Medical Skills Practical Revision

2nd year
Faculty of Applied Medical Sciences
31.10.2023
 Section –A
Question No: 1

• Mention the name of laboratory accessories

and mention their use in lab.
a. Name: Fore arm

b. Site of extraction of blood: Median Cubital Vein

c. Mention the various vacuum tubes used in various tests:

 Blue Tube (Citrate): Coagulation studies
 Yellow Tube (SST): All blood chemistry profiles
 Red Tube (Serum): Viral studies
 Purple Tube (EDTA): Hematological studies

d. Uses: Learn to identify the vein for extraction of blood for various laboratory
tests.
e. Demonstrate how to extract blood aseptically:
 Question No: 2
• The Experiment:
• You will use a variable micropipette to prepare
seven different dye mixtures in the wells of a
microtiter plate.
Red ₍µl₎ Blue ₍µl₎ Yellow ₍µl₎ Glycerol ₍µl₎ Alcohol ₍µl₎ Buffer ₍µl₎ Total volume
₍µl₎

6
 Question No:3

a. Name of the lab glassware: Burette

b. Mention the calibration Temperature: 20 ±°C

b.TD refers to: To deliver
c. Mention the meniscus reading in burette:
23.45 mL
d. Uses :
Quantitative chemical analysis to measure the
volume of unknown liquid
 Question No:4
• Mention about the parts of an instrument
1. Eye piece
2. Objective lens 20X, 40X, 100X
3. Stage
4. Slide clip
5. Iris and diaphragm
6. Coarse adjustment
7. Fine adjustment
8. Light source
9. Base
10. Body
 Question No: 5
• Mention the name of laboratory accessories and
mention their uses in lab.

a. Name: Personal protective equipment (PPE)

b. Mention name of laboratory accessories:

Gloves, Mask, safety googles, lab coat and shoes.
c. Uses:
To protect the wearer's body from injury or
infection.
 Question No: 6
• Mention the name of laboratory accessories
and mention their use in lab.
a. Name: Micropipette

b. Mention the parts of laboratory accessories:

c. Uses:
Used to transfer small amounts (< 1 ml) of
liquids.
 Section- B
Question No: 7
a. Name of the lab glassware: Volumetric flask

b. Mention weather its: Volumetric or Non

Volumetric
 Question No:8
a. Name of the lab glassware: Volumetric
transfer pipette

b. Mention its total volume: 10 ml, 20 ml,25

ml,50 ml,100 ml.
 Question No:9
a. Name of the lab glassware: Erlenmeyer flask

b. Mention weather its: Volumetric or Non Volumetric

c. Mention its uses:
Used for preparing titrations and storing stock
solutions
 Question No: 10
a. Name of the lab glassware: Griffin beaker

b. Mention its uses:

Measuring, mixing and decanting tasks in
laboratory
 Question No:11
a. Name of instrument: Water bath

b. Type of thermometer used: Partial immersion

c. Temperature set for working: +5-100°C

 Question No:12
a. Name of instrument: Heating ovens

b. Temperature set for working: 0 - 240oC

c. Type of thermometer used: Partial immersion
 Question No:13
a. Name of instrument: Single Tube Vortex
Mixer

b. Used for: homogenous mixing of liquids.

 Question No:14
a. Name of instrument: Refrigerator

b. Temperature set for working: 4.5-5°C

c. Type of thermometer used: Total immersion

d. Used for: Preservation of specimens at low
temperature for short periods.
 Question No:15
a. Name of instrument: Deep freezer

b. Temperature set for working: -20 to -80°C

c. Type of thermometer used: Total immersion
d. Used for: Preservation of specimens at low
temperature for long periods.
 Question No:16
a. Name: Thermometer

b. Uses: To measure and cross check instrument

temperature
 Question No:17
a. Name of the lab glassware: Volumetric
Measuring cylinder

b. Mention its uses:

Measure accurate volume of liquids.
 Question No:18
a. Name of instrument: Digital rotator

b. Uses: optimal mixing of Ag and Ab reactions.

Ex. VDRL, RPR, other latex tests.
 Question No:19
a. Name of instrument: Infrared Vein finder

b. Uses:
To observe the small veins for extraction blood
samples
 Question No:20
a. Name of instrument: Fire and eye safety shower

b. Uses: protect an employee from injury in case of

contact with hazardous chemicals, chemical
compounds, or fire.
 Question No:21
a. Name of instrument: Fume hood

b. Uses: protect to control inhalation exposures

to hazardous substances
 Question No:22
a. Name of instrument: Biological Spill Kit

b. Uses: to safely clean up small spills of

biological materials.
 Question No:23
a. Name of safety passage: Emergency exit
door

• Uses: safe way to evacuate a building in case

of a crisis or disaster