General detection-UHPLC product series

introduction + maintenance

— 2023 —

www.chromai.com

Copyright:Chromai (Beijing) Technology Co., Ltd

目 录
01 Company

02 Leaps Product

03 Single Module

04 EyouLab CDS

05 Typical Application Cases

06 6
Maintenance
公司简介

Innovation for Better life

 Chromai

• Chromai is a high-tech enterprise engaged in the R&D, production, sales and service of analytical testing instruments
Company Profile
and medical diagnostics. It is a member of China Instrument and Meter Association and China Medical Device
Industry Association. The company focuses on the full combination of scientific instruments, automation and
intelligence, and serves the fields of analysis and testing and medical testing.
• The company insists on independent innovation and takes the revitalization of national industry as its own
responsibility. The vision is to fundamentally change the global industry market structure, make China's creations
famous all over the world, and build Konomi into an outstanding international brand.
 Chromai

Business Direction • Chromai adheres to independent innovation and takes the revitalization of national industries as its
mission. Its vision is to fundamentally change the global industry market structure, make China's
creations famous all over the world, and build Chromai into an outstanding international brand.
Fundamentally break the import monopoly, improve efficiency, reduce costs, and create value for
customers. The company now has Beijing R&D center, Suzhou production base and other
subsidiaries.

• At present, it is mainly aimed at the two fields of general testing and precision medicine:

• General detection field: Leaps series ultra-high performance liquid chromatography system, Pump,
Autosampler, EyouLab CDS and related components and consumables, etc.

• Precision medicine field: Voyager series IVD two-dimensional ultra-high performance liquid
chromatography system, EpoStar series automatic sample processing platform, exclusive
methodology-related reagents, etc.
Company Location

BEIJING SUZHOU
Operation Center And Researchcentre Production Factory
Awards
technology lead

UHPLC
Ultra High Efficiency Columns lower flow rate
vs.
HPLC  1.7μm~5μm can be  Use a lower flow rate
 0.001~10.00mL/min
selected
 Choose a smaller  The amount of reagent

particle size column consumed per unit

time is reduced
Higher withstand voltage
 wider pressure range
 10500~16000PSI
 72Mpa~110Mpa
faster
Less solvent loss
 Diffusion rate is faster ,
 single sample analysis
 Get out of the peak
 Solvent costs
faster,
reduced by more
 greatly shorten the
than 50%
time

Comprehensive cost is lower

 Improved analysis efficiency
 Decrease in the amount of reagent used
 Comprehensive cost is greatly reduced
erformance Liquid Chromatography ( UHPLC ), Conventional High Performance Liquid Chromatography ( HPLC )
Application field
  Adapt to different  Implement all  Meet different
experimental application budget needs
scenarios scenarios

Leaps UHPLC Leaps 2D Leaps Bio Leaps Prep

Frontier
Economical and Efficient for all Enabling Efficient Simple and flexible A New Benchmark
durable quality separation challenges Biomolecular Analysis preparation and in Efficiency with
choice purification Ultra-High
Performance
Economical and High precision and Leaps Bio system is Leaps Prep realizes a It has realized a leap in
durable, reasonable good stability. It can made of biologically truly fully automatic the analysis efficiency of
price, stable quality. It efficiently analyze inert materials, which preparative system, liquid chromatography. It
is the best choice to more complex are corrosion- from automatic is a new generation of
reduce project samples to meet the resistant and reduce sampling to automatic ultra-high performance
investment and analysis habits and the adsorption of fraction collection, liquid chromatography
quickly obtain return analysis needs of biological from control operation that has been fully
on investment. more scenarios. macromolecules, to analysis. upgraded, and has set a
effectively ensuring new benchmark for
the durability of the domestic liquid
instrument and the
EyouLab CDS quantitative
chromatography.

repeatability without
loss of samples.
Leaps UHPLC

Column oven
Accurate temperature control,
UVD , DAD detector
optional double valve switching
ELSD , RID , FLD

EyouLab CDS
Regulatory Compliance, Database,
Autosampler
Web Edition
Refrigeration ( +4 ℃ - room data acquisition box
temperature) dual channel

High pressure infusion

pump
isocratic, binary , quaternary,
double ternary
Leaps UHPLC
Economical and Durable 10500 PSI
Quality Choice
Pressure Delivery
Beyond Conventional
The cost-effective NO.1 of self-made LC

Ultra-efficient grade resolution and durability

108 & 192 Vials
Go beyond the routine to ensure reliable analysis

Value-added experience helps Efficient sample delivery

and analysis
you achieve rapid analysis

0.1℃

Accurate and fast temperature

control system
 Simplify the pre-processing to double the analysis efficiency

Leaps 2D
Multi-column switching, perfect determination of complex samples
Complex Analysis
Optional matching, perfect compatibility
One Step Faster
Two-dimensional separation, doubling the peak capacity
Easy determination, greatly reducing pre-treatment
Ultra-efficient grade, higher analysis efficiency

Unlock more complex application scenarios

Analytical application in
√ natural pharmaceutical ingredients
√ Chinese herbal medicine extract components
√ quality control of traditional Chinese medicine
√ toxic and harmful substances in the environment
√ differential proteomics
√ biopharmaceutical analysis
Leaps Bio
Enabling Efficient Biological Sample Analysis
Bioinert material, corrosion
and adsorption resistant
Bio-inert sample flow path

PEEK, titanium and other biocompatible materials

Easily adapted to biological and extreme pH applications

Easily adapted to extreme
Minimizes losses due to metal adsorption conditions

Ensure the stability and reliability of

biological analysis
Flexible application collocation
to ensure stability and reliability
Leaps Bio-HPLC

No adsorption

Higher peak height

More realistic area

wash
pump

1-14 PH
 Infusion systems for high pressure preparation

Leaps Prep
20-40Mpa
Simple and Flexible Preparation
and Purification
A truly fully automated preparative system

From injection to fraction collection, from operation to analysis

5000μL
Help you solve various semi-preparation and preparation applications

Support extension to high pressure 2D system Large-volume injection and

robust separations
High efficiency separation
and column switching function
Solve problems more efficiently

150mL/min

Fully automated fraction collection

Leaps Prep-HPLC
 Extraordinary
Frontier analytical efficiency experience

A New Benchmark in Efficiency

with Ultra-High Performance

16000 PSI

Establish a new benchmark for domestic LC ；

Higher performance, higher efficiency,

Higher level sample delivery
higher price-quality ratio ；

Ultra-high pressure-withstanding capacity

greatly improves analysis efficiency

54*4

A new generation of ultra-high performance liquid chromatography Flexible for more application expansion
 online Man-machine
version separation
EyouLab CDS

Comprehensive Data Security and Regulatory Compliance

Improve your experimental efficiency in an all-round way

The client and the collection end

run independently.
Flexible network deployment is possible.
Frontier UHPLC

Data security
The network
connection is
abnormal. The server
is faulty

Data save
Acquisition local
control terminal
Network
recovery
Server
recovery
Data
synchronization
Data is database server
automatically
saved locally
The network
connection is
normal. The server
is normal
Leaps UHPLC – Pump

Leaps Pump
High pressure infusion pump
Isocratic pump
Binary pump
Binary four-channel pump
Quaternary pump
Double ternary gradient pump
Leaps UHPLC – Pump

 Micro pump type high pressure

constant flow pump P10
 Binary High Pressure Gradient
Pump
 Quaternary Gradient Pump
 Double ternary gradient pump
 Leaps UHPLC – Pump

 isocratic pump
 Binary High Pressure Gradient
Pump
 Quaternary Gradient Pump
 Double ternary high pressure mixer
gradient pump
Vent valve Solvent switching valve

switch

Peristaltic pump
two channels
Degasser

20% methanol / isopropanol in water B pump A pump

 Leaps UHPLC – Pump

 isocratic pump
 Binary High Pressure Gradient
Pump
 Quaternary Gradient Pump P40
 Double ternary gradient pump mixer

Vent valve

switch

Peristaltic pump four channels

Degasser

20% methanol / isopropanol in water Quaternary Gradient Valve

 Leaps UHPLC – Pump

 isocratic pump
 Binary High Pressure Gradient
Pump
 Quaternary low pressure gradient
pump
 Double ternary high pressure
gradient pump
Leaps UHPLC – Pump

Infusion module high pressure UHPLC high-pressure infusion system specification design, using high-pressure
seal
sealing technology to ensure long-term stability and durability
Rinse after standard
configuration
10500 Guaranteed longer life
PSI

Even
durable

Jeweled plunger
rods are more
Sealing ring with
resistant to wear
high pressure
resistance
Leaps UHPLC – Pump

Infusion module higher

efficiency 10500PSI pressure range, bringing more separation conditions and higher
separation efficiency than conventional HPLC

10500
PSI

high
efficiency
40min

Leaps

15min
Leaps UHPLC – Pump

lower
Infusion module pulsation Patented adaptive pulsation suppression technology algorithm,
comprehensively reduces pressure pulsation during infusion

5
PSI

Low
pulsation

Pressure pulsation <

5PSI
Leaps UHPLC – Pump

better
Infusion module gradient Better gradient precision, accuracy and repeatability

RSD
0.2%

high
reproduce

Accuracy 0.50%
Repeatability 0.20%
Leaps UHPLC - Sampler

Leaps
7725i
Autosampler

 The role of the injector: to transport the sample

into the flow system
 Classification of injectors: 1. Manual injectors
2. Autosampler
Leaps UHPLC - Sampler
handle

Leaps Fixing bolts

7725i
 Injection method: importer
Diaphragm injection, stop-flow injection,
valve injection
graphite
rotor
 advantage:
Simple structure, easy maintenance,
cheap price ceramic
stator

 shortcoming:
Body fixing
Low efficiency, artificial injection bolts
is necessary, poor precision
Leaps UHPLC - Sampler

Leaps
7725i

Position 1 ( LOAD ) Position 2 ( INJECT )

Leaps UHPLC - Sampler

5μL
Leaps 10μL
Standard 20 microliters
7725i
20 μL other optional
Quantitative loop
50μL
S316/PEEK
100μL
200μL
500μL
1000μL
5000μL
Leaps UHPLC - Autosampler

Leaps
Autosampler
Leaps UHPLC - Autosampler

Autosampler module

automation
Stable operation
Low residue
High precision
Automation
High volume
Cooling function
Leaps UHPLC - Autosampler
Leaps UHPLC - Autosampler
Leaps UHPLC - Autosampler

High-precision syringe drive unit to achieve excellent

injection accuracy and linearity
Leaps UHPLC - Autosampler

extremely small Patented sampling needle design, ultra-precise polishing of inner

residual and outer walls, automatic cleaning of inner and outer needles,
greatly reducing sample residual pollution
Leaps UHPLC - Autosampler

various
bottle rack
Flexible vial rack options
Leaps UHPLC – Column Oven

Leaps
Column Oven Uniform internal
temperature

1. Temperature control accuracy

2. Large space
3. Uniform temperature
4. Temperature time gradient mode
5. Refrigeration
6. Column switching
7. Alarm functions such as liquid leakage
and over temperature
Leaps UHPLC – Column Oven

more accurate
temperature
Intelligent PID temperature control algorithm, patented forced air circulation
design, the internal temperature field is more uniform; the temperature is more
accurate up to ±0.1°C
60
59.9
65 59.8

Temperature (Celsius)
59.7
59.6
60 59.5
59.4

Temperature (Celsius)
59.3
55
59.2
59.1
50 59
500 550 600 650 700 750 800

time (seconds)
45

40

35
0 600 1200 1800 2400 3000 3600

time (seconds)
Leaps UHPLC – Column Oven

Large space, four 300mm chromatographic columns ; optional double

bigger valve design, convenient for multi-dimensional chromatography
capacity

Multi-Column Switching System

8-12 bits optional

Column Holder

high sensitivity
Temperature Sensor
internal circulation fan

Multi-Column Switching
System
Leakage alarm
Leak Tray
Leaps UHPLC – Column Oven

Chromatographic Provide general-purpose and special-purpose chromatographic

column columns with excellent reproducibility and better separation effect
technology
Leaps UHPLC – Detector

Leaps
UV-Vis Detector
Leaps UHPLC – Detector

100 Sampling rate up to 100Hz , restoring true and accurate

Hz chromatographic peaks beyond conventional HPLC

High sampling rate restores

true peak shape
Leaps UHPLC – Detector

UV-Vis

Lambert - Beer Law :

A − Absorbance
T − transmittance
ε − molar absorptivity
C − Concentration
L − optical path length
Leaps UHPLC – Detector

UV-Vis
Qualitative Analysis :
 Known conditions :
time
The characteristic absorption peak of a certain substance is known, that is, ε (λ) is
known, and it is necessary to judge whether the substance exists .

Quantitative analysis : area

 Known conditions :

It is known that there is a certain substance, that is, ε (λ) is known, and the test
optical path L is fixed, the concentration C of the substance should be tested .
Leaps UHPLC – Detector

Sampling rate up to 100Hz , restoring true and accurate

chromatographic peaks beyond conventional HPLC

re

High sampling rate for

better resolution
Leaps UHPLC – Detector

UV-Vis
Patented optical structure design ensures excellent signal-to-noise
ratio, and lower dynamic noise and drift

lower noise
± 0.5×10 -5 AU
Leaps UHPLC – Detector

Leaps
DAD Detector

• 2uL total reflection pool, 1024 pixel array (full spectrum scanning)
• 10mm high sensitivity flow cell
• 60mm high sensitivity flow cell ( ×6 times)
• 90mm high sensitivity flow cell ( ×9 times)
 Leaps UHPLC – Detector

Leaps flow cell Optical

DAD light
selective system
principle source absorption splitting

Chromato convert
Imaging
graphy, optical
Diode
spectrum signal to
Array
data electrical
Detector
processing signal
Leaps UHPLC – Detector

Leaps
DAD
optical path
 Leaps UHPLC – Detector

ps

e-dimensional map
Leaps UHPLC – Detector

DAD detector UV detector

The tested sample has UV absorption

common points
Follow the Lambert - Beer law to detect the absorbance of the measured sample

The light of all bands passes through the flow cell,

After splitting, the light of a specific wavelength
after being absorbed by the sample in the flow cell, it
enters the flow cell,
is spectroscopically split, and finally received by the
incident on a single detector
linear array sensor
Output time - wavelength - absorbance 3D Output a time - absorbance two-dimensional
difference
chromatogram chromatogram at a specific wavelength

Output chromatographic data at

any wavelength Output spectral data at
Spectrum scan not possible
any time Qualitative analysis: select the best test
wavelength; peak purity identification
Leaps UHPLC – Detector

Leaps 3D spectrum monitoring

DAD , peak purity calculation
function
Comprehensive advantage
Slit width 1.2nm , 8nm Simultaneously monitor
optional, 12 channel wavelengths,

Reference wavelength to
The maximum optical
eliminate background
path is 90mm , realizing
excellent interference, drift
correction function
9X signal sensitivity

1024 Pixels , providing Ultra-high sensitivity,

a wavelength range of low dispersion total
190~800nm ; reflection flow cell

Optical path length

matching ultra-efficient
analysis requirements
Leaps UHPLC – Detector

Leaps
DAD
Comprehensive advantage

60mm

10mm
Leaps UHPLC – Detector
 Leaps UHPLC – Detector

eaps
AB 10
ual channel FLD
ata acquisition box Detector

RID
Detector

ELSD
Detector
EyouLab CDS

• Powerful data analysis and processing, up to 26

integral functions and calculation methods

• Extremely simple user interaction, more friendly

normalized graphical interface , easy to use

• Massive application method database, which can

accommodate > 100,000 methods to improve efficiency

• Intelligent multi-machine joint control, more scalable

LC / GC / IC / LC -MS , etc., flexible expansion

• C ou d cloud service version, visual kanban, easy

remote management of the network

• Fully complies with the applicability requirements of F D

A 2 1 CFR P a r t11 / GMP / G L P and other regulations
EyouLab CDS
EyouLab CDS
☆ EyouLab CDS : Intelligent Chromatography Data Sy
EyouLab CDS

☆ EyouLab CDS
EyouLab CDS
 Enterprise cloud
service
Eliminate IT infrastructure
construction, operation and
maintenance equipment and
personnel to invest in the cloud
platform to help you manage data,
rest assured that massive data
storage can be expanded at any
time to build a secure private cloud
 ODM integration
 easy to use Chromai SDK tool integration
framework, open IIF development
Easy to use Extremely simple user
kit, based on Chromai advanced
interface, easy to use and
chromatography software
convenient
technology,
Can be used in LC, GC, IC, LC-AFS,
LC-MS/MS and other detection
equipment to jointly build a
chromatographic software
ecosystem and provide more
support for third-party instruments

 compliance
Compliant with FDA 21 CFR part
11/GMP/GLP...Network database
 Cloud expert version & cloud service version file
method library system can safely store the database
version, effectively ensuring data
Built-in more than security Automatic backup Electronic
100,000 methods signature audit trail User rights
Massive method management IQ/OQ/PQ
resources to share with  Powerful
thousands of users,
communicate and Rich in functions, everything is available
discover your value Developed based on mainstream database technology
platform
Compatible with various LIMS and HIS systems
EyouLab CDS
Typical Application – Determination of Panax
Ginsenosides in Ginseng Red Ginseng
• Solution - Ginsenosides

Using the Chromai Leaps high performance

liquid chromatography system to determine the
content of ginseng red ginseng and American
ginseng saponins in water can obtain better
resolution and less baseline noise, and the
results are accurate and reliable.

Instrument: Leaps series ultra- high performance liquid chromatography system

Column temperature: 25
Column: Chromai C18 5µm x 4.6 x 250mm
°C
Mobile phase: A methanol, B water; (gradient elution) Flow rate: 1.0mL/min
Detection wavelength: 203 nm Injection volume: 10μL
Typical Application – Determination of PAHs in
Water
Polycyclic aromatic hydrocarbons are mainly produced
• Solution - PAHs
by the incomplete combustion of organic matter at
high temperature, widely exist in the natural
[mAU]
environment, and have carcinogenic, teratogenic and
mutagenic properties. Hundreds of polycyclic
150
aromatic hydrocarbons have been identified, 16 of
which have been included in the list of priority
6

100
pollutants by the US Environmental Protection Agency
Abs

1 2

( EPA ). In China's latest drinking water standards,

5

50
4

benzo [a] pyrene is listed as a detection component

11
10
3

12

13

16
15
14
and limits are specified.
8

0
0 5 10 15 20 25 30

Time [min.]
Using Chromai Leaps high performance liquid
chromatography system to determine 16 polycyclic
aromatic hydrocarbons in water can obtain better

Instrument: Leaps series ultra- high performance liquid chromatography system resolution and less baseline noise, and the results are
accurate and reliable.
Column: Chromai PAH, 5µm x 4.6 x 250mm Column temperature: 30°C
Mobile phase: A methanol, B water; (gradient elution) Flow rate: 1.2mL/min
Detection wavelength: 254nm Injection volume: 10μL
Typical application – Determination of 18 amino acids in
hydrolyzate such as feed / milk / meat products
Using the Leaps high-performance liquid phase
• Solution - amino acid
system for PITC pre-column derivatization
method to test the amino acid composition, the
detection speed is fast and the method is
sensitive; the derivation is convenient, the
derivative is single and stable , and the detection
result is accurate. Ultraviolet detection ( 254mm )
has high sensitivity, and both primary and
secondary amino acids can be detected.

The method is accurate and reliable for the

determination of amino acid content in feed, milk
and meat products. Moreover, the instrument has
Instrument: Leaps series ultra- high performance liquid chromatography system
a stable baseline and low noise, fully meeting the
Column temperature:
Chromatographic column: Chromai C18 , 5µm x 4.6 x 250mm analysis requirements.
30°C
Mobile phase: phosphate buffer solution (2.5
Flow rate: 1.0mL/min
gKH2PO4+2.5gK2HPO4 dissolved in 1 L water) / methanol
Detection wavelength: 254nm Injection volume: 10μL
Typical Application – Determination of 4
Phthalates in Beverages
• Solution - plasticizer

[mAu]

Plasticizers refer to industrial plastic softeners,

30
mainly bis( 2- ethylhexyl) phthalate ( DEHP ),
dibutyl phthalate ( DBP ), dioctyl phthalate

DEHP
20

DNOP
DBP
DEP

( DNOP ) and diethyl phthalate ( DEP ), etc.

Abs

10
Unscrupulous merchants add plasticizers to foods
instead of palm oil in clouding agents in order to
0
increase the viscosity and stability of beverage

-10 flow. Studies have shown that phthalates play a

0 5 10 15 20 25 30
Time
[min.] role similar to estrogen in humans and animals,
can interfere with endocrine, and cause adverse
effects on the reproductive system. They are
Instrument: Leaps series ultra- high performance liquid chromatography system endocrine disrupting hormones that are
monitored internationally.
Chromatographic column: Chromai C18 , 5µm x 4.6 x 250mm Column temperature: 30°C

Mobile phase: A methanol, B water; (gradient elution) Flow rate: 1.0mL/min

Detection wavelength: 275nm Injection volume: 20μL

Typical application - determination of
oxytetracycline, tetracycline, chlortetracycline and
doxycycline content
• Solution - Veterinary Antibiotics
Instrument: Leaps series ultra- high performance liquid chromatography system
Chromatographic column: Chromai C18 , 5µm x 4.6 x
250mm
Mobile phase: A methanol, B water; (gradient elution)
Detection wavelength: 360nm
In the process of animal husbandry production, in order
to treat livestock and poultry diseases, they are often
given or injected with various antibiotics. Tetracycline
antibiotics have become the main species of veterinary
antibiotics commonly used in recent years because of
their broad-spectrum antibacterial effect and cheap price.
Tetracycline antibiotics mainly include aureomycin,
oxytetracycline, tetracycline and doxycycline.
In this paper , Chromai Leaps high performance liquid
chromatography system was used to determine the
content of these four antibiotics, and ideal analysis results
were obtained. The method verification experiment shows
that the Chromai Leaps HPLC system fully complies with
the requirements specified in the method, and the data
results are accurate and reproducible.
Column temperature:
30°C
Flow rate: 1.0mL/min
Injection volume: 20μL
Typical Application - Determination of Melamine in
Infant Formula
• The Solution — Food Safe Melamine
Figure 1. Chromatogram of melamine reference substance
2. Infant formula chromatogram
Instrument: Leaps series ultra- high performance liquid chromatography system
Column: ASB C8, 5μm, 4.6mm×250mm
Mobile phase: ion-pair reagent buffer ( 2.10 g citric acid
and 2.16 g sodium octane sulfonate
Detection wavelength : 2 40 nm
According to the national standard of the People's
Republic of China GB/T22388-2008 detection method for
melamine in raw milk and dairy products, the content
determination and methodological verification of
melamine in infant formula milk powder produced by a
certain manufacturer were carried out.
The experimental results show that the Chromai Leaps
HPLC system is used to determine the content of
melamine in infant formula milk powder, which can fully
meet the requirements of the national standard of the
Figure
People's Republic of China, and the method is accurate
and sensitive.
Column temperature:
40°C
Flow rate : 1.0mL / min _
Injection volume: 20μL
Typical Application - Separation and Collection of
Snake Venom (SCX) - Size Exclusion

• Solution - snake venom protein

Chromai Leaps high performance liquid

chromatography system for proteomics
using size exclusion method to separate
and collect snake venom protein (SCX)
can fully meet the requirements of national
standards, and the method is accurate and
sensitive .

Instrument: Leaps series ultra- high performance liquid chromatography system

Column: BioCore SCX , 5 μm, 4.6mm×250mm Column temperature: 30°C

Mobile Phase: A) 20 mM Tris-HCl , pH6.0 B) 300 mM NaCl in
Flow rate : 1.0mL / min _
20 mM Tris-HCl , pH6.0
Detection wavelength : 280nm Injection volume: 10μL
 Typical Application - HPLC Test Method for 10
Preservatives in Cosmetics correlat
Peak
Compound regression ion
numb concentration range
• Solution — cosmetic preservatives Chinese name equation coeffici
er
ent
2- Bromo -
25.230µg/mL-1009.200µg/ r=0.999
1 Y=0.96833X
2nitropropane - 1,3
[mAu]

400
mL 7
- diol
25.430µg/mL-1017.200µg/ r=0.999
300 2 Benzyl alcohol Y=1.48417X
mL 8
10.420µg/mL-416.800µg/ r=0.999
Abs

Y=3.56532X
200
3 Phenoxyethanol
mL 9
100
4- Y=113.72681X r=0.999
4 1.004µg/mL-40.160µg/mL
0
Hydroxybenzoate 9
10.060µg/mL-402.400µg/ Y=7.03688X r=0.999
0 10 20 30
[min.]
5 benzoic acid
Time
mL 8

Y=104.14385X r=0.999
6 4- hydroxybenzoate 1.016µg/mL-40.640µg/mL
9
4- Y=95.52889X r=0.999
7 1.032µg/mL-41.280µg/mL
Hydroxybenzoate 9
4- Y=96.93468X r=0.999
8 1.012µg/mL-40.480µg/mL
Hydroxybenzoate 9
4- Y=90.0397X r=0.999
9 2.560µg/mL-102.400µg/mL
Hydroxybenzoate 9
4- r=0.999
10 2.510µg/mL-100.400µg/mL Y=90.12245X
Hydroxybenzoate
9
prehensive advantage

For two fields

General medical clinical
detection clinic medicine

Whole-process analysis and detection based on

chromatographic core technology
Make complex
analysis easier to
use

Automated HPLC Analysis LC-MS /MS Big

Pretreatment Data/web

Analytical testing based on laboratory automation

maintenance

The role of sinker filter head:

( 1 ) Filter the particles that may exist in the mobile phase;
( 2 ) Keep the liquid inlet tube at the bottom of the solvent
bottle.

Sink filter head cleaning method:

( 1 ) Sonicate in 20% methanol solution for 15 minutes;
( 2 ) Sonicate in isopropanol solution for 15 minutes;
( 3 ) Ultrasound in pure methanol solution for 15 minutes;
If it is invalid, it needs to be placed in dilute nitric acid ( 30% ,
volume fraction) for 30 minutes and then cleaned by the above
steps. If it is still ineffective after cleaning, it needs to be
replaced.
maintenance
maintenance

One-way spool Outlet sleeve

Inlet sleeve One-way spool

Sectional High Valve body

view of pressure and spool
pump sealing
head ring
maintenance

A check valve assembly consists of two sets of ruby

balls and sapphire seats. A tight fit between the jewel
ball and the seat is a must for a seal. When the
gemstone ball or the ball seat is polluted, the contact
between the two will not be close enough, which will
Check valve structure
affect the sealing effect. This often happens when diagram
poor-quality organic solvents are used, mobile seal

phases are not filtered, or buffer salts are used. Gemstone/ceramic tee

Gem ball
The check valve is the most fault-prone component Ball seat
in the entire liquid phase system, and developing gasket
good usage habits can effectively avoid this problem.
Valve body
This includes:
( 1 ) Use high-quality organic solvents;
( 2 ) The buffered saline solution should be filtered;
( 3 ) If the buffered salt solution is mixed with an
organic solvent, it will cause the precipitation of salt
particles, so it must be filtered;
( 4 ) Install and disassemble correctly, and clean the
one-way valve.
 maintenance

7725i Manual Injection Valve

 maintenance

7725i Manual Injection Valve

 maintenance

eplacing the Deuterium Lamp

deut
eriu
m
lamp

Schematic diagram of UV detection replacement deuterium lamp

maintenance
The deuterium lamp is a consumable, and after the service life of the deuterium lamp reaches the limit, it will cause problems such as
increased baseline noise and poor linearity. At this time, the user needs to replace the deuterium lamp by himself.
Replacing the Deuterium Lamp
Replacement conditions: The service life of the deuterium lamp is 2000h . When the signal noise increases and the energy decreases
obviously, consider replacing it.
Tools needed : Phillips screwdriver, white cloth gloves

Precautions:
1. Before opening the detector shell, the power cord must be unplugged, and the deuterium lamp must be turned off for more than 15
minutes to avoid burns due to high temperature.
2. Do not touch the glass part of the deuterium lamp directly with your hands to avoid burns and keep the surface of the lamp clean. If the
surface of the lamp is polluted, use gauze dipped in a small amount of isopropyl alcohol to wipe the lamp clean.

Replacement steps:
1. Turn off the detector, unplug the power cord, and ensure that the deuterium lamp is cooled for more than 15 minutes .
2. Use a Phillips screwdriver to remove the screw on the right side cover of the detector, and then remove the right deuterium lamp
protection cover.
3. Unplug the three-pin plug of the old lamp, use a Phillips screwdriver to remove the two fixing screws on the lamp holder of the deuterium
lamp, put on clean white cloth gloves, and take out the entire lamp along the outside direction .
4. Put the new deuterium lamp in the lamp holder, adjust the direction, tighten the screws, and plug in the three-pin plug.
5. Install the light source chamber cover and tighten the screws.
6. Install the shell cover, tighten the screws, and replace the new deuterium lamp , click Replace on the software, the current deuterium lamp
usage time is cleared, and the replacement is completed
Note: It takes 50 hours to reach the best working condition. You can check the working status and energy of the new deuterium lamp through
the workstation .
maintenance

High pressure
infusion pump
Maintenance required by the user:
• cleaning check valve
• Eliminate air bubbles in the pipeline
• Replace vent valve O -ring
• Replacing the Inline Filter Element
• Check the high pressure seal
• Overpressure treatment
• low pressure treatment
maintenance

mixer

Replace in-line filter

maintenance

Detector Maintenance required by the user:

• Replace flow cell
• Clean the flow cell
• Replacing the Deuterium Lamp
• Replacing Tungsten Lamps

Replace flow cell

Replacing Tungsten Lamps
maintenance

whole system Maintenance required by the user:

• system cleaning
• pipeline connection
• replace fuse
• firmware upgrade

system cleaning
The daily cleaning of the instrument should be done well to prevent dust
from affecting the performance of the instrument.
The front panel of the instrument should not be in direct contact with
organic solvents such as acetonitrile and methanol.

pipeline connection
The part of the whole instrument in contact with the liquid is made of
stainless steel, FPE or PEEK , and strong corrosive organic solvents such as
chloroform and tetrahydrofuran cannot be used.
The joints of the instrument pipeline may leak after long-term use or
repeated disassembly and assembly, and users can replace them with the
attached spare parts.
maintenance

Maintenance required by the user:

whole system
• system cleaning
replace fuse • pipeline connection
Parts required: same type of fuse
Required tools: flat-blade screwdriver • replace fuse
Precautions:
1. Before replacing the fuse, turn off the • firmware upgrade
instrument and unplug the power cord to avoid
electric shock.
2. Please use the same type of fuse.

Replacement steps:
1. Turn off the module and unplug the power cord.
2. Use a flat-head screwdriver to pry open the fuse
holder and pull it out.
3. Take out the burnt fuse and install a new one on
the fuse holder.
4. Reinstall the fuse holder and snap it tight.
5. Power on the instrument, if the instrument can
be turned on normally, it proves that the fuse has
been replaced correctly.
maintenance
whole system

• What should I prepare before seeking help ?

Before contacting technical support, users should pay attention to
familiarize themselves with the relevant information of the
problem, including but not limited to:
 Detailed description of the failure phenomenon, the method
used
 probability of failure
 The condition of the instrument (serial number, software and
hardware version, etc. )
 error code (if any)
 Computer screenshots and on-site photos
 Associated Data, Methods and Sequence Files
 Surrounding environmental conditions (temperature, humidity,
power supply, etc.)
No public

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Chromai | Innovation for Better Life