Multiparametric flow cytometry of

normal human bone marrow:

analysis and display strategies
GEIL-GTLLF

2008

Part one : Leukocyte subsets

A colour code is applied trhoughout this atlas:
Granulocytes in red
Monocytes in green
Lymphocytes in purple
All other cells, in a region of maturation defined by the exclusion of mature
cell types and thus dubbed « bermudes » in cyan
I.1
 Combinations
Tube 1 : CD11b/CD14/CD16/CD45 Tube 7 : HLADR/CD117/CD34/CD45
CD11b/CD14 p. I-5 HLADR/CD117 p. I.29
CD11b/CD16 p. I-6 DR/CD34 p. I.30
CD14/CD16 p. I-7 CD117/CD34 p. I.31
Tube 2 :CD11b/CD117/CD34/CD45 Tube 8 : CD34/CD56/CD19/CD45
CD11b/CD117 p. I-9 CD34/CD56 p. I.33
CD11b/CD34 p. I-10 CD34/CD19 p. I.34
CD117/CD34 p. I-11 CD56/CD19 p. I.35
Tube 3 :CD36/CD71/CD11c/CD45 Tube 9 : CD64/CD24/CD10/CD45
CD36/CD71 p. I-13 CD64/CD24 p. I.37
CD36/CD11c p. I-14 CD64/CD10 p. I.38
CD71/CD11c p. I-15 CD24/CD10 p. I.39
Tube 4 : CD34/CD13/CD33/CD45 Tube 10 : CD7/CD117/CD34/CD45
CD34/CD13 p. I-17 CD7/CD117 p. I.41
CD34/CD33 p. I.18 CD7/CD34 p. I.42
CD13/CD33 p. I.19 CD117/CD34 p. I.43
Tube 5 : CD38/HLADR/CD34/CD45 Tube 11 : CD22/CD10/CD20/CD45
CD38/HLADR p. I.21 CD22/CD10 p. I.45
CD38/CD34 p. I.22 CD22/CD20 p. I.46
HLADR/CD34 p. I.23 CD10/CD20 p. I.47
Tube 6 : CD38/CD10/CD19/CD45 Tube 12 : CD4/CD2/CD56/CD45
CD38/CD10 p. I.25 CD4/CD2 p. I.49
CD38/CD19 p. I.26 CD4/CD56 p. I.50
CD10/CD19 p. I.27 CD2/CD56 p. I.51

I.2
 Leukocyte subsets display

SSC
SSC

FLy
FSC CD45 FLx

FLy
FLy

FLy

FLx FLx FLx

I.3
 CD11b/CD14/CD16/CD45
TUBE 1

    Granulocytes Monocytes Lymphocytes Bermudes

GM B lineage
        B T NK
progenitors progenitors

CD11b/CD14 x x  x   x  

CD11b/CD16 x x x    x    

CD14/CD16 x x     x     

I.4
 SSC
SSC

FLy
CD11b/CD14
FSC CD45 FLx

FLy
FLy

FLy
FLx FLx FLx
Height

Height

CD14
SSC-

SSC-

PE
FSC-Height CD45APC CD11bFITC
CD14

CD14
CD14

PE

PE
PE

CD11bFITC CD11bFITC CD11bFITC

I.5
 SSC
SSC

FLy
CD11b/CD16
FSC CD45 FLx

FLy
FLy

FLy
FLx FLx FLx

CD16P
Height

Height
SSC-

SSC-

C7
FSC-Height CD45APC CD11bFITC
CD16P

CD16P

CD16P
C7

C7
C7

CD11bFITC CD11bFITC CD11bFITC

I.6
 SSC
SSC

FLy
CD14/CD16
FSC CD45 FLx

FLy
FLy

FLy
FLx FLx FLx

CD16P
Height

Height
SSC-

SSC-

C7
FSC-Height CD45APC CD14PE
CD16P

CD16P

CD16P
C7

C7
C7

CD14PE CD14PE CD14PE

I.7
 CD11b/CD117/CD34/CD45
TUBE 2

    Granulocytes Monocytes Lymphocytes Bermudes

GM B lineage
        B T NK
progenitors progenitors

CD11b/CD117 x x x   x  x  
CD11b/CD34 x  x x    x  x  
CD117/CD34 x  x        x  

I.8
 SSC
SSC

FLy
CD11b/CD117
FSC CD45 FLx

FLy
FLy

FLy
FLx FLx FLx

Height
Height

Height

SSC-
SSC-

SSC-

FSC-Height CD45APC CD45APC

CD117

CD117
CD117

PE

PE
PE

CD11bFITC CD11bFITC CD11bFITC

I.9
 SSC
SSC

FLy
CD11b/CD34
FSC CD45 FLx

FLy
FLy

FLy
FLx FLx FLx

CD34P
Height

Height
SSC-

SSC-

C5
FSC-Height CD45APC CD11bFITC
CD34P

CD34P

CD34P
C5

C5
C5

CD11bFITC CD11bFITC CD11bFITC

I.10
 SSC
SSC

FLy
CD117/CD34
FSC CD45 FLx

FLy
FLy

FLy
FLx FLx FLx

CD34P
Height

Height
SSC-

SSC-

C5
FSC-Height CD45APC CD117PE
CD34P

CD34P

CD34P
C5

C5
C5

CD117PE CD117PE CD117PE

I.11
 CD36/CD71/CD11c/CD45
TUBE 3

    Granulocytes Monocytes Lymphocytes Bermudes

GM B lineage
        B T NK
progenitors progenitors

CD 36/CD71 x x       x  
CD36/CD11c x  x        x  
CD71/CD11c x  x        x  

I.12
 SSC
SSC

FLy
CD36/CD71
FSC CD45 FLx

FLy
FLy

FLy
FLx FLx FLx
Height

Height

CD71
SSC-

SSC-

PE
FSC-Height CD45APC CD36FITC
CD71

CD71
CD71

PE

PE
PE

CD36FITC CD36FITC CD36FITC

I.13
 SSC
SSC

FLy
CD36/CD11c
FSC CD45 FLx

FLy
FLy

FLy
FLx FLx FLx

CD11cP
Height

Height
SSC-

SSC-

C5
FSC-Height CD45APC CD36FITC
CD11cP

CD11cP
CD11cP

C5

C5
C5

CD36FITC CD36FITC CD36FITC

I.14
 SSC
SSC

FLy
CD71/CD11c
FSC CD45 FLx

FLy
FLy

FLy
FLx FLx FLx

CD11cPC5
Height

Height
SSC-

SSC-

FSC-Height CD45APC CD71PE

CD11cPC

CD11cP

CD11cP
C5

C5
5

CD71PE CD71PE CD71PE

I.15
 CD34/CD13/CD33/CD45
TUBE 4

    Granulocytes Monocytes Lymphocytes Bermudes

GM B lineage
        B T NK
progenitors progenitors

CD34/CD13 x  x        x  

CD34/CD33 x x        x  

CD13/CD33 x x       x  

I.16
 SSC
SSC

FLy
CD34/CD13
FSC CD45 FLx

FLy
FLy

FLy
FLx FLx FLx
Height

Height

CD13
SSC-

SSC-

PE
FSC-Height CD45PC7 CD34FITC
CD13

CD13
CD13

PE

PE
PE

CD34FITC CD34FITC CD34FITC

I.17
 SSC
SSC

FLy
CD34/CD33
FSC CD45 FLx

FLy
FLy

FLy
FLx FLx FLx

CD33A
Height

Height
SSC-

SSC-

PC
FSC-Height CD45PC7 CD34FITC
CD33A

CD33A
CD33A

PC

PC
PC

CD34FITC CD34FITC CD34FITC

I.18
 SSC
SSC

FLy
CD13/CD33
FSC CD45 FLx

FLy
FLy

FLy
FLx FLx FLx

CD33A
Height

Height
SSC-

SSC-

PC
FSC-Height CD45PC7 CD13PE
CD33A

CD33A
CD33A

PC

PC
PC

CD13PE CD13PE CD13PE

I.19
 CD38/HLADR/CD34/CD45
TUBE 5

    Granulocytes Monocytes Lymphocytes Bermudes

GM B lineage
        B T NK
progenitors progenitors

CD38/DR x x       x x 
CD38/CD34 x          x x 
DR/CD34 x  x        x  

I.20
 SSC
SSC

FLy
CD38/HLADR
FSC CD45 FLx

FLy
FLy

FLy
FLx FLx FLx

HLADR
Height

Height
SSC-

SSC-

PE
FSC-Height CD45ECD CD38FITC
HLADR

HLADR
HLADR

PE

PE
PE

CD38FITC CD38FITC CD38FITC

I.21
 SSC
SSC

FLy
CD38/CD34
FSC CD45 FLx

FLy
FLy

FLy
FLx FLx FLx

CD34P
Height

Height
SSC-

SSC-

C5
FSC-Height CD45ECD CD38FITC
CD34P
CD34P

CD34P
C5

C5
C5

CD38FITC CD38FITC CD38FITC

I.22
 SSC
SSC

FLy
HLADR/CD34
FSC CD45 FLx

FLy
FLy

FLy
FLx FLx FLx

CD34P
Height

Height
SSC-

SSC-

C5
FSC-Height CD45ECD HLADRPE
CD34P
CD34P

CD34P
C5

C5
C5

HLADRPE HLADRPE HLADRPE

I.23
 CD38/CD10/CD19/CD45
TUBE 6

    Granulocytes Monocytes Lymphocytes Bermudes

GM B lineage
        B T NK
progenitors progenitors

CD38/CD10 x x       x x 
CD38/CD19    x x      x x 
CD10/CD19 x    x     x x 

I.24
 SSC
SSC

FLy
CD38/CD10
FSC CD45 FLx

FLy
FLy

FLy
FLx FLx FLx
Height

Height

CD10
SSC-

SSC-

PE
FSC-Height CD45Cy7 CD38FITC
CD10

CD10
CD10

PE

PE
PE

CD38FITC CD38FITC CD38FITC

I.25
 SSC
SSC

FLy
CD38/CD19
FSC CD45 FLx

FLy
FLy

FLy
FLx FLx FLx

CD19C
Height

Height
SSC-

SSC-

y5
FSC-Height CD45Cy7 CD38FITC
CD19C

CD19C

CD19C
y5

y5

y5
CD38FITC CD38FITC CD38FITC

I.26
 SSC
SSC

FLy
CD19/CD10
FSC CD45 FLx

FLy
FLy

FLy
FLx FLx FLx

CD19C
Height

Height
SSC-

SSC-

y5
FSC-Height CD45Cy7 CD10PE
CD19C

CD19C

CD19C
y5

y5

y5
CD10PE CD10PE CD10PE

I.27
 HLADR/CD117/CD34/CD45
TUBE 7

    Granulocytes Monocytes Lymphocytes Bermudes

GM B lineage
        B T NK
progenitors progenitors

DR/CD117 x x       x  
DR/CD34 x          x x 
CD117/CD34  x x        x

I.28
 SSC
SSC

FLy
HLADR/CD117
FSC CD45 FLx

FLy
FLy

FLy
FLx FLx FLx

CD117
Height

Height
SSC-

SSC-

PE
FSC-Height CD45Cy7 HLADRFITC
CD117

CD117
CD117

PE

PE
PE

HLADRFITC HLADRFITC HLADRFITC

I.29
 SSC
SSC

FLy
HLADR/CD34
FSC CD45 FLx

FLy
FLy

FLy
FLx FLx FLx

CD34C
Height

Height
SSC-

SSC-

y5
FSC-Height CD45Cy7 HLADRFITC
CD34C

CD34C

CD34C
y5

y5

y5
HLADRFITC HLADRFITC HLADRFITC

I.30
 SSC
SSC

FLy
CD117/CD34
FSC CD45 FLx

FLy
FLy

FLy
FLx FLx FLx

CD34C
Height

Height
SSC-

SSC-

y5
FSC-Height CD45Cy7 CD117PE
CD34C

CD34C

CD34C
y5

y5

y5
CD117PE CD117PE CD117PE

I.31
 CD34/CD56/CD19/CD45
TUBE 8

    Granulocytes Monocytes Lymphocytes Bermudes

GM B lineage
        B T NK
progenitors progenitors

CD34/CD56     x x  x
CD34/CD19      x     x x 
CD56/CD19    x   x  x 

I.32
 SSC
SSC

FLy
CD34/CD56
FSC CD45 FLx

FLy
FLy

FLy
FLx FLx FLx
Height

Height

CD56
SSC-

SSC-

PE
FSC-Height CD45APC CD34FITC
CD56

CD56
CD56

PE

PE
PE

CD34FITC CD34FITC CD34FITC

I.33
 SSC
SSC

FLy
CD34/CD19
FSC CD45 FLx

FLy
FLy

FLy
FLx FLx FLx

CD19P
Height
Height
SSC-

SSC-

C7
FSC-Height CD45APC CD34FITC
CD19P

CD19P
CD19P

C7

C7
C7

CD34FITC CD34FITC CD34FITC

I.34
 SSC
SSC

FLy
CD56/CD19
FSC CD45 FLx

FLy
FLy

FLy
FLx FLx FLx

CD19P
Height

Height
SSC-

SSC-

C7
FSC-Height CD45APC CD56PE
CD19P

CD19P
CD19P

C7

C7
C7

CD56PE CD56PE CD56PE

I.35
 CD64/CD24/CD10/CD45
TUBE 9

    Granulocytes Monocytes Lymphocytes Bermudes

GM B lineage
        B T NK
progenitors progenitors

CD64/CD24 x x  x     x  x
CD64/CD10  x x        x x 
CD24CD10  x x      x x 

I.36
 SSC
SSC

FLy
CD64/CD24
FSC CD45 FLx

FLy
FLy

FLy
FLx FLx FLx
Height

Height

CD24
SSC-

SSC-

PE
FSC-Height CD45Cy7 CD64FITC
CD24

CD24
CD24

PE

PE
PE

CD64FITC CD64FITC CD64FITC

I.37
 SSC
SSC

FLy
CD64/CD10
FSC CD45 FLx

FLy
FLy

FLy
FLx FLx FLx

CD10C
Height

Height
SSC-

SSC-

y5
FSC-Height CD45Cy7 CD64FITC
CD10C

CD10C

CD10C
y5

y5

y5
CD64FITC CD64FITC CD64FITC

I.38
 SSC
SSC

FLy
CD24/CD10
FSC CD45 FLx

FLy
FLy

FLy
FLx FLx FLx

CD10C
Height

Height
SSC-

SSC-

y5
FSC-Height CD45Cy7 CD24PE
CD10C

CD10C

CD10C
y5

y5

y5
CD24PE CD24PE CD24PE

I.39
 CD7/CD117/CD34/CD45
TUBE 10

    Granulocytes Monocytes Lymphocytes Bermudes

GM B lineage
        B T NK
progenitors progenitors

CD7/CD117 x   x    x  
CD7/CD34       x    x  

CD117/CD34  x         x

I.40
 SSC
SSC

FLy
CD7/CD117
FSC CD45 FLx

FLy
FLy

FLy
FLx FLx FLx

CD117
Height

Height
SSC-

SSC-

PE
FSC-Height CD45Cy7 CD7FITC
CD117

CD117
CD117

PE

PE
PE

CD7FITC CD7FITC CD7FITC

I.41
 SSC
SSC

FLy
CD7/CD34
FSC CD45 FLx

FLy
FLy

FLy
FLx FLx FLx

CD34C
Height

Height
SSC-

SSC-

y5
FSC-Height CD45Cy7 CD7FITC
CD34C

CD34C
CD34C

y5

y5
y5

CD7FITC CD7FITC CD7FITC

I.42
 SSC
SSC

FLy
CD117/CD34
FSC CD45 FLx

FLy
FLy

FLy
FLx FLx FLx

CD34C
Height

Height
SSC-

SSC-

y5
FSC-Height CD45Cy7 CD117PE
CD34C

CD34C
CD34C

y5

y5
y5

CD117PE CD117PE CD117PE

I.43
 CD22/CD10/CD20/CD45
TUBE 11

    Granulocytes Monocytes Lymphocytes Bermudes

GM B lineage
        B T NK
progenitors progenitors

CD22/CD10 x     x
CD22/CD20     x      x 
CD10/CD20   x      x 

I.44
 SSC
SSC

FLy
CD22/CD10
FSC CD45 FLx

FLy
FLy

FLy
FLx FLx FLx
Height

Height

CD10
SSC-

SSC-

PE
FSC-Height CD45PC7 CD22FITC
CD10

CD10
CD10

PE

PE
PE

CD22FITC CD22FITC CD22FITC

I.45
 SSC
SSC

FLy
CD22/CD20
FSC CD45 FLx

FLy
FLy

FLy
FLx FLx FLx

CD20P
Height

Height
SSC-

SSC-

C5
FSC-Height CD45PC7 CD22FITC
CD20P

CD20P
CD20P

C5

C5
C5

CD22FITC CD22FITC CD22FITC

I.46
 SSC
SSC

FLy
CD10/CD20
FSC CD45 FLx

FLy
FLy

FLy
FLx FLx FLx

CD20P
Height

Height
SSC-

SSC-

C5
FSC-Height CD45PC7 CD10PE
CD20P

CD20P
CD20P

C5

C5
C5

CD10PE CD10PE CD10PE

I.47
 CD4/CD2/CD56/CD45
TUBE 12

    Granulocytes Monocytes Lymphocytes Bermudes

GM B lineage
        B T NK
progenitors progenitors

CD4/CD2   x  x   

CD4/CD56       x  x   

CD2/CD56     x x   

I.48
 SSC
SSC

FLy
CD4/CD2
FSC CD45 FLx

FLy
FLy

FLy
FLx FLx FLx
Height

Height
SSC-

SSC-

CD2
PE
FSC-Height CD45APC CD4FITC
CD2

CD2

CD2
PE

PE
PE

CD4FITC CD4FITC CD4FITC

I.49
 SSC
SSC

FLy
CD4/CD56
FSC CD45 FLx

FLy
FLy

FLy
FLx FLx FLx

CD56P
Height

Height
SSC-

SSC-

C7
FSC-Height CD45APC CD4FITC
CD56P

CD56P
CD56P
C7

C7

C7
CD4FITC CD4FITC CD4FITC

I.50
 SSC
SSC

FLy
CD2/CD56
FSC CD45 FLx

FLy
FLy

FLy
FLx FLx FLx

CD56P
Height

Height
SSC-

SSC-

C7
FSC-Height CD45APC CD2PE
CD56P

CD56P
CD56P
C7

C7

C7
CD2PE CD2PE CD2PE

I.51
 Published on ELN website
July 2008
• Contributors
– Christine Arnoulet
– Marie Christine Béné
– Françoise Durrieu
– Jean Feuillard
– Chantal Fossat
– Bernard Husson
– Hélène Jouault
– Francis Lacombe
– Marc Maynadié
• Coordination
– Francis Lacombe & Marie Christine Béné