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Lecture 3-3
Lecture 3-3
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Respect your parents, respect your
teachers
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Mechanisms of Receptor Regulation
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Steroid and Thyroid Hormone Receptor Resistance
Syndromes
• Receptor inactivity→
• Hormone defficiency
• elevated levels of the circulating hormone ligand
• increased (or inappropriately detectable) levels of the relevant trophic
regulatory hormone (eg, ACTH, TSH, FSH, or LH)
• Androgen receptor mutation
• X-linked
• Thyroid receptor mutation
• β form of the receptor
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Receptors Upregulation
• Increase in the number of receptors for
the particular hormone
prevailing levels of the hormone have
been low for some time
• A hormone can also up-regulate the
receptors for another hormone
Thyroid hormone up-regulates cardiac
adrenergic receptors
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Control of Hormone Release
• Constitutive (basal) and stimulated (peak
levels).
• The periodic and pulsatile release of hormones
• Plasma levels of hormones Oscillate
throughout the day, showing hormone specific
peaks and troughs.
• Regulated by interaction and integration of
multiple control mechanisms
• hormonal, neural, nutritional, and
environmental factors
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Control of Hormone
Release
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Neural Control
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Hormonal Control
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Environmental Factors
• Lifestyle factors-showing the clearest association with TSH and thyroid
hormones were smoking, body mass index (BMI) and iodine.
• Smoking mainly led to a decrease in TSH levels and an increase in
triiodothyronine (T3) and thyroxine (T4) levels, while BMI levels were
positively correlated with TSH and free T3 levels.
• Excess iodine led to an increase in TSH levels and a decrease in thyroid
hormone levels.
• Among the pollutants analyzed, a decrease in thyroid hormone levels after
exposure to perchlorate was observed by most studies.
doi: 10.3390/ijms22126521
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Feedback control mechanism
• Release of one hormone can be influenced by more than one of these
mechanisms (neuronal, hormonal, Nutrient)
• The neuroendocrine system adapts to a changing environment to maintain
homeostasis
• The responsiveness of target cells to hormonal action leading to regulation of
hormone release constitutes a feedback control mechanism.
Negative feedback & Positive Feedback
• adaptive changes do not lead to pathologic conditions
• short- and long-term adaptations to changes in the environment
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Levels of feedback mechanisms
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Assessment of Endocrine Function
• Disorders of the endocrine system result from
alterations in hormone secretion or target cell
responsiveness to hormone action
• Decreased responsiveness
• a decreased number of hormone receptors,
• a decreased concentration of enzyme activated by the
hormone,
• an increased concentration of noncompetitive inhibitor,
• or a decreased number of target cells
• Hormone sensitivity
• Higher hormone concentrations to produce 50% of the
maximal response
• decreased hormone-receptor affinity, number
• increased rate of hormone degradation
• increased levels of antagonistic or competitive
hormones.
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Hormone Measurements RIA
https://www.youtube.com/watch?v=Cs1LvCyEGKg
Cross-reactivity Prohormone
Degradation
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Limitations of RIA
• Lack of Specificity
• cross-reactivity of the antibody with more than one hormone
• Hormone sharing homology can lead to nonspecific recognition by the
antibody
• Heterogeneous form of hormone in the plasma
• Prohormone, enzymatic degradation
• Presence of circulating endogenous antibodies
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Hormone Measurements Immunometric Assay
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Interpretation of Hormone Measurements
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Interpretation of Hormone Measurements
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