Rr-61 Button Mushroom

P. R.
Pote Patil Education & Welfare Trust’s

P. R. Pote Patil College of Agriculture
Kathora road, Amravati, Maharashtra
(Affiliated to Dr. Panjabrao Deshmukh Krushi Vidyapeeth, Akola)
_____________________________________________________________________________
MUSHROOM CULTIVATION TECHNOLOGY

ELM PATH -486
Subject:- Preparation of Commercial Spawn

& Spawning for Button Mushroom
Presented by:- Miss Sakshi Sudhir Ardak
Enroll no.- RR-61
Under Guidance of:- Section of Plant Pathology
P. R. Pote Patil Education & Welfare Trust’s
• Content :-
• Preparation of spawn
for commercial
production of button
mushroom
• Spawning & bag filling
for button mushroom
cultivation
• Preparation of spawn for commercial

production of button mushroom :-
1. Spawn :-
• Spawn is a pure mycelial growth, grown on sterilized

cereal grains, straw or swit.
• Spawn is the seed of mushroom.
• The spawn (seed of mushroom) is a pure culture of the
mycelia grown on a special medium.
• The medium is prepared by the grains of wheat, rye,
sorghum or bajra along with some ingredients.
Master spawn :-
• The spawn which is prepared by inoculating sterilized

cereals grain or straw or sawdust pure culture is called as
master spawn.
• Master spawn is nothing but the mushroom fungus
grown on a grain based medium
• Pure culture raised either from tissue or spores is
inoculated in a suitable substratum,which provides food
to the mycelium
Commercial spawn :-
• Spawn prepared by inoculating sterilized cereal grains,

straw or sawdust with master spawn.
• Master spawn or master culture bottles are further used for
inoculation of large number of other grain bags / bottles
prepared by the same technique and resultant is the
commercial spawn
• Equipment and Materials required:-

Equipments:-
Sr. Equipment required Sr. Equipment required
No No
1. Autoclave 6. pH meter
2. Isolation Chamber 7. Gas
3 Incubator 8. Stove
4. Weighing balance 9. Vessel with led
5. Refrigerator 10. Aluminium mesh

Materials:-
Sr. Material required Sr. Material required
No No
1. Milk or saline bottles 5. Polypropylene bag
2. Cotton wool 6. Rubber band
3. PVC pipe ring 7. Cereal grain
4. Gunny bags 8. Jute cloth
• Chemical required :- Gypsum, mercuric

chloride,CaCO3
• Steps for Preparation of Spawn for

commercial button mushroom production:-
1. Select unbroken, not very old & clean sorghum or wheat grains.
2. Boil the grains in water. Take precaution that the grains don’t
split to give out starch & they don’t become too soft.
3. Remove excess water using aluminium mesh & spread the

grains over jute cloth.
4. Then add 2% CaCO3 & 2% gypsum OR 3-4% CaCO3 alone to

the grain on wet weight basis. Mix it well & fill it in polythene
bags of suitable size.
5. Then plug this bags tightly & sterilize it @20lbs/psi for 2

consequent days
6. Now, inoculate the sterilised bottles with pure culture and

inoculate at 24+ - 2°C for 2 weeks
7. In this time the mycelial growth covers the grains &

conspicuous whitish thread like growth is observed on the grains.
8. Commercial spawn can be prepared by inoculating 20

polypropylene bags from 1 bottle of master spawn
1A 1B 1C
1A :- Boiling of Grain
B :- Mixing Gypsum & CaCO3 with grains
C :- Filling grain in polythene bags
1D 1E 1F
1G 1H
1D:- Grain Sterilization E:- Inoculate Sterilized bottle with pure culture F:- Master spawn
G:- Inoculate Master spawn for preparation of commercial spawn H:- Inoculate commercial spawn
• Spawning & Bag filling for Button

Mushroom Cultivation :-
Spawning
Tray System of cultivation Bed system of Cultivation
1) Single layer 2) Double layer 3) Through 4) Spot

spawning. spawning spawning. spawning.
• The process of mixing spawn with compost is called

Spawning.
A. Tray system of cultivation:- The compost when

ready is taken in trays leaving about 5 cm from the top.
• Single layer spawning:-

The grain spawn is scattered
uniformly all over the compost surface in a tray, which is
then covered with a thin layer of compost 200g/m bed
spawn.
• Double layer spawning:-

The trays are half filled &
spawn is scattered. Then the tray is filled & completely
compost & spawn is applied as above & covered with a
thin layer of spawn.
• Through spawning/mixed spawning:-

The required
quantity of spawn is mixed with the compost & filled in
trays or polybags.
• Spot spawning:-
The compost is filled in trays &
holes of 2.5-5cm deep are made with fingers about 8-
12cm apart in rows. About 5gm spawn is introduced in
to cavities & covered with a thin layer of compost.
After spawning, cover the beds with sterilized
newspaper & sprinkle water over the papers to maintain
moisture level of compost
The temperature should be around 25±2°C with relative
humidity of 85-90%.
B) Bag system of cultivation:-
1. In this method polythene covers of size 35“x 24" with

150 gauge thickness are used.
2. The spawn is mixed with the compost @
500-750gm/100kg compost.
3. Then mixed compost is filled in polythene bags @ 15-
20kg/bag.
4. Finally, the beds are covered with newspaper.
.

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P. R.

Pote Patil Education & Welfare Trust’s

MUSHROOM CULTIVATION TECHNOLOGY

Subject:- Preparation of Commercial Spawn

• Preparation of spawn for commercial

• Spawn is a pure mycelial growth, grown on sterilized

• The spawn which is prepared by inoculating sterilized

• Spawn prepared by inoculating sterilized cereal grains,

• Equipment and Materials required:-

2. Isolation Chamber 7. Gas

4. Weighing balance 9. Vessel with led

5. Refrigerator 10. Aluminium mesh

1. Milk or saline bottles 5. Polypropylene bag

2. Cotton wool 6. Rubber band

3. PVC pipe ring 7. Cereal grain

4. Gunny bags 8. Jute cloth

• Chemical required :- Gypsum, mercuric

• Steps for Preparation of Spawn for

3. Remove excess water using aluminium mesh & spread the

4. Then add 2% CaCO3 & 2% gypsum OR 3-4% CaCO3 alone to

5. Then plug this bags tightly & sterilize it @20lbs/psi for 2

6. Now, inoculate the sterilised bottles with pure culture and

7. In this time the mycelial growth covers the grains &

8. Commercial spawn can be prepared by inoculating 20

• Spawning & Bag filling for Button

Tray System of cultivation Bed system of Cultivation

1) Single layer 2) Double layer 3) Through 4) Spot

• The process of mixing spawn with compost is called

A. Tray system of cultivation:- The compost when

• Single layer spawning:-

• Double layer spawning:-

• Through spawning/mixed spawning:-

B) Bag system of cultivation:-

1. In this method polythene covers of size 35“x 24" with

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