Fluorescence

Spectroscopy
AYA BENDAHMAN
GHASSANE SIRAJ SANI
 Summary:
1. What is the phenomenon of Fluorescence?
2. How does fluorescence spectrometry work ?
3. What are it’s pros and cons?
4. Some of its implication and utilities.
 Fluorescence
The emission of electromagnetic waves,
(luminescence) caused by the excitation of
atoms or nanostructure, which they re-
emit immediately (with-in 10^-8 s).
 Phosphorescence
Another type of luminescence that is
caused by the absorption of radiations,
and that continues for noticeable amounts
of time. Even after the radiation source
stops.
 The commun point between
fluorescence and phosphorescence
is the release of photons while the
electrons return to the ground
state.
However they differ in the
duration in which they do it.
 The actual difference between them:

Fluorescence
When the atom relaxes to
the ground state (and emits
photons) without any
change in electron spins.
Phosphorescence
there is a change in electron
spin, which results in a
longer lifetime of the
excited state (second to
minutes).
 Re-cap

Absorption Excitation State Re-emission

Of the radiation’s Where the electron’s energy Where lies the difference
energy levels change. between the two
E = h*f. phenomenons.
8
 Luminescence!
01 SpECTROSCOPY
Mechanism and utilities
 Fluo/phosphorescence
spectroscopy

➢ Photoluminescence spectroscopy is a contactless, nondestructive

method of probing the electronic structure of materials.
It consists of the study of UV, visible, and near-infrared
(NIR) light that is emitted by a chemical species after having
absorbed light.
 DAY 1
Preparation of
Samples

Luminescence
spectroscopy
procedures
DAY 2
Exposure &
Re-emission
 3
Spectrum
analysis
Limitations of fluorescence Measurements

Concentration
Solvent effects effects
Background Interfering Inner filter
effects due to nonspecific effects,
light scattering fluorescence concentration
quenching

Sample effects
Light scattering,
interfering
fluorescence, Here you could
sample adsorption talk about this
person
 Avdantages of fluorescence
spectroscopy
SENSITIVITY:
•It is more sensitive as concentration is low as ug/ml or ng/ml.
PRECISION:
• Upto 1 % can be achieved.

SPECIFICITY:
• More specific than absorption method where absorption
maxima may be same for two compounds.

RANGE OF APPLICATION:
Even non fluorescent compounds can also be converted to
fluorescent compounds by chemical compounds.
 DisAvdantages of fluorescence
spectroscopy

 Not really useful for identification

 Not all compounds fluorescence
 Contamination can quench the
fluorescence and hence give false/no
results
 Applications of luminescence spectroscopy

01 Analytical Chemistry

04 Pharmacy 02 Environmental studies

05 Medecine 03 Biochemistry
Thank you for your
attention