BIOMATERIALS LAB

Drug Delivery & Controlled Release

Monday, March 19th and Tuesday, March 20th

BME 245/445 – Lab 4

PRE-LAB
 Sign-in
 Quiz (Lin. 2009)

BME 245/445 – Lab 4

Big Picture: Drug Delivery

 Control drug delivery rate, dose & location

Toxic Level
Therapeutic Range With Delivery
Drug Concentration

Device
  Period of
Efficacy

Traditional
Delivery

Time

BME 245/445 – Lab 4

Big Picture: Drug Delivery
 Monolithic Device
 Drug is released by diffusion out of polymer matrix
 Release rate depends on:
 Drug loading
 Drug size
 Mesh size
 Shape of device
http://www.sigmaaldrich.com/materials-science/biomaterials/tutorial.html
 Applied in Norplant – implantable contraceptive device
 Release hormone over 5 years
 Discontinued – unpopular, not cost-effective

BME 245/445 – Lab 4

LINEAR MOLECULES

 PEGMA (2, 10, 20kD)

 Increase number of repeat units (n)
 Alter mesh size and thus rate of BSA release

Small Mesh Size Large Mesh Size

BME 245/445 – Lab 4

METHACRYLATE POLYMERIZATION
Step-growth
Polymerization

1 “Arm”

PEG MA

(Lin, C.C., Pharmaceutical Research. 26(3): 631-643.

2009)

BME 245/445 – Lab 4

SECTION 1
PEGMA Network Synthesis
1. 10wt% PEGDA in PBS+BSA (10 mg/mL)
2. 10% photo-initiator by volume (LAP)
3. 40μL placed in 1mL syringe (tip cut off)
 to create cylindrical hydrogel sample geometry
4. exposed to UV-lamp for 10min
5. Remove and place in PBS

PEG (mg) PBS solution (μl) Initiator (μl)

28.3 225 25
 SECTION 2
Protein Collection Time Course (done by TA)
 BSA in hydrogel will release into surrounding PBS
 PBS will be routinely changed (stored in -80°C freezer)
 BSA concentration will be assessed for total release

BME 245/445 – Lab 4

SECTION 3
Protein Detection Assay – Coomassie
 Compared unknowns to known [BSA] standards
 Measure Absorbance at 590nm

BME 245/445 – Lab 4

From plate reader….

Absorbance at 590 nm
  PEG MW
Sample 2 kDa 10 kDa 20 kDa
#

1      
2      
…      
n      

BME 245/445 – Lab 4

What we are interested in….
% BSA released

Time

BME 245/445 – Lab 4

 Theoretical Governing Eqns

Drug diffuses out

z=L

z=L/2

  z=0
Drug diffuses out

BME 245/445 – Lab 4

 Governing Eqns
Drug Concentration CA

time
0 C0

z=0 z=L

BME 245/445 – Lab 4

Simplifying Governing Eqns

BME 245/445 – Lab 4

Mesh Size Calculations

BME 245/445 – Lab 4

Safety
 General
 PEG/PBS/BSA are relatively benign
 Lab glasses, gloves, closed toed shoes
 No food, no drinks, no applying makeup
LAB TEAMS- Monday
TEAM A TEAM B TEAM C TEAM D TEAM E
2k 2k 10k 10k 20k
Ripa Chris Farrar Matt Hazelett Caressa Chen Ruth Davis
Chowdhury
Eric Comeau Christine Mclin Rachel Rakvica Tonima Quabili Corinne Lampe

Margaret Fettis Kristen Yi Rao Ka Lai Tsang Mitchell Long

Harding
Bingqing Wang Andrea Wong Stephen Zhang Chris Schmitt

BME 245/445 – Lab 4

LAB TEAMS- Tuesday
TEAM F TEAM G TEAM H Team I
2k 10k 20k 20k
Ibrahima Bah Raymond Seung Hoon Lee Alex Kotelsky
Goo
Joseph Sara Nowacki David Narrow Jennifer
Mozingo Williams
Carly Spencer Erin Yoon-Suk Yi
Sombric Putnam Schnellinger
Nicole Varble Shijie Zhong Sean Rodriguges Sijia Zhang

Douglas Clift Robert Shijie Zhong

Mcwhorter

BME 245/445 – Lab 4