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    4.4 - Animal Cell Culture

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    Animal cell culture involves removing cells from an animal and growing them under sterile and controlled conditions outside of the body. Some key events in the history of animal cell culture include Harrison successfully growing frog nerve cells in vitro in 1907, Lewis and Lewwis developing the first liquid media for cell culture in 1911, and the use of T-flasks as specifically designed cell culture vessels in 1923.

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    4.4 - Animal Cell Culture

    Uploaded by

    damsavi
    12 views13 pages
    Animal cell culture involves removing cells from an animal and growing them under sterile and controlled conditions outside of the body. Some key events in the history of animal cell culture include Harrison successfully growing frog nerve cells in vitro in 1907, Lewis and Lewwis developing the first liquid media for cell culture in 1911, and the use of T-flasks as specifically designed cell culture vessels in 1923.

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    Animal cell culture involves removing cells from an animal and growing them under sterile and controlled conditions outside of the body. Some key events in the history of animal cell culture include Harrison successfully growing frog nerve cells in vitro in 1907, Lewis and Lewwis developing the first liquid media for cell culture in 1911, and the use of T-flasks as specifically designed cell culture vessels in 1923.

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    12 views13 pages

    4.4 - Animal Cell Culture

    Uploaded by

    damsavi
    Animal cell culture involves removing cells from an animal and growing them under sterile and controlled conditions outside of the body. Some key events in the history of animal cell culture include Harrison successfully growing frog nerve cells in vitro in 1907, Lewis and Lewwis developing the first liquid media for cell culture in 1911, and the use of T-flasks as specifically designed cell culture vessels in 1923.

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    Animal cell culture

    Cell culture
    • Cell culture is the process by which prokaryotic,
    eukaryotic cells are grown under controlled
    conditions.
    • In 1885 for the first time maintained embryonic chick
    cells in a cell culture.
    • Cell culture was first successfully undertaken by Ross
    Harrison in 1907.
    • successfully grew animal tissue (frog neuroblasts) outside
    the body
    What is Animal cell culture..?
    • Cell culture refers to the process by which animal cells are grown
    in a controlled artificial environment.
    • In a cell culture technique, cells are removed from an animal and
    grown subsequently in a favorable environment under sterile
    conditions.
    History

    1885- Maintained embryonic chick cells in a saline culture by Roux


    1897-Survival of cells isolated from blood and connective tissue in serum and plasma
    by Loeb
    1907- Cultivated frog nerve cells in a lymph clot and
    observed the growth of nerve fibers in vitro for several weeks
    By Harrison
    1911- First liquid media consisted of sea water, serum, embryo
    extract, salts and peptones
    By Lewis and Lewwis
    1923- T-flask as the first specifically designed cell culture vessel
    Carrel and Baker
    1981 : Mouse Embryonic Stem Cell
    1985: Human growth hormone produced from recombinant bacteria
    1996 : The First mammal cloned from adult cells (Dolly, the sheep)
    • Graph showing the salient features of cell culture
    with evolution of a cell line

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