The document discusses bioleaching experiments to recover metals from waste printed circuit boards (WPCBs). Bacteria were cultured in an inorganic medium and used to inoculate spent medium containing added sulfur. For direct bioleaching, raw WPCBs were added once the pH dropped below 0.8. For indirect bioleaching, treated WPCBs were added to the spent medium at 2.5% pulp density. Metal recovery from raw WPCBs was tested under different direct and indirect bioleaching systems.
The document discusses bioleaching experiments to recover metals from waste printed circuit boards (WPCBs). Bacteria were cultured in an inorganic medium and used to inoculate spent medium containing added sulfur. For direct bioleaching, raw WPCBs were added once the pH dropped below 0.8. For indirect bioleaching, treated WPCBs were added to the spent medium at 2.5% pulp density. Metal recovery from raw WPCBs was tested under different direct and indirect bioleaching systems.
The document discusses bioleaching experiments to recover metals from waste printed circuit boards (WPCBs). Bacteria were cultured in an inorganic medium and used to inoculate spent medium containing added sulfur. For direct bioleaching, raw WPCBs were added once the pH dropped below 0.8. For indirect bioleaching, treated WPCBs were added to the spent medium at 2.5% pulp density. Metal recovery from raw WPCBs was tested under different direct and indirect bioleaching systems.
Valentina Bermúdez Gómez – 1001013365 THE BACTERIA WERE CULTURED IN THE INORGANIC MEDIUM CONTAINING 1.0 G/L (NH4)2SO4, 1.0 G/L KNO3, 1.0 G/L
Microorganisms KH2PO4, 0.5 G/L MGSO4•7H2O
AND 0.25 G/L CACL2, AND HAD A NATURAL OF PH 5.0. F
and culture THE PURE CULTURE OF CELLS
WAS THEN HARVESTED BY CENTRIFUGATION (8000 RPM, 10 conditions MIN) AND WASHED THREE TIMES USING STERILE NORMAL SALINE (PH=7.0) TO REMOVE THE NUTRIENT MATTERS. Direct and indirect Bioleaching experiments bioleaching With the spent medium in which For direct bioleaching, when the pH of the 8g/l of sulfur is added to the bioleaching media decreased to 0.8 after inorganic salt medium and then 3 culture, the raw WPCBs were added into the inocula are inoculated, then 100 ml media. For indirect bioleaching and for the of the medium are incubated in a indirect were added into the spent medium at a shaker and after 10 days that the pulp density of 2.5% (w/v). Different amounts pH drops, it is centrifuged at 8000 (2.5%, 5%, 7.5%, and 10%) of the 600 ◦C- rpm for 10 minutes and thus treated WPCBs were added into the spent eliminate bacteria medium.
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Metal recovery from the raw WPCBs under different leaching systems