GLV’s Quality Aspects and Development in

Analysis
 OVERLOOK
 Fresh Green Leafy Vegetables naturally deteriorate after harvest, and its rate of
deterioration is affected by a variety of factors, including respiration, ethylene
production and action, chemical changes, water loss, physiological disorders, and
microbial breakdown

 Produce that is subjected to non-optimum storage temperature and humidity,

mechanical damage demonstrates increased rate of deterioration.

 GLV are rich in micronutrients like folic acid and carotenoids and also has high
antioxidant capacity.
 QUALITY CHARACTERISTICS OF GLV
 Water Loss
• We can find out the Water Loss of the respective GLV by keeping it for some duration of days.
• Mark the GLV’s weight on day 0 as (W0) of each experiment and mark it (WA) on each
analysis day.
• The water loss of each day can be calculated using the following formula:
• Water Loss (WL %) = (1 – W0/WA) · 100.

 LEAF TENDERNESS
• The tenderness of the spinach leaves can be measured using a texture analyzer TA-58, TA.XT.
plus (Texture Technologies equipped with a 5-blade TA-91 Kramer Shear Cell.
• The return distance of the probe would be set at 35 mm, the test speed at 1.7 mm/s, and the
return speed can be at 10 mm/s.
• For each measurement, the midribs of 10 leaves per replication have to be removed and the
leaves should be stacked and weighed.
• The maximum force required to cut through the GLV’s stack is measured similar
• Leaf tenderness is calculated as the maximum force in Newtons per gram (N/g).
 Chlorophyll Content
• https://www.youtube.com/watch?v=O3l85nnwgPg
• Some results of researches have revealed that the SPAD values differ from photometric
measurements of solvent-extracted chlorophyll by just ~6% on average.
 Electrolyte leakage
• The electrolyte leakage of GLV is measured by placing the leaf in 20 mL of deionized
water in a 50-mL tube and the electrolyte leakage was measured immediately (this value is
denoted as EC0) using a conductivity meter (OHAUS Corporation, Parsippany, NJ).
 Nutritional Quality
• The nutritional quality of the GLV can be evaluated by measuring total phenolic content,
antioxidant capacity, and vitamin C content.
 Leaf surface color
• The color of GLV’s leaves can be measured using an A5 Chroma-Meter Minolta CR-400.
• The color measurements were taken on the upper part of the leaf at two opposite points to
the central leaf axis.
• Color results are expressed in the CIE L*a*b* color space, where L* is lightness and h is
hue angle, calculated as tan–1 b*/a*
 DISCUSSION

 To find out unconventional ways in which we can analyse the Nutritional

Qualities.
 To find the relation between the nutritional values with the Quality
Parameters
 Feasibility of instruments noted in the PPT.
 TO BE UNDERSTOOD
 AIM-Use of a SPAD-502 meter to measure leaf chlorophyll concentration in
Arabidopsis thaliana
 The SPAD-502 meter is a hand-held device that is widely used for the rapid, accurate
and non-destructive measurement of leaf chlorophyll concentrations. It has been
employed extensively in both research and agricultural applications, with a range of
different plant species. However, its utility has not been fully exploited in relation to
the most intensively studied model organism for plant science research, Arabidopsis
thaliana. Measurements with the SPAD-502 meter produce relative SPAD meter
values that are proportional to the amount of chlorophyll present in the leaf. In order
to convert these values into absolute units of chlorophyll concentration, calibration
curves must be derived and utilized. Here, we present calibration equations for
Arabidopsis that can be used to convert SPAD values into total chlorophyll per unit
leaf area (nmol/cm(2); R(2) = 0.9960) or per unit fresh weight of leaf tissue
(nmol/mg; R(2) = 0.9809). These relationships were derived using a series of
Arabidopsis chloroplast biogenesis mutants that exhibit chlorophyll deficiencies of
varying severity, and were verified by the subsequent analysis of senescent or light-
stressed leaves. Our results revealed that the converted SPAD values differ from
photometric measurements of solvent-extracted chlorophyll by just ~6% on average.