Lesson objective: To describe gram staining principle for classification of microorganisms and interpret and draw

pie charts.
KEYWORDS: pie chart, gram staining, gram positive, gram negative

All students will

All:
be able to
describe gram
staining
principle.
SUBJECT: SCIENCE
TEACHER: Ms. JIBI ELIZABETH
Most:
Most students YEAR: 9
will be able to
TOPIC: UNICELLULAR ORGANISMS
realize
classification of
bacteria.

Some:
Some students
will be able to
interpret and
draw pie charts.
 Lesson objective: To describe gram staining principle for classification of microorganisms and interpret and draw
pie charts.
KEYWORDS: pie chart, gram staining, gram positive, gram negative
MY HPL JOURNEY
All students will
All: Advanced Cognitive Performance Characteristics (ACPS)
be able to Values Attitudes and Attributes (VAAs)
Meta thinking: Have you
describe gram1. Evaluated your own learning? Creating: Have you
1. Given an example of new ideas?
staining 2. Done self-corrections? 2. Abandoned one idea for another as you Empathetic:
3. Approached new content by linking it to your realized it didn’t work?
principle. current knowledge? 3. Created a new idea by building on ones
1. When have you collaborated during the course of your
topic?
4. Given your personal views on a matter based you already have? 2. How would this topic affect society and the community?
on evidence? 4. You can take a picture or write about it in 3. What moral or ethical issues were raised during the topic?
5. Transferred knowledge you already have to a your notebook. 4. When did you feel under stress during this topic but
Most:
Most students new circumstance? gained the confidence in your knowledge?
will be able to
Analysing: Have you Agile:
realize 1. When were you willing to work alone?
classification of 1. Broken down your task and decided what the best approach to take would be?
2. Written a Hypothesis?
2. When did you think independently?
3. When did you actively control your own learning?
bacteria. 3. Followed a set of rules within science? (not including class rules).
4. When did you use your initiative to find solutions?
4. You can write about it in your notebook.
5. When did you take on the views or ideas of others?

Some: Linking: Have you Hardworking:

Some students1. Taken on the views of others? 1. How did you overcome the obstacles and difficulties you
will be able to 2. Connected your past learning to new faced during your learning journey?
2. How did you train yourself to move forward in adverse
interpret and 3. learning?
Taken what you have learnt now and conditions?
draw pie charts. applied it to a new situation?
 Lesson objective: To describe gram staining principle for classification of microorganisms and interpret and draw
pie charts.
KEYWORDS: pie chart, gram staining, gram positive, gram negative

All students will

All:
be able to STARTER:
describe gram
staining
principle. Write down the names of few bacteria
and their functions.
Most:
Most students
will be able to
realize
classification of
bacteria.

Some:
Some students
will be able to
interpret and
draw pie charts.
 Lesson objective: To describe gram staining principle for classification of microorganisms and interpret and draw
pie charts.
KEYWORDS: pie chart, gram staining, gram positive, gram negative

All students will

All:
be able to GRAM STAINING:
describe gram This test differentiate the bacteria into Gram Positive and
staining
principle. Gram-Negative Bacteria, which helps in the classification
and differentiations of microorganisms.
Principle of Gram Staining
Most:
Most students
will be able to
When the bacteria is stained with primary stain Crystal
realize Violet and fixed by the mordant, some of the bacteria are
classification of able to retain the primary stain and some are decolorized
bacteria.
by alcohol.
The ethanol cannot remove the Crystal Violet-Iodine
Some:
Some students complex that is bound to the thick layer of peptidoglycan
will be able to
interpret and of gram-positive bacteria and appears blue or purple in
draw pie charts. colour.
 Lesson objective: To describe gram staining principle for classification of microorganisms and interpret and draw
pie charts.
KEYWORDS: pie chart, gram staining, gram positive, gram negative

PROCEDURE OF GRAM STAINING

All students will
All:
be able to
1.Take a clean, grease free slide.
describe gram 2.Prepare the smear of suspension on the clean slide with a loopful
staining of sample.
principle.
3.Air dry and heat fix
4.Crystal Violet was poured and kept for about 30 seconds to 1
Most:
Most students minutes and rinse with water.
will be able to 5.Flood the gram’s iodine for 1 minute and wash with water.
realize
classification of 6.Then ,wash with 95% alcohol or acetone for about 10-20 seconds
bacteria. and rinse with water.
7.Add safranin  for about 1 minute and wash with water.
Some:
Some students 8.Air dry, Blot dry and Observe under Microscope.
will be able to
interpret and
draw pie charts.
 Lesson objective: To describe gram staining principle for classification of microorganisms and interpret and draw
pie charts.
KEYWORDS: pie chart, gram staining, gram positive, gram negative

All students will

All:
be able to INTERPRETATION:
Gram Positive: Blue/Purple Color
describe gram Gram Negative: Red Color
staining
principle.

Most:
Most students
will be able to
realize Gram Positive Bacteria: Actinomyces, Bacillus, Clostridium, Corynebacterium,
classification of Enterococcus, Gardnerella, Lactobacillus, Listeria, Mycoplasma, Nocardia,
bacteria.
Staphylococcus, Streptococcus, Streptomyces ,etc.
Gram Negative Bacteria: Escherichia coli (E. coli), Salmonella, Shigella, and other
Some:
Some students Enterobacteriaceae, Pseudomonas,Moraxella, Helicobacter, Stenotrophomonas, B
will be able to dellovibrio, acetic acid bacteria, Legionella etc
interpret and
draw pie charts. ANIMATION OF GRAM STAINING.
 Lesson objective: To describe gram staining principle for classification of microorganisms and interpret and draw
pie charts.
KEYWORDS: pie chart, gram staining, gram positive, gram negative

All students will

All:
be able to
describe gram PROGRESS CHECK:
staining
principle.
Answer the following:
1. Explain the principle of ‘Gram
Most:
Most students
staining’’.
will be able to 2. Give examples of ‘’Gram-positive’’ and
realize
classification of ‘’Gram-Negative’’ bacteria.
bacteria.

Some:
Some students
will be able to
interpret and
draw pie charts.
 Lesson objective: To describe gram staining principle for classification of microorganisms and interpret and draw
pie charts.
KEYWORDS: pie chart, gram staining, gram positive, gram negative

All students will

All: DRAWING PIE CHARTS:
be able to
Pie charts used to show something to
describe gram
staining whole. The different sectors of pie chart are
principle. drawn using angle. A bigger angle gives
bigger sector.
Most:
Most students
will be able to
realize
classification of
bacteria.

Some:
Some students
will be able to
interpret and
draw pie charts.
 Lesson objective: To describe gram staining principle for classification of microorganisms and interpret and draw
pie charts.
KEYWORDS: pie chart, gram staining, gram positive, gram negative

All students will

All:
STEPS FOR DRAWING PIE CHARTS:
be able to
describe gram
staining
principle.

Most:
Most students
will be able to
realize
classification of
bacteria.

Some:
Some students
will be able to
interpret and
draw pie charts.
 Lesson objective: To describe gram staining principle for classification of microorganisms and interpret and draw
pie charts.
KEYWORDS: pie chart, gram staining, gram positive, gram negative

All students will

All:
be able to
describe gram
staining
principle.

Most:
Most students
will be able to
realize
classification of
bacteria.

Some:
Some students
will be able to
interpret and
draw pie charts.
 Lesson objective: To describe gram staining principle for classification of microorganisms and interpret and draw
pie charts.
KEYWORDS: pie chart, gram staining, gram positive, gram negative

All students will

PROGRESS CHECK:
All:
be able to
describe gram
staining
principle.

Most:
Most students
will be able to
realize
classification of
bacteria.

Some:
Some students
will be able to
interpret and
draw pie charts.
 Lesson objective: To describe gram staining principle for classification of microorganisms and interpret and draw
pie charts.
KEYWORDS: pie chart, gram staining, gram positive, gram negative

All students will

All:
be able to
ANSWER KEY:
describe gram
staining
principle.

Most:
Most students
will be able to
realize
classification of
bacteria.

Some:
Some students
will be able to
interpret and
draw pie charts.
 Lesson objective: To describe gram staining principle for classification of microorganisms and interpret and draw
pie charts.
KEYWORDS: pie chart, gram staining, gram positive, gram negative

All students will

All:
be able to
describe gram
staining
principle.

Most:
Most students 1. HPL used.
will be able to 2.VAA
realize 3.ACP
classification of
bacteria.

Some:
Some students
will be able to
interpret and
draw pie charts.