Image session

Microbiology

2 nd
year MBBS
27.10.22
Reagents………
Reagents………
 GRAM STAINING

Gram Positive
Appears purple/blue

Gram negative

Appears pink
GRAM STAINING
Streptococcus Staphylococcus
-Gram positive cocci -Gram positive cocci
- Arrange in chains - Arrange in clusters
Gram stain of S. pneumoniae
with WBC

*May occur intracellularly

or extracellularly

*Gram-positive,diplococci

*Sometimes occurring in
short chains
GRAM NEGATIVE COCCI
GRAM NEGATIVE RODS

Escherichia coli
 SPORES
Bacillus anthracis (Gram staining)
 Cells have characteristic
squared ends
 endospores are ellipsoidal
shaped
 centrally located
 resistant to staining
SPORES
 Z N STAINING
Acid fast bacilli
Straight/curved rods
pink in colour
PUS CELLS
EPITHELIAL CELLS
 CULTURE MEDIA
nt agar

agar
A

nkey

C B
Growth on Sheep Blood Agar
Types of Hemolysis on Blood Agar
CHOCOLATE AGAR
MacConkey Agar
 MacConkey Agar

Non-Lactose fermenting colonies Lactose fermenting colonies

Blood Culture
TRIPPLE SUGAR IRON AGAR
(TSI AGAR)
TRIPPLE SUGAR IRON AGAR
(TSI AGAR)
 CLED AGAR
1. Lactose fermenting colonies

2. Non-Lactose fermenting colonies

Sensitivity plate with drug discs
(Mueller Hinton Agar)
ANALYTICAL PROFILE INDEX (API)
CATALASE TEST
COAGULASE TEST
NOVOBIOCIN SENSITIVITY TEST
 OXIDASE TEST
A. Positive oxidase test

B. Negative oxidase test

10-99 AFB in 100
100 fields
fields
Immunochromatographic assays/ICT
 SAMPLE COLLECTION

High Vaginal Swab (HVS)

Pus collection container, and swab

 SAMPLE COLLECTION

Urine collection container

Sputum collection containers

SAMPLE COLLECTION

CSF collection container

Diseases caused by staphylococcus aureus

Impetigo Paronychia

Stye

Wound infection Abscess

STAPHYLOCOCCAL SCALDED SKIN SYNDROME

• Ritter’s
: disease(severe form)
IN NEW BORN • Pamphigus
neonatorum
(milder form)

• Toxic epidermal
necrolysis(severe
IN OLDER form)
PATIENTS • Bullous
impetigo(milder form)
• Toxic Shock Syndrome Toxin
(TSST)
Cellulitis –caused by gram positive
streptococcus pyogenes
• Identify the given medium
• What is the use of this medium?
• What type of medium is this?
• Write down its contents
• Look at the photograph and answer the following
questions
• Which type of organism is this?
• Give 2 morphological features
• What biochemical test will you perform to confirm
the diagnosis
• Write down the principle of given biochemical test.
• Identify the given devise used in serological
technique
• Name the serological technique in which it is used.
• Give 2 clinical applications of this technique
W I S H YO U B EST O F LU C K