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Qualitative Characteristics of Cell Culture
Qualitative Characteristics of Cell Culture
Culture
Introduction:
2. Proliferation:
The rate of cell division and proliferation is an
essential characteristic of cell culture. Cell growth can
be assessed by counting cells over time or measuring
DNA synthesis.
3. Secretion:
Many cells secrete specific substances such as hormones, growth
factors, enzymes, or extracellular matrix components. Secretion
can be detected through biochemical assays or immunostaining.
4. Motility:
Certain cell types, such as immune cells or fibroblasts, exhibit active
motility. The ability of cells to migrate or move can be assessed by
tracking individual cell .
5. Cell Viability:
The percentage of living cells within a culture is an important indicator of
cell health. Viability can be determined using various staining techniques or
through cell exclusion dyes.
6. Metabolism:
The metabolic activity of cells in culture can be evaluated by measuring
oxygen consumption, glucose uptake, lactate production, or other metabolic
indicators.
7. Differentiation:
Certain cell types have the ability to differentiate into specialized cell types.
8. Cell Aggregation:
Some cell types naturally form aggregates or clusters, while others remain
as single cells. The tendency of cells to aggregate can be assessed by
observing cell behavior in culture.
Conclusion: