ABSOLUTE EOSINOPHIL

COUNT

Prepared by: Reviewed by:

Mr. Mohideen Sharfan S A PPT Review committee
Assistant Professor
Medical Laboratory Technology

PREPARED BY MOHIDEEN SHARFAN S A

 LESSON PLAN
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 SPECIFIC LEARNING OBJECTIVE
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At the end of the session student should be able

to:
Describe different methods used to detect AEC.

Explain the principle and procedure of manual method

AEC.
To know the normal values and the abnormal clinical

significance.
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 INTRODUCTION
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An absolute eosinophil count is a blood test that

measures the number of one type of white blood cells

called eosinophil.
Eosinophils become active when you have certain

allergic diseases, infections, and other medical

conditions.

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Different Methods:

 Manual method by Improved Neubarchamber.

 P.Smear method

 Automated Method

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 Manual Method
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Equipment

• Improved Neubauer counting chamber.

• WBC pipette

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 DILUTING FLUID
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Dunger’s fluid

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 TECHNIQUE
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Gently mix the blood in the EDTA vial, so that the cells mix

well with plasma.

Draw the blood in the WBC pipette up to mark 1.

Wipe off the excess blood from sides of the tip of the

pipette.
Dip the tip of the pipette in the Dunger’s fluid and draw

the fluid up to mark 11. The dilution is 1 in 10.

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Holding the pipette horizontally in its long axis, rotate it

slowly to ensure thorough mixing of blood and diluent.

This is facilitated by the white bead in the bulb.
Place the cover slip on the cleaned ruled area of the

counting chamber.
Discard the first 2 to 3 drops (since the fluid has not

mixed with blood) of WBC fluid from the pipette.

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Charge the chamber by placing the tip of the pipette

just under the cover slip and fluid flows under it by

capillary action. Allow till the counting chamber is just
filled.
Wait for 5 minutes for the eosinophils to settle in the

chamber.

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Count the number of eosinophils in the 4 corner

squares under the microscope using a low power

objective.
Eosinophils are identified because of their bright red

granules and count should be done within 30 minutes.

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 P.Smear showing Eosinophil
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 NORMAL RANGE OF EOSINOPHIL COUNT
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Eosinophils constitute 1–6% of circulating WBCs.

The range of absolute eosinophil count is 40–450

cells/cu mm (0.04–0.45 × 109 /L).

Increased eosinophil count defined as an eosinophilia

more than 450/cu mm.

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 CLINICAL SIGNIFICANCE
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 Allergic/atopic conditions

• Asthma
• Urticaria

• Hay fever

• Drug reactions
• Allergic rhinitis

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 Parasitic infestations (with tissue invasion)

• Roundworm infestation
• Hookworm infestation
• Filariasis

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 Skin diseases

• Dermatitis (eczema)
• Pemphigus
• Scabies
• Dermatitis herpetiformis

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 Hematological diseases

• Chronic myeloid leukemia

• Polycythemia
• Hodgkin lymphoma
• Acute myelomonocytic leukemia
• Eosinophilic leukemia

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 Miscellaneous

• Tropical eosinophilia
• Pulmonary eosinophilia
• Loffler's syndrome
• Hypereosinophilic syndrome
• Eosinophilic granuloma

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 CALCULATION
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Absolute eosinophil count (AEC) = Total number of

eosinophils in 4 squares × 25.

In P.Smear

Total no of eosinophil × TLC / 100

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 SUMMARY
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An absolute eosinophil count is a blood test that

measures the number of one type of white blood cells

called eosinophils.
Eosinophils become active when you have certain allergic

diseases, infections, and other medical conditions. Blood

is comprised of red blood cells, platelets, and various
white blood cells.

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 FAQ
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Write the principle and procedure of manual method of

Absolute eosinophil count. (4)

Causes of increased AEC. (2)

Composition of Dungers fluid. (2)

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 REFERENCE
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Nayak R, Rai S. Essentials in Hematology and clinical

pathology.2nded.India:Jaypee the health science

publisher;2017.

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