IDENTIFICATION OF PROTEINS: NINHYDRIN TEST

Group 2B Ladrillono, Mercie Basco, Pamela Bautista, Syd Florence Ocampo, Shelly Anne Rubio, Raenel Tecson, John Albert Torres, Mara Isabel Uy, Miko Mariel

NINHYDRIN TEST
Test

for amino acids and proteins with a free NH2 group When an -NH2 group reacts with ninhydrin (0.1% triketohydrindene hydrate), a purple-blue complex is formed

OVER-ALL REACTION:

MECHANISM:

 Amines

(including -amino acids) react with ninhydrin to give a colored product.

-amino acids typically give a blue-purple product (positive result). [Eq. 2] a secondary amine, gives a yellow-orange colored solution as a condensation product. [Eq. 3]

The

Proline,

 It

can be used qualitatively (e.g. for chromatographic visualization) or quantitatively (e.g. for peptide sequencing).
test is sensitive enough that ninhydrin can be used for the visualization of fingerprints.

The

PROCEDURE:
1 mL diluted plasma was put in a test tube and added with 0.5 mL of ninhydrin reagent. Solution was mixed, heated for 1 minute and allowed to cool. Colored solution formed was noted.
Ninhydrin test was repeated using 1 mL of 0.3% soution of glycine (positive control) instead of plasma. Color obtained was compared to the diluted plasma.

RESULTS:

Diluted Plasma

Light Purple Solution

Positive Control: Glycine

Blue-Violet Solution

Negative Control: Water Clear Solution