RT-PCR in the treatment of COVID-19

Author’s Name : Kunal Tripathi *, Raj Kumari & Meenakshi Sharma

ITS College of Pharmacy, it’s The Education Group, Ghaziabad, Uttar Pradesh.
Email : tripathikunal27@gmail.com

Poster No.: IC/SDPC/23/99 RESULT &DISCUSSION

ABSTRACT RT-PCR do not involve AI directly but as can be seen in the methodology
 Reverse transcription polymerase chain reaction (RT-PCR) is a laboratory have auto/self temperature regulation as an important functioning the
technique combining reverse transcription of RNA into DNA and diagnosis of COVID-19.But , this is not still giving 100% accurate testing
amplification of specific DNA targets using polymerase chain reaction reports so Chest-CT may be referred as it is an complete AI based diagnosis
(PCR).It is primarily used to measure the amount of a specific RNA. This is which can further be taken to qure.ai which is completely AI based &
achieved by monitoring the amplification reaction using fluorescence, a doesn’t require human efforts.
technique called real-time PCR or quantitative PCR (qPCR). Combined RT- On moving further to the treatment of COVID-19 humanoids can help in a
PCR and qPCR are routinely used for analysis of gene expression and variety of field such as hospitals for treatment & manufacturing of drugs.
quantification of viral RNA in research and clinical settings. RT-PCR The
close association between RT-PCR and qPCR has led to metonymic use of
the term qPCR to mean RT-PCR. Such use may be confusing, as RT-PCR can
be used without qPCR, for example to enable molecular cloning,
sequencing or simple detection of RNA. Conversely, qPCR may be used
without RT-PCR, for example to quantify the copy number of a specific
piece of DNA.

INTRODUCTION
Coronavirus
  disease (COVID-19) is an infectious disease caused by the SARS-
CoV-2 virus.

Most people infected with the virus will experience mild to moderate
respiratory illness and recover without requiring special treatment.
However, some will become seriously ill and require medical attention.
Older people and those with underlying medical conditions like
cardiovascular disease, diabetes, chronic respiratory disease, or cancer are
more likely to develop serious illness. Anyone can get sick with COVID-19
and become seriously ill or die at any age.
The virus can spread from an infected person’s mouth or nose in small liquid
particles when they cough, sneeze, speak, sing or breathe. These particles
range from larger respiratory droplets to smaller aerosols. It is important to
practice respiratory etiquette, for example by coughing into a flexed elbow,
and to stay home and self-isolate until you recover if you feel unwell. CONCLUSION
1)The conclusion is that AI can be a lot beneficial in the field of
diagnosis & treatment of diseases in human beings.
Methodology 2)Because diseases such as COVID-19 can be treated diagnosed easily
The sample is processed with a variety of chemical solutions that
& thus can increase the chances of life expectancy because “EARLY
extract just the RNA contained in the sample while removing other DETECTION IS BETTER TREATMENT ”.
components like proteins and lipids. 3)In treatment of diseases such as COVID-19 AI functioned
The RT-PCR apparatus is subsequently filled with the mixture. humanoids/robo’s can be a lot beneficial in taking care of the
The device alternately heats and cools the liquid to start precise patients. As they will decrease the chances of disease widespread
chemical processes that duplicate the targeted viral DNA segments in among humans as they won’t act like a carrier & can also be
fresh, identical copies. sterilized.
To keep duplicating the targeted viral DNA regions, the cycle is 4)AI is currently not given such status in the Indian traditional society
repeatedly repeated. The previous number is doubled with each but it would be better if we accept the future but on our condition.
cycle: two copies become four, four copies become eight, and so on.
By the time a typical real-time RT-PCR setup has completed 35 cycles,
REFERENCES
each strand of the virus present in the sample has produced about 35 • Ai Tang Xiao, Yi Xin Tong, and Sheng Zhang. Dynamic Profile of RTPCR
billion additional copies of the viral DNA segments. finding from COVID 19 patients in Wuhan, China : A descriptive study.
• Marco Ciotti, Massimo Ciccozzi, Alessandro Terrinoni, Wen-Can Jiang,
*RT-PCR may sometime not give 100% accurate result ,so at the time Cheng-Bin Wang and Sergio Bernardini , research on the COVID 19
Chest CT Scan may be preferred for accurate results. pendemic.
• www.who.int

Presented at the “International Conference (Hybrid Mode) on ­the "Artificial Intelligence in Business, Management and Pharmaceutical
Technology'” held at Sunder Deep Group of Institutions, Dasna, Ghaziabad on 18 th-19th February 2023.