INTRODUCTION TO

PERSONAL PROTECTIVE &

LAB SAFETY.
DR. S.S. WALVEKAR.

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 • Lab safety is first priority.

• Safety is the best analysis tool.

• Lab safety starts with me.

• Lab safety by choice not the chance.

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• The laboratory Safety begins with the recognition of all chemical
hazards.

• It is achieved through the application of common sense, a laboratory

safety-focused attitude.

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Hazards
• 1. Broken glassware.

• 2. corrosive reagents.

• 3. Mechanical hazards.

• 4. Poisonous flames that could be inhaled. FIRE

• 5. Gas leakages.

• 6. Electrical hazards.
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 Precautions against chemical hazards include:

• Corrosive chemicals should be appropriately labelled.

• Care must be taken when pouring reagents to avoid splashing.

• Chemicals for which there is little or conflicting information

about potential toxic effects should be treated as toxic.

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 Laboratory Coats

• The primary purpose of coats is used in the laboratory to protect against

splashes and spills.

• In the laboratory coats should be non-inflammable and easily removable.

• Gloves:-

• Gloves are required for routine laboratory practicals to protect the hands
when handling chemical, physical, or biological hazards t
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 Handling Glassware :-
• Breakage of glassware is a common cause of injuries in laboratories
works.

• Clean all glassware before using experimental laboratory work.

• Commonly used glassware are beakers, test tubes, conical flasks,

volumetric flasks, measuring cylinders, pipettes, and reagent bottles.

• Tests may lead to erroneous results if dirty glassware is used.

• Used glassware should be washed with water. 7

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 Electrical.
• Electrical hazards are commonly caused by faulty operation and
improper maintenance of electrical equipment.

• Results in minor burns to severe injuries that may lead to death.

• In the Laboratory all electrical equipment should be properly

grounded.

• Overload circuits should be avoided.

• Never to be used extension cord.

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• Electrical equipment should not be touched with wet hands.

• Do not neglect any laboratory any electrical safety features.

• Repair should be done by authorized persons.

• Do not leave equipment switched on when not in use

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• Work space:-

• Work space must be kept neat at all times, and before leaving the
laboratory all equipment and apparatus must be returned to their
shelf.

• Waste disposals:-

• After completion of the laboratory experiment unwanted material

must be discarded in designated containers. 11
• Remember a lab is a place for serious work!

•  Loose clothing should not be worn because it may dip into chemicals or fall
into a flame and catch fire Laboratory.

• Tie Back Loose Hair; One should not be engaged in conversation or other
disturbances.

• Hair can fall into the Bunsen burner and catch fire or can fall into a chemical
solution.

• Do not taste any chemicals! 12

 First Aid
• Skin burns should be washed under running water or ice water, and
petroleum jelly or burn ointment should be applied and then
covered with sterile gauze. Any blister formed must not be
punctured.

• Chemicals injury to the eyes must be treated by through washing

with water.

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• If accidentally swallowing chemicals, the mouth must be thoroughly
rinsed with water.

• Contamination with infected material in wounds caused by broken

glassware, must be thoroughly rinsed with water and washed with
soap solution before applying the antiseptic solution.

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• Protect Your Eyes
• Appropriate eye protection must be worn at all times!
• Wear shoes that cover your feet.
• Do not smell any chemicals directly!
• • If necessary to smell, use your hand to fan the vapor to your
nose. 
• Sharps must be disposed of in an approved sharps container.
• Sharps containers may never be placed in the normal waste.

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• Do not eat or drink in the lab.

• • Ways by which you can accidentally ingest harmful chemicals

Know the hazards of the materials being used Laboratory.

• Handle hot glassware with gloves or beaker with gloves.

• Heat test tubes at an angle, directing the opening oppositely to you.

• First, light the match, THEN Turn on the gas! Wash your hands with
soap and water before leaving Laboratory.  16
• Remove gloves before handling objects such as doorknobs,
telephones, pens, computer keyboards, pH meter or other
electronic buttons, or phones while in the lab.

• It might be convenient to have one gloved hand and one

ungloved hand to do procedures where these kinds of things are
used.
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• Never remove chemicals from the laboratory

• Don’t work alone in the lab You may need another person to
prevent injury or even save your life.

• Know how to dispose of waste.

• • Dispose of all waste materials according to the instructions.

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  Student Reactions in a Fire.

• Notify everyone in the room.

• If possible shut down any reaction in progress by removing the

heat/energy source.

• Proceed to the nearest exit and pull the nearest fire alarm.

• Evacuate the building.

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• Any incident, no matter how big or small it is, you must tell
teacher immediately.

• Always move sharp objects with sharp end down .

• Check cords and plugs for damage and keep the area dry when
working with electronics.

• Chemicals that touch the skin and eyes should be washed for
enough amount of time. 20
• Any liquid chemicals, never return the unused portions to
the original container.

• Wash your hands at the end of each lab work.

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The first aid kit.

• Cotton wool .

• Roller bandage.

• Scissors.

• Acetic acid.

• Milk of magnesia.
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• Milk of magnesia  helps to cause movement of the intestines.
This medication is also used to treat symptoms caused by too
much stomach acid such as heartburn, upset stomach, or
indigestion.

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• Mouth pipetting is
never allowed.
• Use of a
Micropipette is
recommended.

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