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Iodine Test
Iodine Test
IODINE TEST
TEST FOR
POLYSACCHARIDES
GROUP 6
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INTRODUCTION
The iodine test is a biochemical
test to detect and distinguish
certain polysaccharides (starch,
dextrin, and glycogen) from
monosaccharides and
disaccharides, and
polysaccharides such as cellulose .
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OBJECTIVES
REAGENTS MATERIALS 05
PROCEDURE
STEP 1
Take the 4 labeled test tubes in the test tube stand. Using
different droppers or pipettes, add 1 ml each of the test
samples in the tubes. Take 1 ml of distilled water in the
4th tube (Negative Control). Take the cellulose cotton
sample.
STEP 2
Add 3 drops of the dilute iodine solution to each of the
four tubes and to the cellulose sample. Observe the
change in color in the samples. 06
RESULTS
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TEST
COLOR
SAMPLE
DISTILLED WATER
(NEGATIVE YELLOW NEGATIVE
POSITIVE CONTROL)
TEST
CELLULOSE YELLOW NEGATIVE
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RESULTS
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DEXTRIN
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• 1/2 mL of fresh dextrin solution • Test Tubes
• Iodine solution • Droppers or Pipettes
REAGENTS MATERIALS 11
PROCEDURE
STEP 1
Using a dropper or pipette, add 1/2 mL of fresh dextrin
solution to the test tube.
STEP 2
Add one drop of iodine solution into the dextrin solution
then check for color change.
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DATA AND
NEGATIVE
TEST
RESULTS
When you add iodine to a solution, the color changes,
indicating that the solution contains polysaccharide.
The nature of the polysaccharide is determined by the
color produced.
If violet color appears, dextrin is present, and it
POSITIVE signifies a positive result. There is no dextrin present
TEST if the yellow color of the iodine reagent just becomes
diluted.
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QUESTIONS &
ANSWERS
QUESTION AND ANSWER
WHAT IS THE PRINCIPLE BEHIND THE IODINE TEST?
It is based on the principle that polyiodide ions, mainly the triiodide ions, present in
iodine solution form colored absorption complexes with the helical chains of glucose
residues of certain polysaccharides. The triiodide and pentaiodide ions formed are linear
and slip inside the helix structures, which then produces visual colors upon contact with
these polysaccharide molecules.
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POLYSACCHARIDE COLOR
AMYLOS BLUE-
E BLACK
ORANGE-
AMYLOPECTIN
YELLOW
DEXTRIN VIOLET
REDDISH-
GLYCOGE
BROWN
N
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• The iodine test is qualitative, which is one of its major drawbacks.
• The presence or lack of starch in the sample can be detected by the iodine test, on the
other hand, it cannot be used to quantify the amount of starch present in the sample.
• Another drawback is that starch hydrolysis is inhibited under acidic environments.
As a result, for acidic materials, the iodine test will be invalid.
• The iodine test cannot be done on a sample that is extremely dark in color because
the color changes will be undetectable.
LIMITATIONS 18
CONCLUSION
The Iodine Test is used to determine the presence of polysaccharides, such as starch and
dextrin, in various samples. With the addition of iodine, the presence of starch in a
sample produces a dark blue-colored complex while the dilution of the yellow color of
the iodine indicates the absence of starch. This test can be performed to test the process
of photosynthesis in plants.
On the other hand, the presence of dextrin in a sample produces a violet color with the
addition of iodine. Similar to the test in starch, the dilution of the yellow color of the
iodine indicates the absence of dextrin.
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THANK
YOU!
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REFERENCES
https://
https://
www.youtube.com/
microbenotes.com/
watch?
iodine-test/
v=72zKnccyH-w
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