Aga khan education service Pakistan

South region
Digital learning mode
Biology academic year 2020-21
3.3 Mechanism of
Enzyme Action
 3.3.1 compare lock and key
model and induced fit model of
enzyme action;

3.3.2 illustrate the mechanism

of enzyme action through lock
and key model and induced fit
model;
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 Lock and Key model

 Proposed by Emil fischer in 1894.

 Only the correctly shaped key opens a particular lock.
 Active site is not flexible and have fixed geometrical shape. 
 It does not tell the stabilization state of enzyme.

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Samreen Arain Ch#3
 Induced fit model:

 Proposed by Daniel Koshland in 1958.

 The active site continues to change until the substrate is completely
bound to the active site of the enzyme, at which point the final shape
and charge is determined.
 Active site is flexible.
 It explains the stabilization of enzyme.

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3.3.3 define energy of activation;

 Activation energy, in chemistry, the minimum amount

of energy that is required to activate atoms or
molecules to a condition in which they can undergo
chemical reaction.

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3.3.4 explain that enzymes speed up a
chemical reaction by lowering the
energy of activation using graphs;

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 Enzymes lower the activation energy to a point where a small
amount of available heat can push the reactants to a transition
state.
 Straining the reactants and bringing them close together are
two common ways the enzymes use to lower the activation
energy.
 The thermal energy speeds up the motion of the reactant
molecules, increasing the frequency and force of their
collisions, and also the atoms and bonds within the individual
molecules, making it more likely that bonds will break.

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