ANALYTICAL

TECHNIQUES for
POIOSON ANALYSIS
 INTRODUCTION

Analytical techniques for poison

analysis play a vital role in forensic
toxicology, environmental monitoring,
and clinical settings. These techniques
allow for the identification,
quantification, and characterization of
toxic substances in biological and
environmental samples.
 ANALYTICAL TECHNIQUES
Common analytical techniques for poison analysis are:

1-Chromatography:

Chromatography is a separation technique that

separates different components of a mixture based
on their physical and chemical properties. It is
widely used in poison analysis due to its
versatility, sensitivity, and ability to handle
complex samples.
1.1- Gas chromatography (GC):

GC is a type of chromatography that separates components based on their

volatility and partitioning behavior between a stationary phase and a mobile
phase. It is commonly used for volatile and thermally stable compounds.

1.2- High-performance liquid chromatography (HPLC):

HPLC is a type of chromatography that separates components based on their

polarity and distribution between a stationary phase and a mobile phase. It is
commonly used for non-volatile and thermally labile compounds.
1.3- Thin-layer chromatography (TLC):

TLC is a simple and cost-effective chromatographic technique that is often used for
screening and preliminary identification of poisons. It involves separating components
on a thin layer of absorbent material, such as silica gel or alumina.

2- Mass spectrometry (MS):

MS is an analytical technique that identifies and quantifies compounds based on their

mass-to-charge ratio. It is a highly sensitive and selective technique that can provide
detailed information about the molecular structure of a substance.
3- Immunoassays:

Immunoassays are analytical techniques that utilize antibodies to detect and

quantify specific substances. They are often used for screening and rapid
analysis of poisons.

3.1- Enzyme-linked immunosorbent assay (ELISA):

ELISA is a type of immunoassay that is commonly used for the detection and
quantification of poisons in biological fluids. It is a sensitive and specific
technique that can be automated for high-throughput analysis.
3.2- Lateral flow immunoassay (LFIA):

LFIA is a type of immunoassay that is often used for

point-of-care testing. It is a simple and portable technique
that can be used to detect poisons in urine, blood, or other
biological fluids.
Forensic Toxicology
Forensic toxicology is the application of toxicology to the
investigation of crimes, particularly those involving poisoning.
Analytical techniques play a crucial role in forensic toxicology by
providing evidence of poisoning, identifying the specific poison, and
determining the cause of death.

Key applications of analytical techniques in

forensic toxicology:

• Identifying the cause of death

• Identifying the specific poison
• Determining the route of exposure
 Environmental Monitoring

Environmental monitoring involves assessing the levels of

potentially harmful substances in the environment to protect human
health and ecosystems. Analytical techniques play a vital role in
environmental monitoring by providing accurate and reliable data
on the presence and concentration of toxic substances.

Key applications of analytical techniques in forensic

toxicology:

• Identifying and quantifying pollutants

• Monitoring the effectiveness of environmental regulations
• Investigating environmental contamination incidents
Clinical Setting

In clinical settings, analytical techniques are used to

diagnose poisonings, monitor drug levels, and assess
treatment efficacy. These techniques can help to ensure
the safe and effective use of medications and identify
potential adverse drug reactions.

Key applications of analytical techniques in forensic

toxicology:

• Diagnosing poisonings
• Monitoring drug levels
• Assessing treatment efficacy
Research
Analytical techniques play a critical role in research aimed at developing
new antidotes, improving diagnostic methods, and understanding the
mechanisms of toxicity. These techniques provide valuable insights into the
properties and interactions of toxic substances, leading to better prevention,
treatment, and management of poisonings.

Key applications of analytical techniques in poison research:

• Developing new antidotes:

Analytical techniques are used to identify and characterize potential

antidotes for specific poisons. This information can guide the development
and testing of new antidotes, improving the treatment of poisonings.
• Improving diagnostic methods:

Analytical techniques are used to develop and improve

diagnostic methods for poisoning, such as more sensitive and
specific immunoassays or mass spectrometry applications. This
can lead to earlier and more accurate diagnosis of poisonings.

• Understanding the mechanisms of toxicity:

Analytical techniques are used to investigate the mechanisms by

which toxic substances cause harm at the cellular and molecular
level. This understanding can lead to the development of targeted
therapies and preventive measures.
THANK YOU…