Pages.
• Pharmaceutical Microbiology Ist Edition Essentials for Quality Assurance and Quality Control Author:
Tim Sandle
• Prescott's Microbiology 10th EditionBy Joanne Willey and Linda Sherwood and Christopher J.
Woolverton .ISBN10: 1259281590 ISBN13: 9781259281594 Copyright: 2017

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