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Biomolecules Class 12
Biomolecules Class 12
PART-I
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Objectives
After studying this Unit, you will be able to:
explain the characteristics of biomolecules like
carbohydrates, proteins and nucleic acids and
hormones;
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Your chapter is cover the following major content :
Carbohydrates
Proteins
Enzymes
Vitamins
Nucleic Acids
Hormones
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Carbohydrates : Carbohydrates may be defined as optically
active polyhydroxy aldehydes or ketones or compounds which produce
such units on hydrolysis. e.g., cellulose, glycogen, starch, etc.
starch
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Some of the carbohydrates, which are sweet in taste, are also called
sugars.
The most common sugar, used in our homes is named as sucrose
whereas the sugar present in milk is known as lactose.
Carbohydrates are also called saccharides (Greek: sakcharon
means sugar).
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sucrose
14.1.1 Classification of Carbohydrates
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Polysaccharides are not sweet in taste, hence they are
also called non-sugars.
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Sugar : In general, monosaccharides and oligosaccharides
are crystalline solids, soluble in water, sweet in taste.
They are collectively called sugars. e.g., glucose,
fructose, sucrose etc. They are of two types :
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14.1.2Monosaccharides
Some monosaccharides with example are given in the table as shown below :
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14.1.2.1 Glucose
Glucose occurs freely in nature as well as in the combined form. It is present in sweet fruits
and honey. Ripe grapes also contain glucose in large amounts.
Preparation of Glucose
1. From sucrose (Cane sugar): If sucrose is boiled with dilute HCl or H2SO4 in
alcoholic solution, glucose and fructose are obtained inequal amounts.
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Structure of Glucose
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The exact spatial arrangement of different —OH groups was
given by Fischer after studying many other properties. Its
configuration is correctly represented as I. So gluconic acid
is represented as II and saccharic acid as III.
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Glucose is correctly named as D(+)-glucose.
‘D’ before the name of glucose represents the
configuration, whereas ‘(+)’ represents
dextrorotatory nature of the molecule.
It should be remembered that ‘D’ and ‘L’ have no
relation with the optical activity of the compound.
They are also not related to letter ‘d’ and ‘l’.
The meaning of D– and L– notations is as follows in
next slide.
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Cyclic Structure of Glucose
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Among the facts that had still to be accounted for were the following:
(a) D-(+)-Glucose fails to undergo certain reactions typical of aldehydes. Although it is readily
oxidized, it gives a negative Schiff test and does not form a bisulfite addition product.
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Schiff’s Test Mechanism
The bisulfite and para-rosaniline react together to afford a decolourised adduct whose
central carbon is sulfonated. Now, the free & uncharged amine groups belonging to the
aromatic ring that react with the aldehyde group to form an aldimine. This aldimine group
is an excellent electrophile and, therefore, undergoes further reaction with the bisulfite
ion. Finally, a purple or magenta coloured bisulfite adduct is formed.
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(b) The pentaacetate of glucose does not react with hydroxylamine
indicating the absence of free —CHO group.
(c) Glucose is found to exist in two different crystalline forms which
are named as a and b. The a-form of glucose (m.p. 419 K) is obtained
by crystallisation from concentrated solution of glucose at 303 K while
the b-form (m.p. 423 K) is obtained by crystallisation from hot and
saturated aqueous solution at 371 K.
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The two cyclic hemiacetal forms of glucose differ only
in the configuration of the hydroxyl group at C1,
called anomeric carbon (the aldehyde carbon before
cyclisation). Such isomers, i.e., a(alpha) form and
b(beta)-form, are called anomers.
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14.1.2.2 Fructose
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Structure of Fructose
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DISACCHARIDES
The two monosaccharides are joined together by an oxide linkage
formed by the loss of a water molecule. Such a linkage between
two monosaccharide units through oxygen atom is called
glycosidic linkage.
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