Oregano (Origanum

Vulgare) leaves extract as

antibacterial against
Streptococcus
pneumoniae
Chapter 1
 Contents
A. Overview of the study C.Statement of the
Problem

B.Research Paradigm D. Hypothesis

 Introduction
Overview of the study
Oregano is used medicinally for thousands of years, the leaves were applied topically to treat conditions
like skin sores, ease muscle pain, and act as an antiseptic.

Traditional treatments have utilized oregano to treat conditions like asthma, cramps, diarrhea, and
indigestion.Oregano is a rich source of bioactive compounds that have been pharmacologically tested for
treating a variety of infectious disorders and exhibit hepatoprotective, carminative, spasmolytic,
antibacterial, antiviral, and anticancer activities. Streptococcus pneumoniae are lancet-shaped, gram-
positive, facultative anaerobic bacteria with more than 100 known serotypes. Most S. pneumoniae
serotypes can cause disease, but only a minority of serotypes produce the majority of pneumococcal
infections.
B. Research Paradigm
Independent variables Dependent variable
Azithromycin (control)

100% Oregano leaves Mortality according to its zone

extract of inhibition

75% Oregano leaves extract

25% ethanol
C. Statement of the Problem
1) What is the antibacterial property of Oregano leaves
extract according to its zone of inhibition?

a. Azithromycin (control)

b. 100% Oregano Leaves Extract

c. 75% Oregano Leaves Extract and 25% Ethanol

2) Is there a significant difference in the antibacterial activity
of Oregano leaves extract according to its zone of inhibition?

a. Azithromycin (control)

b. 100% Oregano Leaves Extract

c. 75% Oregano Leaves Extract and 25% Ethanol

 Null Hypothesis

There is no significant difference in the antibacterial effect of

the Oregano (Origanum Vulgare) leaves extract on
Streptococcus Pneumoniae in alcohol solution.
Chapter 3
 Contents
A. Research Design B. Outline of General
Procedure

C. Statistical Data
Analysis Procedure
A. Research Design
This study entitled "Oregano (Origanum vulgare) Leaves Extract
antibacterial against Streptococcus Pneumoniae is an experimental
research that will make series of is an observation and testing to measure
the effectiveness of the product.This product is created to find an
alternative solution to the expensive and colorants with extreme amount of
chemicals.

The chosen research design is pretest-post test experimental group

design.
B. Outline of General
•Procedure
1.Plant Extraction
•2.Extraction Method

•3.Laboratory Testing
C. Statistical Data
Analysis Procedure
• Mean
• Mode
• Range
Thank you!