DNA

FINGERPRINTING
Presented by:
Haidar Sitie Rafidah (2006615010)

Supervised by:
Prof. Usman Sumo Friend Tambunan

BIOTECHNOLOGY CLASS OF 2021

PRESENTATION OUTLINE
1. The Structure of DNA

2. What is DNA Fingerprinting?

3. History of DNA Fingerprinting

4. DNA Electrophoresis Experiment

5. The Procedure of DNA Fingerprinting

6. DNA Fingerprinting Application

 01
The
Structure of
DNA
 THE STRUCTURE OF DNA

1
Roy, Tamal, Kai Szuttor, Jens Smiatek, Christian Holm, & Steffen Hardt. (2019). Conformation and Dynamics of Long-Chain End-Tethered Polymers in Microchannels.  Polymers 11(3): 488.
02
What is DNA
fingerprinting?
 What is DNA
Fingerprinting
?
DNA fingerprinting is a technique originally
derived from the use in medical genetics of
polymorphisms, silent variations between
the genomes of different persons2.

2
Housman, D. E. (1995). DNA on Trial — The Molecular Basis of DNA Fingerprinting. New England Journal of Medicine, 332(8), 534–535.
03
History of DNA
fingerprinting
 HISTORY OF DNA
FINGERPRINTING
• DNA fingerprinting was developed in 1984
by Alec. J. Jeffrey at the University of
Leicester while he was studying the gene
for myoglobin4.

• In 1987, British Baker Colin Pitchfork was

the first criminal caught using DNA
fingerprinting4.

• In 1990, California established DNA

fingerprinting banks for sex offenders4.

4
Jordan D and Mills D. (2021) Past, Present, and Future of DNA Typing for Analyzing Human and Non-Human Forensic Samples. Front. Ecol. Evol. 9:646130
04
DNA
Electrophoresis
Experiment
 DNA ELECTROPHORESIS
EXPERIMENT
• Electrophoresis is a
technique that can be used
to separate the DNA and
RNA fragments from one
another according to their
sizes5.

5
Cai, Y. (2020). Analysis on Gel Electrophoresis in Biology. E3S Web of Conferences, 145, 01009.
 DNA ELECTROPHORESIS
EXPERIMENT

6
Ylmaz, M., Ozic, C., & Gok, lhami. (2012). Principles of Nucleic Acid Separation by Agarose Gel Electrophoresis. Gel Electrophoresis - Principles and Basics. University of Kafkas, Turkey
05
The Procedure
of DNA
Fingerprinting
 THE PROCEDURE OF DNA
FINGERPRINTING

1. DNA isolation7
2. Digestion with restriction endonucleases7
3. Electrophoresis to separate different sizes7
4. Hybridization with probe specific for each
VNTR7

7
Housman, D. E. (1995). DNA on Trial — The Molecular Basis of DNA Fingerprinting. New England Journal of Medicine, 332(8), 534–535.
06
DNA
Fingerprinting
Application
 DNA FINGERPRINTING
APPLICATION
Parentag
1 e
Testing8

Forensic
2 Science9

DNA ID
3 Card10

8
Sathyan S, A. M. (2015). Application of DNA Fingerprinting in an Alleged Case of Paternity. Biochemistry & Analytical Biochemistry, 04(02):1000165.
9
Moumita S., Arjun R., I. & Mitashree M. (2017). DNA fingerprinting applications in forensic investigation. International Journal of Current Research, 9(01):45542-45546.
10
Pilcher, H. (2003). Genetics for everyone. Nature, 1476-4687.
 PARENTAGE TESTING
• DNA fingerprinting is widely used in
parentage testing around the world by
comparing different DNA sequences8.

• The test will be conducted using

Polymerase Chain Reaction (PCR)
technology11.

• The results of parentage testing are known

in DNA fingerprinting divided into three
columns, there are Mother, Child, and
Father11.
8
Sathyan S, A. M. (2015). Application of DNA Fingerprinting in an Alleged Case of Paternity. Biochemistry & Analytical Biochemistry, 04(02):1000165.
11
Garibyan L, Avashia N. Polymerase chain reaction. J Invest Dermatol. 2013;133(3):1-4.
 FORENSIC SCIENCE
• The DNA of a criminal suspect can be
determined by using DNA fingerprinting
technology9.

• Scientists find the marker in a DNA sample

by designing probes of DNA that will each
seek out and bind to a complementary DNA
sequence in the sample9.

• Forensic scientists compare these DNA

profiles to determine the suspect with
evidence samples9.

9
Moumita S., Arjun R., I. & Mitashree M. (2017). DNA fingerprinting applications in forensic investigation. International Journal of Current Research, 9(01):45542-45546.
 DNA ID CARD
Personal
Information

• The DNA ID Card contains your

personal information (Photo, Name,
Gender, Date of Birth) and DNA
profile10.

DNA
Profile

10
Pilcher, H. (2003). Genetics for everyone. Nature, 1476-4687.
THAN
K
YOU