Anti-inflammatory activity of drugs using

Carrageenan induced paw edema model

612 Pharmacology Lab

4th year
Dr. Adnan Iqbal
 Requirements
• Albino Rat (200-250gm)
• Digital Plethysmometer
• Carrageenan Solution 1% (o.1ml)
• Aspirin (300mg/kg)
Inflammation Vascular Events video
Principle
 Procedure
• 0.1 ml of 1% carrageenan solution in normal saline is injected to the sub plantar tissue of the
left hind paw of the rat.

• The paw volumes of the rat are measured using Digital Plethysmometer at intervals of
5min,10 and 15min.

• The anti-inflammatory agent is administered orally by using feeding tube.

• Wait for at least 15mins.

• Again measured the paw volumes of the rats at 30mins, 35 and 40 mins.
Observation and Results

Group no. Drug Dose Carrageenan solution Anti-inflammatory agent

5min 10min 15min 30min 35min 40min

Carrageenan 0.1ml 0.7 0.7 0.9 0.8 0.7 0.7

solution 1%
1            
           
Aspirin 300mg/kg
           
           
           

Anti-inflammatory effect of drug is observed.

Paw volume before administration of drug is : 0.9
Paw volume after administration is : 0.7