Questionnaire

On
Centrifugation and Chromatography

Submitted by,
R. Naga Deepika
1st BMB
22368052
A Pure Substance shows _______ spots on Chromatogram.

A) 0
B) 1
C) 2
D) 3
Answer: Option B (One)
A Pure substance produces One spot and an Impure substance
produces two or more spots.
In Gas Chromatography, zero air is used in which of the following
detectors?
A) Flame Ionization Detector
B) Electron Capture Detector
C) Photo Ionization Detector
D) Thermal Conductivity Detector
Answer: Option A
Flame Ionization Detector
Guard Column is normally used with which form of Chromatography?

A) Gel Permeation
B) Size exclusion
C) HPLC (High Performance Thin-layer Chromatography)
D) HPLC (High Performance Liquid Chromatography)
Answer: Option D
Four Proteins have same molar mass but differ in their mass to charge
ratio. The most effective technique you should choose to separate thes
proteins from a mixture would be?

A) Gel filtration
B) Ion- exchange Chromatography
C) Ultra filtration
D) Density Gradient Centrifugation
Answer: Option B
UV detector is used in one of the following methods.

A) Gas- liquid Chromatography

B) HPLC
C) Size exclusion Chromatography
D) Affinity Chromatography
Answer: Option B
Which of the following separation method is suited method for a Protein
sample with large differences in molecular mass?

A) Dialysis
B) Salting out process
C) Density Gradient Centrifugation
D) Rate Zonal Centrifugation
Answer: Option D
If the Centripetal force F is kept constant but the angular velocity is
doubled. The new radius of the path (original radius R) will be.

A) 2R
B) R/2
C) R/4
D) 4R
Answer: Option C
Explanation:
F= mw²R —> 1
Given,
F= m(2w)²R¹
F= m4w²R¹ —> 2
From 1&2
mw²R = m4w²R¹
R= 4R¹
R¹ = R/4
In Centrifugation, vertical bucket rotors are used most often for.

A) Differential pelleting of the particles

B) Gradient Centrifugation
C) Rapid sedimentation of coarse particles
D) None of the avove
Answer: Option B
Which of the following is used for sedimentation of RBC’s?
A) High speed Centrifuge
B) Low speed Centrifuge
C) Ultra Centrifuge
D) Vacuum Centrifuge
Answer: Option B
Which of the following Centrifugation is used to separate certain
organelles from whole cell?

A) Rate Zonal Centrifugation

B) Normal Centrifugation
C) Differential Centrifugation
D) Isopycnic Centrifugation
Answer: Option C