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Bacteriology Lab Second Class F
Bacteriology Lab Second Class F
Department of Microbiology
College of Medicine
University of Baghdad
2015-2016
Learning Objectives
• Understand why specimen collection is Important in
Microbiology
• List types of specimens for microbiology lab
• List types of differential stains
• Define culture media
• Identify the basic ingredients of culture media
• List types of culture media
• Explain the laboratory identification methods of
bacteria
Specimen collection
Why specimen collection is Important in Microbiology
• Specimen collection in Microbiology to isolate and
identify the causative agents forms back bone of the
investigative procedures.
• In developing world, lack of awareness and casual
attitude among junior staff hampers the definitive
diagnosis.
• Specific procedures in collecting specimens will certainly
improve the quality of services of Microbiology
Departments
Preparation Smear
What is Smear?
A specimen for microscopic study prepared by
spreading the material across the glass slide.
What is Culture ?
Propagation of microorganisms in a growth media.
What is Growth media?
An artificial media contains basic requirements
needed for microorganisms' growth.
We have 2 main forms of growth media :
Growth media
•Liquid or called (broth) usually put it in test tube.
Sterile Broth
COLONY
Sterile
Petri
Staining of Bacteria
Bacteria cells are almost colorless and transparent
6
Types of staining techniques
For visualization of
morphological Identificatio Visualization
shape & arrangement. n of structure
Gram Acid fast
stain stain Spore Capsule
stain stain
7
Shapes of Bacteria
Culture Media
An artificial media contains basic requirements
needed for microorganisms' growth.
-Why differential?
Bec. it differentiate
bet. Hemolytic &
non hemolytic bac.
Transport media
sugar media
Pure Culture
It is a single kind of microorganisms growing alone in
a protected environment.
Used for the preparation of pure cultures from
laboratory specimens containing mixed
microorganisms to easily dx.
Media and equipment to be used must be sterile. and
Technique must be aseptic.
Cultural characteristics
Slide Tube
method method
Biochemical tests for the isolated
microorganism
• Detect of enzyme activity: Example
2. Catalase test : Inactivates H2O2.
Antimicrobial activity of microorganism