CATALASE TEST

HEMOPROTIEN
2H2O

PRINCIPLE
AEROBIC CATALASE 2H2O2
BACTERIA
& FUNGUS
O2
 REQUIREMENTS

SPECIMEN
NONGLASS SLIDES
3%BLOOD
H2O2 AGAR TEST TUBES GLASS ROD

PLATES
NUTRIENT AGAR
STORAGE OF
H2 O 2
 1- Transfer pure growth of the organism from the
agar to a clean slide with a loop or glass rod
SLIDE TEST
3 STEPS
2- Immediately add a drop of 3% H2O2 to the
growth
3- Observe for bubble formation

PROCEDURE
1- Take 1ml of 3% H2O2 in 12x100mm test tube
TUBE 2- Introduce small quantity of bacterial growth into
the fluid with the help of a glass rod or plastic loop
TEST & touch the side of the tube
3- Observe the release of bubbles
QUALITY
CONTROL
 RESULTS &
RAPID & SUSTAINED APPEARANCE OF BUBBLES CONSTITUTES A
POSITIVE TEST
IT MEANS BACTERIA POSSESSES THE ENZYME CATALASE, HENCE
CATALASE POSITIVE

INTERPRETATION
SOME BACTERIA POSSESS ENZYMES OTHER THAN CATALASE THAT
CAN DECOMPOSE H2O2. HENCE , FORMING A FEW TINY BUBBLES
AFTER 20-30 SECONDS IS NOT CONSIDERED A POSITIVE TEST
 CATALASE POSITIVE CATALASE NEGATIVE
BACTERIA BACTERIA
Staphylococci Streptococcus pyogenes
Micrococci Gardnerella vaginalis
Corynebacterium diphtheriae Fusobacterium species
Enterobacteriaceae Eikenella corrodens
Listeria monocytogenes Kingella kinge
Shigella dysenteriae type 1
Fatumella ptysees
SEMI-QUANTITATIVE
CATALASE TEST
CATALASE TEST
FOR
HEAT STABLE
MYCOBACTERIA
CATALASE TEST
 Distilled
30% 0.2% Water or
H2O2 Catechol NS

PROCEDURE
+ adding
it to
adding
it to
5ml of
Mycobacterial
test culture

In equal volume
STRONGLY POSITIVE WEAKLY POSITIVE

ATYPICAL MYCOBACTERIA Mycobacterium tuberculosis

Mycobacterium gastri
Thank You