THE ORGANOID FACILITY

Department of Radiation Sciences; Wallenberg Centre for

Molecular Medicine at Umeå University

Introduction:
Organoids are advanced 3-dimensional tissue cultures grown in vitro. They represent simplified version of the organs from which they are derived. Organoid
cultures can replicate complexity of an organ under specific growth conditions. They have application in pre-clinical research, drug discovery, and precision
medicine, especially in cancer patients. Organoids can be grown from most organs including brain, heart, kidney, lungs, and the organs of the
gastrointestinal tract such as liver, pancreas, intestine, etc. We specialize in establishing and maintaining organoids from resected pancreas of cancer
patients, and the mouse model.

Pancreatic Organoids Facility

Location:
TRC building, 6M, 3rd Floor; Room #5017

Contact:
Mitesh Dongre
mitesh.dongre@umu.se Daniel Öhlund,
Head of the facility

What we offer:
 Matrigel Shop:
Supply matrigel for 3D-cell culture experiments

 Production and supply of conditioned media

containing desired growth factors:
R-Spondin
Wnt3a

Primary tumor, malignant,
or healthy pancreas
from patient or animal model
~2 weeks
 Technical support:
Information and resources required for establishing organoids
 Hands-on training in organoid culture (On demand):
Establish and maintain human and murine pancreatic organoids

Infrastructure:
Organoids

 Organoid-culture lab: Cutting edge cell culture facility

Pancreatic
Establish organoids
duct

 Spectramax I3:
Multimode detection
platform

 Evos Microscope:
Genetic Live-cell microscopy
Comparative Omics Multiple cell type
modification; approaches and (Co-culture) (Brightfield, GFP,
CRISPR/Cas9, Lentivirus,
sequencing etc. models mCherry)
Adenovirus