eCL8000 Installation Guide

eCL8000
Installation
Introduction
CONTENTS
1 Product Principle
2 Reagents
3
1 Installation Requirement Condition
3
1
4 Preparation for Installation
5
1 Product Installation
16 Product Maintenance
PART 01
Product principle
Product Principle - Sandwich
Antigen with more
epitope
Ruthenium-labeled
antibody
Biotinylated antibody
Streptavidin-coated
micro bead
TPA Tripropylamine
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Product Principle - Sandwich Assay
Antigen with more
epitope
Ruthenium-labeled
antibody
Streptavidin-coated
micro bead
TPA Tripropylamine
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Antigen with more
epitope
Ruthenium-labeled
antibody
TPA
Streptavidin-coated
micro bead
TPA Tripropylamine
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Step 1: Let macromolecular antigen (Ag) in the sample to be determined, biotin-labeled antibody
(Btd-Ab) and luminescent-marker-labeled antibody (Ru-Ab) react in one assay cup. After
incubation for a while, sandwich immune complexes of (Btd-Ab)-Ag-(Ru-Ab) will be formed;
Step 2: Add streptavidin-coated magnetic beads (M); after incubation for a period, magnetic bead
immune complexes of M-(Btd-Ab)-Ag-(Ru-Ab) will be formed;
Step 3: Transfer the reaction product of Step 2 to the measuring cell; magnetic bead immune
complexes will be adsorbed onto the working electrode by the magnet; meanwhile, rinse the
electrode with TPA buffer solution to remove unbound sample and reagent;
Step 4: Remove the magnet, apply voltage to the electrode to start ECL reaction and record
electrochemical signals. As can be seen, when the double antibody sandwich method is used in the
reaction system of the device, luminescence intensity increases as more Ag in the sample is bound
to Ru-Ab. Therefore, the ECL intensity is directly proportional to the concentration of antigen in the
sample to be tested
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Product Principle - Competitive Assay
Antigen with less
epitope
Ruthenium-labeled
antibody
Biotinylated antigen
Streptavidin-coated
micro bead
TPA Tripropylamine
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Antigen with less
epitope
Ruthenium-labeled
antibody
Streptavidin-coated
micro bead
TPA Tripropylamine
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Antigen with less
epitope
Ruthenium-labeled
antibody
TPA
Streptavidin-coated
micro bead
TPA Tripropylamine
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Step 1: Let macromolecular antigen (Ag) in the sample to be determined and luminescent-marker-
labeled antibody (Ru-Ab) react in one assay cup and incubate for a period;
Step 2: Add biotin-labeled antigen (Btd-Ag) and streptavidin-coated magnetic beads (M); now antigen
(Ag) in the sample and Btd-Ag will be competitively bound to Ru-Ab. As a result of the specific affinity
interaction between biotin and streptavidin, biotin will be specifically bound to streptavidin, forming
magnetic bead immune complexes of M-(Btd-Ag)-(Ru-Ab); incubate for a while;
Step 3: Transfer the reaction product of Step 2 to the measuring cell; magnetic bead immune complexes
will be adsorbed onto the working electrode by the magnet; meanwhile, rinse the electrode with TPA
buffer solution to remove unbound sample and reagent;
Step 4: Remove the magnet; apply voltage to the electrode to start ECL reaction; record electrochemical
signals. As can be seen, when competitive assay is used in the ECLIA reaction system, Ag in the sample
to be determined and Btd-Ag are competitively bound to Ru-Ab, whereas only Btd-Ag can be bound to
M and therefore attracted and fixed by the magnet, forming the complexes of M-(Btd-Ag)-(Ru-Ab).
Then the complexes are involved in TPA excited ECL reaction; higher luminescence intensity indicates
that Btd-Ag is competitively bound to more antibodies and that Ag in the sample to be determined is
bound to fewer antibodies. Therefore, the ECL intensity is inversely proportional to the concentration of
antigen in the sample to be determined.
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Product Principle - Luminous Principle
Ru(bpy)2+ e-
Tripyridine Ru(bpy)3+ Ru(bpy)2+

Ruthium
Voltage e-
TPA*
Buffer TPA e-
H+
Ru(bpy)2+
Tripropylamine
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Product Principle - Luminous Principle
ECL Test Method
In chemiluminescence immunoassay, after the antibodies and the analyte form immune complexes
on the surface of magnetic beads, the complexes are carried by buffer solution into the measuring
cell. Immune complexes formed on the surface of magnetic beads will be adsorbed by the magnet
below the measuring cell to the electrode surface; immune complexes failing to bind magnetic
beads cannot be adsorbed to the electrode surface, and are directly flushed away from the electrical
excitation zone by buffer solution. Upon applying excitation voltage, tris (2,2’-bipyridine)
ruthenium(II) in excited state will emitted photons at 620 nm wavelength. The intensity of optical
signals detected by the photomultiplier tube above the measuring cell is correlated with the amount
of analyte captured on the surface of magnetic beads, thus quantitative analysis of the analyte can
be realized. After the test, flow cleaning solution into the measuring cell to clean the pipeline and
prepare for the next test.
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PART 02
Reagents
Reagents
2.1.1 System reagent(Auffer)
Application Flushing and cleaning the measuring cell
Storage Temp. 4°C – 30°C
Validity Period
2 Years
(before opened)
Validity Period
28 Days
(after opened)
DO NOT touch
without Gloves!
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Reagents
2.1.2 System reagent(Buffer)
Provide TPA, provide reaction material
Application
for luminescence reaction
Validity Period
2 Years
(before opened)
Validity Period
28 Days
(after opened)
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Reagents
2.1.3 System reagent(System water)
System Initialization, Cleaning S/R and
Application
Aspiration Probe
Validity Period
2 Years
(before opened)
Validity Period
28 Days
(after opened)
System Water (9L distilled water : 1L Concentrated Washing

Buffer)
• Must put water into System Water Bucket before buffer, then
stand for 2 hours before use to eliminate bubbles.
Concentrated washing buffer • 1L / bottle, 6 bottles / carton.
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Reagents
2.2 Immunoreagent
Contains 1 bottle of immunoreagent kit,
QC card, two bottle of calibrator, two Use for Sample Testing, Calibration and
bottle of QC. Application
QC for each item.

Note: The consumption of each item of
immune reagents varies, please refer to Validity Period
Refer to IFU
the reagent’s IFU. (before opened)
Validity Period Refer to IFU
Immunoreagents cannot be stored (after opened)
upside down to prevent reagent
leakage.
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Reagents
2.2 Immunoreagent
Dilution Water Biotinylated Ruthenium- Magnetic
(Some items may not Ab/Ag labeled Ab Bead
include it )
One Immnuoreagent Kit

One QC card: • For item such as 25-OH VD,
• contain the QC Information. it may contain two
Immunoreagent kits.
Two QCs ：
Two Calibrators • Items for Cardiac Marker use
• Some items such as Multi Control, QC is not
Gastrin-17 contain three included in the
calibrators. Immunoreagent.
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Reagents
2.3 Measuring Cell Maintenance Reagent
Measuring Cell Maintenance (Every 2

Application
Weeks or After 2500T)
Validity Period
2 Year
(before opened)
Validity Period
28 Days
(after opened)
Usage Volume 5ml each time (200ml Total)
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Reagents
2.4.1 Commissioning reagent – High Voltage Adjustment Reagent
High Pressure Adjustment: calibrate

analyzer to maintain consistency between
Application
analyzer (For Installation and Trouble
Shooting)
Validity Period
1 Year
(before opened)
Validity Period
28 Days
(after opened)
High voltage adjustment reagent
(In eCL8000 installation package)
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Reagents
2.4.1 Commissioning reagent – High Voltage Adjustment Buffer
High Pressure Adjustment: calibrate

analyzer to maintain consistency between
Application
analyzer (For Installation and Trouble
Shooting)
Validity Period
1 Year
(before opened)
Validity Period
28 Days
(after opened)
High Voltage Adjustment Buffer
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PART 03
Installation Requirement
Condition
Installation Requirement Condition
1.1 Space requirement
Ensure the required space for repair and maintenance. Considering the radiating of the instrument and the
liquid pipe behind the analyzer shall not squeezed, the installation of the analyzer should meet the
following requirements:
·The distance from the wall to left and right side doors of the analyzer should more than 50cm;
·The distance from the wall to back door of the analyzer should more than 50cm;
·Make sure there is enough space on the work surface for the analyzer and reagent bucket.
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1.2 Power requirements
·The analyzer must be used in a well-grounded condition. Before turning on the analyzer, make sure that
the input voltage meets the requirements of the instrument. Use UPS if necessary.
·The use of patch panels may introduce additional electrical interference and cause erroneous analysis
results. Please choose a place near the power outlet
Analyzer to avoid the use of patch panels.
• Voltage: A.C.100 ～ 240V
• Input power: 150VA
• Frequency: 50 / 60Hz
Earth
• Grounding
• Earth and Neutral wire: 0V< Voltage < 5V
• Between Live and Neutral wire: 100V ~ 240V
• Between Live ad Earth wire: 100V ~ 240V
Neutral
Live
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1.3 Environmental requirements
(1) Working temperature: 10 ℃ ～ 30 ℃

(2) Working humidity: ≦ 80%
(3) Atmospheric pressure: 75 kPa ～ 106 kPa
(4) The environment should be as clean as possible without dust, mechanical vibration, large noise sources
and power supply interference.
(5) It is recommended to evaluate the electromagnetic environment of the laboratory before operating the
equipment.
(6) Do not place the device near sources of strong electromagnetic interference, so as not to affect the
normal operation of the device.
(7) Keep away from brush-type motors, flashing fluorescent lights, and electrical contact devices that are
frequently switched.
(8) Choose a well-ventilated location to avoid direct pipeline or direct heat sources and fans.
(9) Avoid direct sunlight or place in front of heat and wind sources.
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PART 04
Preparation for Installation
 Preparation tools Accessory pack Unit
Concentrated washing buffer 1 bottle
1. Common repair kit Buffer 1 bottle
2. 3# & 6# internal hexagon Auffer 1 bottle

Incubation cup 5-6 league
3. 100-1000UL pippttor
10L bucket(With machine box) 2 PCS
4. Diluted water or deionized water, 9L+ High voltage adjustment
1 bottle
buffer
5. Voltmeter
 Before installation, be sure to check whether the main box, accessory
package and cold chain package are in place.
Cold chain package Unit
Immune reagent Each 1 box （ 100T ）
Ecl8000 Installation package 1 box
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1. Common repair kit 2. 3# & 6# internal hexagon
3. 100-1000UL pippttor 4. Voltmeter
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PART 05
Product Installation
1. 3D-arm assembly
2. Inmunoreagent compartment
3. Scanner region
4. Sample compartment
5 Printer
6. Display
7. System reagent compartment
8. Reaction plate
9. 2D-are assembly
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1. System water liquid port
2. System water liquid sensor port
3. Waste liquid port
4. Water liquid sensor port
5.USB port
6.Internet port
7. Serial port
8. Power outlet
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5.1 Removal of fixtures
During transportation, the 2D arm and 3D arms need to be fixed to prevent collisions ；
Use a No. 3 Allen wrench to remove the fixing plate, be careful not to drop the screws into the
instrument. The fixing method is as follows:
1. Four hex screws to fix the Y axis of the 3D arm
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2. The Z axis of S/R probe is fixed with two hex screws;
3. The Z axis of aspiration probe is fixed with two hex screws;
4. The X axis of two arm is fixed with two hex screws
2D-Arm Z axis 3D-Arm Z axis Two arm X axis
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5. After removing all, there are 4 metal parts and 10 hex screws
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5.2 Install the reference electrode
1. Unplug the instrument power cable
2. Remove the screws holding the right door of the instrument and remove the right door
3. Remove the five screws on the cover of the detection unit and remove the cover.
(Before removing the cover, make sure that the whole machine is turned off. The photomultiplier in
the measuring cell is sensitive to light and if exposed to natural light when turned on, it will burn out
the photomultiplier.)
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4. Remove the plug by turning it counterclockwise
5. Take out the reference electrode from the accessory kit
Reference electrode installation instructions:

 Check whether the reference electrode O-ring is on
the reference electrode and there is no misalignment
in the installation position. Poor sealing of the O-ring
will cause water leakage;
 Soak the reference electrode porous ceramic (near the
O-ring) in distilled water for 5 minutes with the gold-
plated plug side of the circuit facing up and keep it
dry;
 Install the reference electrode on the measuring cell,
and install the measuring cell on the instrument for
use (note that the reference electrode circuit
connector is plugged in, the measuring cell cable will
not hang on the rotating magnetic pendulum)
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6. Plug in the reference electrode cable
7. Close the detection unit cover and fix it
8. Install and secure the right door of the instrument 1.Confirm that the white cable is
tightly inserted and will not fall off
naturally
2.Since the thread is plastic, it should
be lightly twisted when screwing, and
do not damage the thread due to
misalignment
Make sure NO cables are hanging on this

Pendulum.
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5.3 System water and accessary connection
1. Install system water (bottom) and waste liquid (bottom) according to the mark
2. Connect power cable and scanner
Wireless
mouse,
keyboard
System water
port System water
sensor
Scanner
Waste liquid Waste liquid

port sensor
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5.3 System water and accessary connection
The system water connector must be inserted into the bottom and locked firmly,Press the bottom and the
spring will bounce.
Note:
System water
System water Poor system water
port
sensor connection will
cause initialization
failure
Waste liquid
Waste liquid
port
sensor
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5.4 Loading system reagents
1. Load system reagents Auffer and Buffer, remove the reagent cap and bubbles inside, then cover the silicone cap
2. Buffer on the left, Auffer on the right, do not make mistakes when closing the lid and cause cross
contamination.
3. After the analyzer turned on, Auffer and Buffer should wait for at least 30 min. to reach the desire temperature
(28±2℃) before use.
Silicon Cap Cover
(1) 2D arm (2) Cleaning sink

(3) Buffer (4) Auffer
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5.5 Check before starting
1.Check that the system water bucket is filled with sufficient system water.
2.Check that the waste liquid bucket is empty.
3.Check that reagent, S/R probe and aspiration probe look good and are free of dirt
and bends.
4.Check that the system water and waste liquid pipes are not squeezed or bent.
5.Check that Auffer and Buffer are opened.
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5.6 Switch on
1. Turn on the "Refrigeration" switch of the
instrument (located on the lower left side of
the instrument), and turn on the power of the
refrigeration system of the immune reagent Host
compartment.
2. Turn on the "host" switch of the
instrument (located on the lower left side of
the instrument), and turn on the power of the
host.
Refrigerator
No sequence of switches.
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5.6 Switch on
The “Host” switch can be turned OFF, when

“Refrigerator” switch is ON, so the
Immunoreagent can be kept in the
Immunoreagent Compartment after used.
BUT
When turning ON the “Host” switch, the

“Refrigerator” switch MUST be turned ON at
the same time, otherwise the “E-Stop” alarm
will be shown.
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5.7 Login
1.After turning on the instrument switch,
the control software will automatically
start running and perform system
initialization and self-test.
2.Enter the user name (admin) and
password (admin) in the [Login] dialog
box, and click [Login].
Init
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5.7 Login
Two Authority Levels for Accounts:
1. Admin: Result Checking, User Creation, Default account (username: admin, password: admin)
2. User: Default account (username: lifo, password:123)
New Admin and User can be created in the following step:
Init
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5.8 System Status & Warning
Status & Brief Description

Warning
Initializing System initializing and self testing.
Standby Standby for operation.
Warn Potential erroneous for testing of current
sample.
P-Stop Stop additional sample adding during sample
testing.
S-Stop Stop the testing for current sample.
E-Stop Stop all testing operation.
Testing During testing.
Finish Test Finished.
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5.9 Loading immunoreagent information
1. Before loading the inmunoreagent, you need to place the reagent to close the reagent card in the scanner
area to read the reagent information, and then put it into the immunoreagent compartment after the reagent
information interface pops up. (Left corner Bottom is the credit card area)
2. It is only allowed when the system status is "Standby" for reagent loading.
3. After loading, the reagent will need 30 mixing time for magnetic beads, before it can be use for testing.
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5.10 Loading and replacing the incubation cup
1.Each 25 incubation cup are a group, and 100 incubation cup can be placed at the same time. If the remaining
cell volume on the [System] screen is insufficient or not loaded before starting the test, follow the steps below
to load or replace the cuvette.
1.1 Open the front cover of the instrument, you can see the incubation cup loading area; The cover plate of the
incubation cup must be
1.2 Select the area to be replaced or loaded on the interface; covered in the designated
1.3 The corresponding area of the incubation cup will be transferred to the loading area; position and cannot be
1.4 Take out the used incubation cups, if not, skip this step; shaken left and right.
Otherwise, a probe will
1.5 Place the new incubation cups in the loading area and click [Save]. occur during the incubation
1.6 If you need to replace or load cups in other areas, please repeat steps 2, 3, 4, 5 of the sample
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5.11 Blank test
Enter “Advanced” →
“Performance Maintenance” →
click “Blank Test” → click “Start”.
Account for “Advanced”:

Username: root
Password: lifo5689
After the test, check the 10 Base

Line values.
The Range for Base Line value
should be 30,000 – 600,000, and
the CV should be ＜ 1%.
If the CV is >1%, repeat the Blank
Test until the Base Line values are
stabilized.
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5.11 Blank test
The main purpose of Blank Test
is to measure and check the
signal value of Buffer reagent.
It should be done when

changing Buffer with new lot
number.
Check the 10 signal values

displayed in the Buffer column,
delete the first value, and check
the average result and CV. When
the CV is ＜ 5%, and the
average result is in the 200 -
1000. The blank test is
completed.
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5.12 High pressure adjustment After high pressure adjustment, the cuvette assigned to A in the tray needs to be replaced
1.The high-pressure adjustment reagent is with a new one, because the data of the cuvette used for high pressure adjustment will not
be recorded.
placed in the immune reagent compartment
No. 3 reagent position, the Buffer reagent is
replaced with the high-pressure adjustment
BUFFER reagent, When changing buffer
reagent, the instrument will display whether to
replace the reagents. If yes, the instrument will
count down 30 minutes to restore the
temperature to the reagents. If the reagent is
stored at room temperature(Around 25℃ to
30℃ ） , it can be used directly."→"
2. Click "High pressure Adjustment " to start

the test. When the tested 10 signal values
(without the first value) are within the range of
100,000 ± 5000 and the CV value is ＜ 3%,
the high pressure adjustment is completed.
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5.12 High pressure adjustment
3. If the high-pressure adjustment test signal is not in the range of 100,000 ± 5000, and the retest result is still
abnormal, first confirm whether the reagent is contaminated. If the signal value is still abnormal, please adjust
the potentiometer. Here is how to adjust the potentiometer:
(1). Unscrew the two screws fixing the upper cover of (2). Open the upper cover of the instrument and
the instrument(On the back side). disconnect the connectors of the two fans.
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(3).Stand on the right side of the instrument to adjust the potentiometer. Viewed from the right side, the potentiometer is located in the lower
right corner of the top.
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(4). The jumper corresponds to the potentiometer one (5). Now the jumper caps are connecting the jumpers in
by one. Connect a set of Jumpers with two jumper caps the middle, so the potentiometer in the middle can be
to activate the corresponding potentiometer(Only one adjusted.
set of jumpers can be connected at a time). Use a screwdriver to turn the screw on the
potentiometer clockwise for one round(360°),the value
of high voltage adjustment will be reduced by 6000.
One turn counterclockwise will increase the high
voltage adjustment value of 6000.
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If jumpers on the left side are connected, the Adjusting the potentiometer on the right, the change of
potentiometer on the left can be adjusted; The jumper high voltage value per revolution is more than 6000.
on the right corresponds to the potentiometer on the
right.
Clockwise rotation decreases the high voltage value
and counterclockwise rotation increases the high
voltage value.
However, when adjusting the potentiometer on the left,
the change of high voltage value per revolution is less
than 6000.
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4. After high pressure adjustment, the Incubation Cup in section A needs to be replaced with a new one,
because the usage data of the cups used for high pressure adjustment will not be recorded.
5. The Incubation Cups in section A can only be used for 2 rounds of High Pressure Adjustment Test, beware
to change the cups after 2 rounds of High Pressure Adjustment Test.
6. The High Voltage Adjustment Reagent can be only used for 5 rounds of High Pressure Adjustment Test, so
if the test results still do not meet the requirement after 3 rounds of High Pressure Adjustment Test, please
contact the Lifotronic engineer for further diagnosis.
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5.13 Calibration (PCT)
1. Take out the low value calibrator, high value calibrator, and immune reagent from the PCT kit.
2. Scan PCT reagent RFID card on RFID scanner
3. The loading reagent window will pop up, and then load the reagent kit to any position of the dedicated
reagent unit, such as the 10th position, there will be a “beep” when the reagent is loaded correctly.
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4. After loading, it will display the "Reagents" window. The magnetic beads will mix for 30 minutes.
(Because the mixing time can be long, we can load the reagents first, and perform the blank test and high pressure
adjustment during the mixing process)
5. On the sample rack, place the PCT high value and PCT low value calibrators (high value in front, low value in the
back), and be sure to take off the bottle cap; as shown, the sample rack is 1- 10 from left to right.
6. Push the sample rack with the calibrators into the sample compartment, and there will be a clicking sound when the rack
is in place;
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7. On the "Reagents" interface, click “Edit Calibration", and click “Pick" to select the corresponding low
calibration and high calibration positions, and confirm that the remaining number of incubation cups is
sufficient to complete the calibration test.
8. Click "Save". Confirm the magnetic beads has been stirred for 30 minutes, click "Start" test in the upper
right corner;
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9. After the test is complete, click “Start Cal." on the "Reagents" interface to check the calibration result;
Recommended Calibration pass conditions:
 High and low value signal deviation

requirements: ± 70%;
 High and low value deviation

differences (high value minus the low
value signal deviation) requires ± 30%;
 Test signal CV requirements: low value

<5%, high value <3%
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10. click “Cal." to complete the calibration;
11. If the calibration test does not meet the Calibration pass conditions, a warning will be shown above the
Calibration Curve graph indicate the test failed, then the calibration test may be repeated.
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5.14 QC (PCT)
1. After the calibration is
completed, scan the QC card in
the RFID, the following window
will pop up automatically, and
click "Loading".
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5.14 QC (PCT)
2.Place the low level and high
level QC on the sample rack (there
is no order requirement). Be sure
to unscrew the bottle cap.
3. Push the sample rack with the
QC to the bottom of the sample
compartment.
4. After loading, in the "Edit"
interface, first click on the
corresponding sample area, and
then click “Test Edit"
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5.14 QC (PCT)
5. Click the place where the low-
QC located, select “QC”, select
the corresponding lot number for 1 2
the low QC, and select L (low
level) for the QC level, do the
same operation of high-QC;
6. Select "OK" after you have
finished. 3
7. Click "Start" to start the test
4
5
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5.14 QC (PCT)
After the test is completed,
the QC results will appear in
the QC interface
In this area, you can choose

different items, high and
low values, test time, lots
number and other
information
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5.14 QC (PCT)
If the QC result dot on the

Graph is showing
YELLOW, the QC test
should be repeated.
If the second QC result dot
on the Graph is showing
YELLOW again, QC If the QC result dot on the
Failed. Graph is showing RED, QC
Failed.
If the QC result dot on the

Graph is showing GREEN,
QC Passed.
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5.15.1 Sample Test Process
1. Place the sample on the
sample rack, S / R probe has
no puncture function, so
remove the sample cap;
2. Push the sample rack with
the sample to the bottom of
the sample compartment; at
the same time, click "Patient
Information" to edit;
3. In the “Edit" interface, first
click the corresponding
sample rack, and then click
“Test Edit";
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4. Click the corresponding
location of the sample, select
"Sample" , then select the
sample type and number of
tests corresponding to the
sample, select PCT for the item,
and click "OK"
5. Click "Start" to start the test
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6. After the test is completed,
click the "Results" interface as
shown below to view the
sample test results.
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5.15.2 Sample Tubes
The sample rack contains 10 sample positions, each carrying a position identification barcode. The
minimum sample volume varies with respect of the specification of sample tube. The minimum sample
volume must be guaranteed for each sample tube, otherwise sample aspiration error may be caused. If
the sample volume is less than the dead volume, please transfer the sample to a smaller sample tube prior
to test. The sample vessels with the following specification and their corresponding dead are listed in the
table below:
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PART 06
Product Maintenance
Product Maintenance
6.1.1 Regular Maintenance – Daily Maintenance
Cleaning S/R Probe & Aspiration Probe
Purpose: Dirt on the S/R probe and Aspiration probe can cause contamination and carry over, and
affect test results. To prevent contamination, this part should be cleaned daily.
Precautions:
• All instrument power switches must be turned off;
• Wear suitable protective gloves;
• Take care not to damage the probe;
Materials Require:
• Clean gauze;
• Distilled or deionized water;
• 70% Alcohol;
Operating procedure:
1. Power off the instrument;
2. Move the probe to an easy-operate area;
3. Wipe the outer surface of the probe with alcohol-soaked gauze, and then wipe with distilled
water-soaked gauze;
4. Connect the instrument to the power supply and use it normally. 70% Alcohol Clean gauze
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Product Maintenance
6.1.2 Regular Maintenance – Weekly Maintenance
Cleaning Incubation Plate
Purpose: Leaking liquid on the incubation plate may cause the cuvette to stick, and this part needs to be cleaned
regularly.
Precautions:
• All instrument power switches must be turned off;
Materials Require:
• Clean gauze;
• 70% Alcohol;
• Cotton swab.
2. Remove the protective cover of the incubation plate;
3. Use a cotton swab with distilled or deionized water to wipe the holes of the incubation cup one by one;
4. Wipe the surface of the reaction plate with alcohol-soaked gauze, and then wipe again with gauze soaked in
distilled or deionized water;
Cotton Swab
5. Return the incubation plate cover to its original position;
6. Connect the instrument to the power supply and use it normally.
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Product Maintenance
6.1.2 Regular Maintenance – Weekly Maintenance
Cleaning Cleaning Pools
Purpose: The instrument has cleaning pools for flushing the S/R probe and aspiration probe. Dirt on the wash basin may cause
residues. To prevent contamination, this part should be cleaned at least weekly.
Precautions:
• Do not wipe with alcohol.
Materials Require:
• Clean gauze;
• Cotton swab.
2. Remove the probe arm;
3. Wipe the cleaning tank with a cotton swab moistened with distilled or deionized water;
4. Connect the instrument to the power supply and use it normally.
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Product Maintenance
6.1.3 Regular Maintenance – Fortnightly Maintenance
Measuring Cell Maintenance
Purpose: Contamination of the measuring cell may reduce the accuracy and precision of sample detection, and may even block the
measuring cell. In order to keep the measurement cell clean and maintain the performance of the measurement cell, the
measurement cell maintenance should be performed at least every two weeks or a total of 2500-3000 tests.
Precautions:
Materials Require:
• Measuring Cell Maintenance Reagent;
• Auffer.
1. Remove the Auffer from the system reagent compartment and put it into the measuring cell maintenance solution;
2. Click "Maintenance"-"Maintenance instruction" - “Measuring Cell Maintenance" in order. Perform the measurement pool
and wait for the maintenance program to complete. The whole process takes about half an hour.
3. Replace the measuring cell maintenance fluid with the normal Auffer.
4. Start 10 empty tests.
5. Complete the entire measuring cell maintenance
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Product Maintenance
6.1.3 Regular Maintenance – Fortnightly Maintenance
Measuring Cell Maintenance
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Product Maintenance
6.2.1 Irregular Maintenance – Quarterly Maintenance
Replace Pinch Valve Hose
Purpose: Using an old pinch valve hose can cause liquid to leak. Leaks will affect the accuracy of the pipetting volume and should
be replaced at least every three months.
Precautions:
• This maintenance must be performed by professional maintenance personnel.
Materials Require:
• Two new 180mm long silicon tubes;
• Screwdrivers
• Prepare two new 180mm long silicone tubes;
• Unplug the old silicone tube on the brown pagoda connector of the measurement adapter block;
• Use a new silicone tube to connect the upper and lower pagoda joints respectively;
• Clip the silicone tube to the inside of the corresponding pinch valve;
• Complete silicone tube replacement.
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Product Maintenance
Replacing Pinch Valve Hose
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Product Maintenance
After the pinch valve hose is changed, go to “Application” interface, “Advanced” → “HD Maintain” , click on “Replacement” for
“Pinch tube”.
Reset Counts for Pinch Valve Hose
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Product Maintenance
6.2.1 Irregular Maintenance – Annually Maintenance
Replace Measuring Cell
Purpose: In order to maintain the performance of the measuring cell and ensure the accuracy of the test result, it is recommended to
change the measuring every year or after 200,000T.
Precautions:
• All instrument power switches must be turned off and power cable must unplug;
• This maintenance must be performed by professional maintenance personnel.
Materials Require:
• A new measuring cell
• Screwdrivers NO POWER
CONNECTION
Measuring Cell Screwdrivers
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Product Maintenance
• Before replacing the measuring cell, make sure that the whole machine is powered off and no strong light is shining on the
measuring unit;
• Use screwdriver to loosen the combination screw of the upper cover of the measuring unit and remove the upper cover of the
measuring unit;
• Use screwdriver and a wrench to loosen the combination screws on the outside of the pull plate and the limit Allen screws on
the slider;
• Remove the inlet and outlet pipes connected to the measuring cell by counterclockwise;
• Unplug the measuring cell and photomultiplier tube from the PCBA;
• Take out the slide block and loosen the combination screw that is attached to the measuring cell (During operation, do not touch
the mirror surface of the photomultiplier tube, prevent strong light from shining on the mirror surface of the photomultiplier
tube, and do not directly touch the glass surface of the measurement cell Replace the prepared measuring cell on the slide block
assembly);
• Install the slide block assembly into the measurement unit;
• Insert the corresponding connectors on the measuring cell and the photomultiplier tube to the PCBA of the amplification board;
• Connect the inlet pipe and the outlet pipe to the measuring cell respectively;
• Use a wrench to lock the limit screws on the slide block, and use a screwdriver to lock the combination screws on the
attachment plate to fix the slide block assembly;
• Use a screwdriver to lock the upper cover of the measuring unit to complete the replacement of the measuring cell.
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Product Maintenance
Replacing Measuring Cell
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Product Maintenance
After the measuring cell is changed, go to “Application” interface, “Advanced” → “HD Maintain” , click on “Replacement” for
“Measuring cell”.
Reset Counts for Measuring Cell
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Product Maintenance
6.2.3 On-Demand Maintenance
Cleaning System Water Bucket
Contaminated system water bucket can affect analyzer performance. Clean the system water bucket as needed.
Waste Liquid Bucket or Direct Drainage Pipe

The waste liquid bucket must be inspected and emptied as needed. Filling the waste container can cause an alarm and
interrupt analyzer operation. This section applies to internal waste containers and does not need to be considered if direct
drains are used.
Cleaning system reagent compartment

If necessary, clean the system reagent compartment to remove leaking or residual liquid from Buffer and Auffer.
Protection of the measuring cell during long shutdowns

If the instrument is not used for more than 7 days, it is very important to properly perform the shutdown maintenance
procedure. Failure to follow these recommendations may damage the measuring cell. Depending on the shutdown period, a
number of different shutdown steps are recommended
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Appendix: Liquid line system
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THANKS
SHENZHEN LIFOTRONIC TECHNOLOGY CO., LTD.
4th Floor, Building 15, 1008 Songbai Road, Nanshan District, Shenzhen 518055, P.R.China
Tel: 0755-29060026 Fax: 0755-29060036 Web: en..lifotronic.com

eCL8000 Installation Guide

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eCL8000 Installation Guide

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eCL8000

Tripyridine Ru(bpy)3+ Ru(bpy)2+

Application Flushing and cleaning the measuring cell

Storage Temp. 4°C – 30°C

Storage Temp. 4°C – 30°C

Storage Temp. 4°C – 30°C

System Water (9L distilled water : 1L Concentrated Washing

Storage Temp. 2°C – 8°C

One Immnuoreagent Kit

Measuring Cell Maintenance (Every 2

Storage Temp. 2°C – 8°C

High Pressure Adjustment: calibrate

Storage Temp. 2°C – 8°C

High Pressure Adjustment: calibrate

Storage Temp. 4°C – 30°C

(1) Working temperature: 10 ℃ ～ 30 ℃

1. Common repair kit Buffer 1 bottle

2. 3# & 6# internal hexagon Auffer 1 bottle

Immune reagent Each 1 box （ 100T ）

Ecl8000 Installation package 1 box

3. 100-1000UL pippttor 4. Voltmeter

7. System reagent compartment

2. System water liquid sensor port

3. Waste liquid port

4. Water liquid sensor port

2D-Arm Z axis 3D-Arm Z axis Two arm X axis

Reference electrode installation instructions:

Make sure NO cables are hanging on this

Waste liquid Waste liquid

Silicon Cap Cover

(1) 2D arm (2) Cleaning sink

2.Check that the waste liquid bucket is empty.

5.Check that Auffer and Buffer are opened.

The “Host” switch can be turned OFF, when

When turning ON the “Host” switch, the

New Admin and User can be created in the following step:

Status & Brief Description

Account for “Advanced”:

After the test, check the 10 Base

It should be done when

Check the 10 signal values

2. Click "High pressure Adjustment " to start

Recommended Calibration pass conditions:

 High and low value signal deviation

 High and low value deviation

 Test signal CV requirements: low value

In this area, you can choose

If the QC result dot on the

If the QC result dot on the

Measuring Cell Maintenance

Replacing Pinch Valve Hose

Reset Counts for Pinch Valve Hose

Measuring Cell Screwdrivers

Replacing Measuring Cell

Reset Counts for Measuring Cell

Waste Liquid Bucket or Direct Drainage Pipe

Cleaning system reagent compartment

Protection of the measuring cell during long shutdowns

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