GSCI 2013: Group 2-Cell and

Developmental Biology
Seth Jones, University of Kentucky
Karen Maruska, Louisiana State University
Joy Davis, Baton Rouge Community College
Sabrice Guerrier, Millsaps College
Naila Mamoon, Millsaps College
Facilitator: Peter Cavnar, University of West
Florida
CELLULAR
RESPIRATION
 Context
What kind of course is unit designed for?
mid-level

How long is unit?

1 week

When will the unit be used in the course? After

students have learned basic chemistry,
energetics, redox rxn, protein structure, cell
membrane, transport, and organelles

Class size? Should be fine for all ranges

 Learning goal
• Understand how the energy from electrons in glucose is
converted to chemical energy in ATP during oxidative
phosphorylation.
 Learning outcomes
• Explain the role of oxygen in cellular respiration

• Explain how the H+ gradient across the inner mitochondrial

membrane is generated and how it drives ATP synthesis

• Predict the effects of various drugs on oxidative

phosphorylation
How long can you hold your breath?
Brainstorm: So why do you think we
need oxygen?
Loss of hydrogen
atoms
(becomes oxidized)
C6H12O 6 O2 6 CO 6 H2 AT
6 2 O P
Glucos 
e Gain of hydrogen Heat
atoms
(becomes reduced)
CYTOPLAS
M
NAD
H
Electrons NAD FADH
carried by H 2
NADH

Glycolysi Oxidative
Pyruvate Phosphorylation
Glucos s Pyruvat Citric
Oxidatio Acid (electron
e e
n Cycle transport
and
chemiosmosis)

Mitochondri
on

AT
AT AT P
P P
Substrate- Substrate- Oxidative
level level phosphorylati
phosphorylati phosphorylati on
on on
Figure 6.10

H H H H
H
Interme Protein H Mobile H
m- electro H

H ATP
complex n
brane of synthas
space carriers
electron III e
carriers IV
I

Inner
mito- II
chondrial
membran FAD
e Electro FADH2
n 1
2 H2O
flow NAD NAD
H 2 O2
H
Mito- H
chondri
al AD P AT
matrix P H P

Electron Transport Chemiosmos

Chain is

Oxidative
Phosphorylation
 Welcome to Club Mitochondria
• Instructions: Pair with a
partner then identify the
characters within the
analogy that correspond
to the following:

• 1. Electrons
• 2. Protons
• 3. Electron transport chain (ETC)
• 4. ATP synthase
• 5. Mitochondrial Matrix
• 6. Intermembrane space
What is the layout in Club Mitochondria?
Main entrance

Female Patron

Foyer

Dance
Floor Male Patron
Consider a scenario where the electron transport
chain ("dance line") is disrupted in the
mitochondria.

What is most likely to happen to the H+

concentration ([H+]) in the intermembrane space?

• A. Decrease in [H+]
• B. Increase in [H+]
• C. No change
• D. I don't know
Consider a scenario where the electron transport
chain ("dance line") is disrupted in the
mitochondria.

What change would you expect in ATP

concentration in the cell?

• A. Decrease in [ATP]
• B. Increase in [ATP]
• C. No change
• D. I don't know
Figure 6.11

Inhibitors of cellular respiration

Rotenon Cyanide, Oligomyci

H
H e carbon n
H
monoxide ATP
H
synthas
H H H e

DNP

FADH FA
D
2
1
2 H
NAD NAD 
2 O2
H
H
Your homework: List the signs and symptoms in H2 AD P AT
victims with acute cyanide poisoning AND we O P P
would like you to extend the analogy to include
oxygen and ATP