Extraction and Purification of Protease from

Culantro, “Awa-phadigom” in Manipur.

Presented By
Kh Sartaj Hussain
• Introduction

Culantro (Eryngium foetidum), local name Awa-phadigom, is a binneil medicinal leafy green
herb belongs to the family Apiaceae. It is commonly grown in moist and shaded areas.
It is found in West Indies and several countries in South America and Asia. In India, it is
found mostly in North-East state of Sakkim (Bhotay dhania), Assam (Man dhania), Tripura
(Bilati dhania), Nagaland (Burma dhania) (Tshering Tashi Lepcha et al., 2018).
Culantro content various nutreints, moisture (83.3%), crude protein (2.63%), sugar (8.26%),
ascorbic acid (32.3 mg/100g), fat (0.73%), ash (3.0 %) (Tshering Tashi Lepcha et al., 2018).
Protease can hydrolyze protein and its activity is already reported from cilantro (coriander).
Culantro is widely used in seasoning a large vareity of foods that includes curries, meats,
soups, etc.
Medicinal uses of culantro:
Treatment of infectious disease
Treatment of diabetes
Treats fever
Detoxification
 Fig: Culantro ( Awa phadigom)

Aim and Objective:

1 Extraction and purification of protease from culantro
2 Applcations of protease in various fields.
Materials and Methods

Materials
Beakers, reagent bottles, conical flasks, plastic bottle , wash bottle, measuring
cylinder of various capacities, centrifuge and digital balance.
Extraction buffer( 0.125M tris HCl), casein, TCA, ammonium sulphate.
Collection of sample : The fresh sample along with roots collect from From
districts of Manipur.
Sample preparation: Leaves are removed, cut and washed.
Methods

• Protein extraction and purification

Culantro leaves Homogenization centrifuge supernatant

( mortar pestle) (crude enzyme)

precipitation centrifuge
crude enzyme assay
(ammonium sulphate)

Ion Exchange chromatography or gel filtration

chromatography
Protease activity test
Protease activity can be determined by measuring the absorbance with
spectophotometer
Applications
Meat tenderization
Anti aging and cosmatic products
Future scope
Study on protease activity, characterizations and applications on various feilds such as
in industries, pharmaceutical, agriculture etc.
Referrences
Ganesan P, Phaiphan AY, Murugan Y, Baharin BS. Comparative study of bioactive compounds in
curry and Coriander leaves an update. Journal of Chemical and Pharmaceutical Research. 2013

Khan SA, Ahmad I, Mohajir MS. Evaluation of mineral contents of some medicinal plants. Pakistan
Journal Pharmceautical Science. 2006

Nadeem M, Anjum FM, Khan MI, Tehseen S, El-Ghorab A, Sultan JI. Nutritional and Medicinal
aspects of coriander (Coriandrum sativum L.): A review, British Food Journal. 2013

Wang P., Su Z., Yuan W., Deng G., Li S. Phytochemical constituents and pharmacological activities of
Eryngium L. (Apiaceae) Pharmaceut. Crops. 2012

 Pino J.A., Rosado A., Fuentes V. Composition of the leaf oil of Eryngium foetidum L. from Cuba. J.
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