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Using DNA Technology To Produce Transgenic Animals
Using DNA Technology To Produce Transgenic Animals
Transgenic Animals
I. Introduction
Transgenic animals:
Animals which have been genetically engineered to contain one or more genes from an exogenous source. Transgenes are integrated into the genome.
required.
Mated on day 3 Fertilized oocytes microinjected on day 4 with foreign DNA construct. Microinjected oocytes are transferred to the oviducts of surrogate mothers at end of day 4.
injected gene from those of an endogenous counterpart, the injected gene must be marked in some way.
Mini-genes where exons are deleted of cDNA where introns are absent. Modification by insertion/deletion/mutagenesis of a few nucleotides (e.g. the gain or loss of a restriction endonuclease site). Hybrid genes where foreign epitopes are expressed on transgenic products.
it is expressed appropriately, despite the fact that it has integrated at a different chromosomal location.
CfTCR in goats
Tissue plasminogen
pigs Human lactoferrin in cows milk Alpha-1-antitrypsin in sheep HGH in mouse urine (uroplakin promoters) Human antibodies in mice (H and L chain tgenics hybridomas)
activator (TPA) in goats Human antithrombin III in goats Malaria antigens in goats (vaccine) Alpha-glucosidase in rabbits (Pompes disease
human complement is activated and triggers the complement cascade and organ destruction.
Transgenic pigs with complement inhibitors have
been produced and are gaining feasibility as a source of xenogeneic organs for transplantation.