CRUDE DNA

EXTRACTION
SUPERGROUP B
(GROUP 3 & 4)
 ARE YOU
READY FOR THE
NEXT
CHAPTER? DNA
INTRODUCTION
• WHAT IS DNA?
• DNA EXTRACTION?
• PLANT TISSUE?
• DNA EXTRACTION FROM PLANT
TISSUE
• USES OF THE EXPERIMENT DNA
 In the end of the study, reporters
aims to: LAB
OBSERVATION
• Explain the conducted laboratory OBJECTIVES:

experiment.
• Explain the purpose of each step in
the extraction procedures.
• Interpret what happens to DNA and
what it is actually looks like. DNA
Methods
Methods
Result
Methods
Methods
Result
Methods
Observation….…
QUALITATIVE QUANTITATIVE
Room Cold Room Cold
Temp Temp Temp Temp
After 3 minutes… Amount of test tubes
Color Extracted DNA
Layer Time to complete
DNA floats crude DNA extraction
DISCUSSION
WHY DO YOU NEED TO SQUASH OR
CRUSH THE FRUIT?

WHAT IS THE PURPOSE OF LYSATE

SOLUTION IN THE EXTRACTION OF DNA?

HOW DOES THE DISHWASHING LIQUID

AND TABLE SALT HELP IN THE
EXTRACTION OF DNA?
DISCUSSION
WHY DO YOU NEED TO
SQUASH OR CRUSH THE
FRUIT?

WHAT IS THE PURPOSE OF LYSATE SOLUTION IN THE EXTRACTION OF DNA?

HOW DOES THE DISHWASHING LIQUID AND TABLE SALT HELP IN THE
EXTRACTION OF DNA?
DISCUSSION
WHY DO YOU NEED TO SQUASH OR CRUSH THE FRUIT?

WHAT IS THE PURPOSE OF

LYSATE SOLUTION IN THE
EXTRACTION OF DNA?

HOW DOES THE DISHWASHING LIQUID AND TABLE SALT HELP IN THE
EXTRACTION OF DNA?
DISCUSSION
WHY DO YOU NEED TO SQUASH OR CRUSH THE FRUIT?

WHAT IS THE PURPOSE OF LYSATE SOLUTION IN THE EXTRACTION OF DNA?

HOW DOES THE DISHWASHING

LIQUID AND TABLE SALT HELP IN
THE EXTRACTION OF DNA?
DISCUSSION
WOULD YOU BE ABLE TO EXTRACT DNA IF THE
TISSUE-LYSATE SOLUTION WAS MIXED USING
A FOOD PROCESSOR/BLENDER?

WHAT IS THE DIFFERENCE WHEN COLD

ETHANOL OR ETHANOL AT ROOM
TEMPERATURE WAS USED IN THE
EXPERIMENT?

ENUMERATE THREE (3) USES OF

EXTRACTED DNA.
DISCUSSION
WOULD YOU BE ABLE TO
EXTRACT DNA IF THE TISSUE-
LYSATE SOLUTION WAS MIXED
USING A FOOD
PROCESSOR/BLENDER?
WHAT IS THE DIFFERENCE WHEN COLD ETHANOL OR ETHANOL AT ROOM
TEMPERATURE WAS USED IN THE EXPERIMENT?

ENUMERATE THREE (3) USES OF EXTRACTED DNA.

DISCUSSION
WOULD YOU BE ABLE TO EXTRACT DNA IF THE TISSUE-LYSATE SOLUTION
WAS MIXED USING A FOOD PROCESSOR/BLENDER?

WHAT IS THE DIFFERENCE WHEN

COLD ETHANOL OR ETHANOL AT
ROOM TEMPERATURE WAS USED
IN THE EXPERIMENT?

ENUMERATE THREE (3) USES OF EXTRACTED DNA.

DISCUSSION
WOULD YOU BE ABLE TO EXTRACT DNA IF THE TISSUE-LYSATE SOLUTION
WAS MIXED USING A FOOD PROCESSOR/BLENDER?

WHAT IS THE DIFFERENCE WHEN COLD ETHANOL OR ETHANOL AT ROOM

TEMPERATURE WAS USED IN THE EXPERIMENT?

ENUMERATE THREE (3) USES OF

EXTRACTED DNA.
After the analysis of the results
obtained;
• Materials Conclusion
• Solution
• Temperature
• Crude DNA extraction
• Uses
Reference……
• Conte, S. (n.d.). Experiment: extraction of DNA from a tomato. prezi.com. https://prezi.com/5hwgwou1m5hk/experiment-
extraction-of-dna-from-a-tomato/#:~:text=What%20can%20we%20conclude%3F,the%20cells%20of%20the%20tomato.
• Activity 1 - DNA extraction. (n.d.). Activity 1 - DNA Extraction.
https://www.apsnet.org/edcenter/disimpactmngmnt/labexercises/PlantBiotechnology/Pages/Activ ity1.aspx
• Budowle, B., Eisenberg, A. J., & van Daal, A. (2009). Validity of low copy number typing and applications to forensic science.
Croatian Medical Journal, 50(3), 207-217.
• Butler, J. M. (2015). Advanced topics in forensic DNA typing: methodology. Academic Press. Jobling, M. A., & Gill, P. (2004).
Encoded evidence: DNA in forensic analysis. Nature Reviews Genetics, 5(10), 739-751.
• Center, S. L. (2021, December 13). Extract DNA at home! Lemelson Center for the Study of Invention and Innovation.
https://invention.si.edu/extract-dna-home
• Green, R. C., Berg, J. S., Grody, W. W., Kalia, S. S., Korf, B. R., Martin, C. L., ... & Biesecker, L. G. (2013). ACMG
recommendations for reporting of incidental findings in clinical exome and genome sequencing. Genetics in Medicine, 15(7),
565-574.
• International Human Genome Sequencing Consortium. (2004). Finishing the euchromatic sequence of the human genome.
Nature, 431(7011), 931-945.
• Manolio, T. A., Collins, F. S., Cox, N. J., Goldstein, D. B., Hindorff, L. A., Hunter, D. J., ... & Cho, J. H. (2009). Finding the
missing heritability of complex diseases. Nature, 461(7265), 747- 753.
• National Human Genome Research Institute. (2018). Genomics and Medicine. Retrieved from
https://www.genome.gov/27568501/genomics-and-medicine/
• Richards, S., Aziz, N., Bale, S., Bick, D., Das, S., Gastier-Foster, J., ... & Lyon, E. (2015). Standards and guidelines for the
interpretation of sequence variants: a joint consensus recommendation of the American College of Medical Genetics and
Genomics and the Association for Molecular Pathology. Genetics in Medicine, 17(5), 405-424.
• Visscher, P. M., Brown, M. A., McCarthy, M. I., & Yang, J. (2012). Five years of GWAS discovery. American Journal of Human
Genetics, 90(1), 7-24.
• What Is the Purpose of a Lysis Solution in DNA Extraction? (n.d.). Quora. Retrieved October 7, 2023, from
https://www.quora.com/What-is-the-purpose-of-a-lysis-solution-in-DNAextraction/answer/Sourav-Pan-17
Reference……
• Alaska BioPREP Virtual Textbook. (n.d.). The basics of DNA extraction. University of Alaska
Fairbanks.
• Bio Technology Notes. (n.d.). 3 main steps involved in the extraction of DNA.
• ScienceDirect. (2019). DNA extraction - an overview
• Short-Fact ( 2020, May 1) General :What is the role of salt and detergent in DNA extraction?
https://short-fact.com/what-is-the-role-of-salt-and-detergent-in-dna-extraction/
• Promega (2023) DNA Purification : DNA extraction method
https://worldwide.promega.com/resources/guides/nucleic-acid-analysis/dna-purification/
• Revolutionary Science (2023) Common Uses for DNA Extraction for Labs
https://www.revsci.com/blogs/biotechnology/common-uses-for-dna-extraction
 Remember…
Curiosity First!
Always keep on learning because life doesn’t stop
teaching