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Calibrating Eyepiece Graticule Using Micrometer / Calculating Linear Magnification
Calibrating Eyepiece Graticule Using Micrometer / Calculating Linear Magnification
GRATICULE USING
MICROMETER / CALCULATING
LINEAR MAGNIFICATION
A) 0.5 mm В) 10 μm С) 50 μm D) 100 μm B
The electronmicrograph shows a cell. MCQ 2
What is the actual diameter of the nucleus?
A) 0.6 μm
3.42 cm
B) 6 μm
C) 35 μm
D) 350 μm B
MCQ 3
The diagram shows a graduated slide, with divisions of 0.1 mm viewed using an eyepiece
graticule.
A
MCQ 4
D
MCQ 5
C
SQ 1
A student used a light microscope to view a specimen. They wanted to work out the actual size of the specimen, and decided
to use a stage micrometer, together with the ruler visible inside the eyepiece of the microscope.
a) Outline why a stage micrometer is needed as well as the eyepiece graticule. [2]
• The size of the units of the eyepiece graticule change depending on the magnification being used; [1 mark]
• The stage micrometer can be used to calibrate the graticule; [1 mark]
b) Describe the steps required to calibrate the eyepiece graticule of a light microscope using a stage micrometer. [3]
• Insert the eyepiece graticule and line up the scale of the graticule with the scale on the stage micrometer; [1 mark]
• Count the number of subdivisions of the stage micrometer that fit into each division of the eyepiece graticule; [1 mark]
• Multiply that number by the distance between each subdivision of the stage micrometer to give the distance between each division of the
eyepiece graticule; [1 mark]
c) Explain why the steps outlined in part b) would need to be repeated when the student changes to a more powerful objective
lens. [2]
c) Calculate the actual diameter, X–Y, of the mitochondrion labelled in Fig. 1.1.
Write down the formula that you will use to make your calculation. Give your answer to the nearest whole
nanometre (nm). [2]
Formula: correctly stated formula ; e.g. actual diameter = image length / magnification
Actual diameter: correct actual diameter of X–Y calculated from measurement from printed copy ;
A 213 nm (for 10 mm)
A 223 nm (for 10.5 mm)
A 234 nm (for 11 mm)
A 245 nm (for 11.5 mm)
A 255 nm (for 12 mm)
SQ 3
Fig. 1.1 is a transmission electron
micrograph of cells from duckweed,
Spirodela oligorrhiza.
SUMMARY
REFLECTION
Think about everything you know about estimating sizes of cells. What answers
would you give to the following questions?
• What is magnification?
• What is an eyepiece graticule?
• What is a stage micrometer?
• How do you calibrate an eyepiece graticule using a stage micrometer?