CRYOPRESERVATION

AND ITS BIO-MEDICAL

APPLICATIONS

GROUP MEMBERS

R.EDWIN(2OBME1111)

B.HEMANTH KUMAR(20BME1119)

VIROSHON KINGSLY(
PROBLEM DEFINITION

>Ice Crystal Formation: The problem of ice crystal formation during freezing, which can
cause cellular damage due to mechanical stress and solute concentration effects.

>Cryoprotectant Toxicity: The dilemma of using cryoprotectants to mitigate ice crystal

formation, as some cryoprotectants may be toxic to cells and tissues, affecting their
quality post-thawing.

>Cellular Dehydration: The issue of cellular dehydration during freezing, which can lead to
osmotic stress and structural damage, particularly in sensitive cell types.

>Optimal Cooling and Warming Rates: The need to carefully control the cooling and warming
rates to prevent intracellular and extracellular ice formation and maintain cell and tissue
integrity.

>Heterogeneity of Cell Types: The challenge of preserving diverse cell types with varying
sensitivities to freezing and thawing, requiring tailored cryopreservation protocols.
SOLIDWORKS MODEL

DIMENSIONS-80x60x70(in mm)
PROCEDURE
FOLLOWED

• VELOCITY READING
• SPECIMEN
POSITIONING
• HEAT TRANSFER
COEFFICIENT
CFD MODEL
• GOVERNING EQUATIONS-

• SOFTWARE USED-ANSYS Fluent was the chosen

Computational Fluid Dynamics (CFD) software to
simulate the heat transfer mechanism within
the batch freezer.
ASSUMPTIONS

1. The thermal profile of the injector has been utilised

2. The glycerine bags are all initially set at a

temperature of 17 C.

3. No heat transfer occurs at the walls of the freezer.

4. The presence of liquid nitrogen will not be included

only the gaseous phase.
 MESH DETAILS

LEVEL NUMBER OF CELL SKEWNESS TIME PER

CELLS TYPE ITERATION
Coarse 506,402 HEXAHEDRAL 0.656 5-10s
Coarse 566,030 TETRAHEDRAL 0.701 5-10s
Medium 1,402,790 HEXAHEDRAL 0,736 10-12s
 TEMPERATURE CONTOUR PROFILES

a)Countour profile at 40c b)Countour profile at 50c

Temperature contour profile

c)Contour profile at 60c