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Powerpoint To Accompany Concepts in Biology: Twelfth Edition Enger - Ross - Bailey
Powerpoint To Accompany Concepts in Biology: Twelfth Edition Enger - Ross - Bailey
Powerpoint To Accompany Concepts in Biology: Twelfth Edition Enger - Ross - Bailey
CONCEPTS IN BIOLOGY
TWELFTH EDITION
CHAPTER 8
Copyright © The McGraw-Hill Companies, Inc. Permission required for reproduction or display.
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8.1 DNA and the importance of
proteins
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8.2 DNA structure and function
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DNA structure
● DNA is a nucleic acid.
● Nucleic acids
– Large polymers made of
nucleotides
● A sugar molecule
– Deoxyribose for
DNA
– Ribose for RNA
● A phosphate group
● A nitrogenous base
– Adenine
– Guanine
– Cytosine
– Thymine
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DNA structure
5
DNA structure
● DNA is double
stranded.
– Held together by
hydrogen bonds
between the
bases
– A-T, G-C
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Base pairing aids DNA replication
● DNA replication
– is the process by which DNA is copied.
● This is done before cell division.
● Provides the daughter cells with a copy
of the genetic information
– relies on the base-pairing rules and many
enzymes.
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Base pairing aids DNA replication
– Is accomplished by DNA polymerase and other enzymes
1. Helicases (as enzymes) binds to DNA and forms a replication
bubble. And, helicases separate the two strands. (Fig. 8.3a &
8.3b)
2. DNA polymerase builds new DNA strands that will pair with
each old DNA strand. (Fig. 8.3c & 8.3d)
– Where there is an A on the old strand, polymerase will
add a T to the new strand.
3. In eukaryotic cells, replication process starts at the same time
in several places along the DNA molecule. As the points of
DNA replication meet each other, they combine and a new
strand of DNA is formed (Fig. 8.3 e)
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Repairing genetic information
● If a mistake is made
when building the new
strand
– The old strand still
has the correct
information.
– This information can
be used to correct
the new strand. (Fig.
8.4)
Fig.
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8.4
The DNA code
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HOW SCIENCE WORKS 8.1 (p.
148)
8.3 RNA structure and function
● RNA vs DNA
– RNA has ribose sugar
(DNA has deoxyribose).
– RNA contains the bases
● Adenine
● Guanine
● Cytosine
● Uracil (DNA has
thymine)
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DNA vs RNA
– RNA’s, like DNA’s, base sequence carries
information.
– RNA is made in the nucleus and transported to
the cytoplasm (DNA stays in the nucleus).
– The protein coding information in RNA
● Comes from DNA
– Like DNA replication, RNA synthesis follows the
base-pairing rules (A-U; G-C).
– RNA is single-stranded.
– Three types of RNA participate in protein
synthesis
● mRNA (nessenger)
● tRNA (transfer)
● rRNA (ribosomal)
8.4 Protein synthesis
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Transcription
● During transcription
– DNA is used as a template to make RNA.
– Accomplished by RNA polymerase
● Follows the base-pairing rules
● The process of transcription
– Begins in the nucleus
– RNA polymerase separates the two strands of
DNA.
– Only one of the two strands will be used to create
the RNA.
● The coding strand
● The other DNA strand is called the non-coding strand.
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Transcription of an RNA molecule
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The process of transcription
– Only a segment of the DNA strand will be used to create
each RNA.
● These segments are called genes.
● Each gene starts with a promoter.
– The RNA polymerase binds to the promoter to start
building an RNA strand.
● Each gene ends with a terminator sequence.
– The RNA polymerase will stop transcribing at the
terminator sequence.
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Translation
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The genetic code
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Translation
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Translation initiation
● Translation begins when
– The small ribosomal subunit binds to the
beginning of the mRNA.
– At this point, a tRNA brings the first amino acid.
● The anticodon in the tRNA matches with a
codon on the mRNA.
● Each tRNA carries a specific amino acid based
on its anticodon.
– The first tRNA binds to the start codon, AUG.
● This tRNA carries a methionine.
– Then the large ribosomal subunit joins the
complex.
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Initiation
Translation elongation
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Elongation
Translation termination
● Elongation continues until the ribosome
encounters a stop codon.
– UAA, UAG, UGA are stop codons.
● A release factor binds to the stop codon.
– This causes the ribosome to release the
polypeptide.
– The ribosomal subunits separate and release the
mRNA.
– The mRNA can be translated again by another
ribosome.
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Termination
Figure 8.10a
Summary of protein synthesis
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The genetic code is nearly universal
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Eukaryotic genome packaging
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OUTLOOKS
8.2
Telomeres
Control of protein quantity
– By increasing or decreasing the rate of
transcription of the gene by enhancer and silencer
regions on the DNA.
● Activation of enhancer regions increases transcription.
● Activation of silencer regions decreases transcription.
– Through the binding of transcription factors,
● these proteins bind to the promoter and facilitates RNA
polymerase binding and transcription.
– By limiting the amount of time the mRNA exists in
the cytoplasm,
● some mRNA molecules are more stable and will exist
longer in the cytoplasm, yielding more protein.
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Scientific American (Chinese)
No. 76, June, p. 56-69, 2008
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Transcription factors
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Control of protein quality
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Transcription of mRNA in eukaryotic
cells
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Alternative splicing
● Different
combinations of
exons from a
single gene can be
joined to build a
number of different
mRNAs for a
number of different
proteins.
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8.6 Mutations and protein synthesis
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Point mutations
● A change in a single nucleotide of the DNA sequence
● A silent mutation does not cause a change in the
amino acid sequence. (see Fig. 8.16)
– UUU to UUC; both code for Phe
● A nonsense mutation changes a codon to a stop
codon.
– This causes the ribosome to stop translation prematurely.
– CAA (Gln) to UAA
● A missense mutation causes a change in the type of
amino acid added to a polypeptide.
– This may change the way in which a protein functions.
– UUU to GUU; Phe to Val change
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Point mutations
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Sickle cell anemia
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Insertions and Deletions
● An insertion mutation occurs when one or
more nucleotides is added to the normal DNA
sequence.
● A deletion mutation occurs when one or more
nucleotides is removed from the normal DNA
sequence.
● Insertions and deletions cause a frameshift.
– Ribosomes will read the wrong set of three
nucleotides.
– Changes the amino acid sequence dramatically
– Changes the function of the protein dramatically
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Frameshift
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● Insertions cause a frameshift.
– Some viruses, such as HIV, can insert
their genetic code into the DNA of their
host organism