Compact SPR Biosensor

CCD camera
Laser or or photo diode
LED array

cover
plate display

disposable sensor chip

polarizer prism lens

sample cassette digital window sample holder
 Self-referencing SPR

• For the sample with low concentration, signal shift might

be smaller than environmental drift change.
• Self-referencing eliminates influence by environment
(excellent robustness against temperature and buffer
change).
Top view
PDMS flow cell

analyte

z
substrate
y
x
 Experimental procedure
Step 1 Top view
PDMS micro
flow cell
solution

Side view
substrate
Au
width: 50 – 300 μm for channels glass slide
and spaces between channels

Step 2 Step 3 channel width:

500 μm – 1 cm
PDMS flow cell

Au
z
glass slide
substrate y
x
Multi-step functionalization procedure using
mouse IgG as reference
mouse IgG

OH OH
O O

MUA antibody
Au
S S S S S S S

Au
glass slide glass slide

sensing referencing
area area

OH OH
O O

OH OH
O O

S S S S S S S

antigen
Au
S S S S S S S

Au
glass slide glass slide

Taya, figs for pat appl, Aug 2005, fr Fengyu REV Aug 30.ppt
 Mouse IgG absorption onto the gold surface
(a) (c)

(b)
(d)
y axis of sensor chip

pbs

mouse IgG 100 g/ml

image pixel number

 Anti-CEA antibodies immobilized onto sensor chip
(a)
(c)

(b)
(d)
y axis of sensor chip

image pixel number

Sensorgrams of TKH2 antibodies immobilizing onto sensor
chip and STn antigens binding to TKH2 antibodies

(a) (b)
sensing (1)
sensing (1)
referencing (2) referencing (2)

(1) - (2)
(1) - (2)

TKH2 20 g/ml STn 100 ng/ml

Sensorgrams of anti-CEA antibodies immobilizing onto sensor
chip and CEA antigens binding to antibodies

(a) (b)
sensing (1)
sensing (1)
referencing (2)
referencing (2)

(1) - (2)
(1) - (2)

anti-CEA antibody 20 g/ml CEA 10 ng/ml