Professional Documents
Culture Documents
Chapter 6
Chapter 6
Chapter 6
General Microbiology
CHAPTER 6
BASIC CONCEPTS OF
MEDICAL
PARASITOLOGY
Universities Press
3-6-747/1/A & 3-6-754/1, Himayatnagar
Hyderabad 500 029 (A.P.), India
Phone: 040-2766 5446/5447
Email: info@universitiespress.com
marketing@universitiespress.com
Dr Sonal Saxena, MD
Director Professor and Head of the Department of Microbiology
Maulana Azad Medical College,
New Delhi
and
Dr Amala A Andrews, MD
Maulana Azad Medical College,
New Delhi
PARASITOLOGY Multicellular: These can be seen with the naked eye, e.g.,
helminths such as roundworms
LIFE CYCLE OF Habitat: A place in which the parasite naturally lives and grows
PARASITES Host: An entity that provides nutrition and harbours the parasite
◦ Definitive host: This is a host in which the adult stage of the parasite
lives/undergoes sexual reproduction
◦ Intermediate host: A host that harbours the larval stage of the
parasite/in which asexual reproduction takes place
◦ Reservoir host: Harbours the parasite and also acts as a source of
infection
◦ Paratenic host: The parasite is unable to develop further in this host;
it can act as a transport or carrier host
(i) Protozoa
(ii) Stramenopila
(iii) Animalia
Immunological responses against parasites are lower than those against bacteria and viruses due to
the intracellular location of parasites and immunological tolerance
Premunition immunity: immunity which lasts only while the original infection is active
LABORATORY DIAGNOSIS
DISEASES iii) Zinc sulfate floatation: 33% zinc sulfate solution is used
iv) Formol ether sedimentation method: Ether and 10%
solution of formalin are used
3. Trichrome staining
Simple and rapid method
Used to visualise intestinal protozoa, human cells, yeasts and artifact material in the stool
Thick film
Fig. 6.9 Thick and thin films (Source: PHIL, Image ID 5905/ CDC)
ii) Monoxenic cultivation: The parasite is grown in the presence of a single species of associate
flora
iii) Axenic cultivation: The parasite is grown in the absence of any other metabolising cells
RPMI 1640 with human RBC in 7% carbon dioxide and 1–5% oxygen—used to
cultivate P. falciparum