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    Rams Kb
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    The document discusses the autoclave, a pressurized device used to sterilize equipment and materials by heating them above the boiling point of water under increased pressure. It provides a brief history of the autoclave from its development by Papin in the 17th century to its refinement by Pasteur and Chamberland in the late 19th century. The document also outlines how autoclaves work by using pressure to raise the boiling point of water, and describes their common uses in fields like microbiology, medicine, and dentistry.

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    The document discusses the autoclave, a pressurized device used to sterilize equipment and materials by heating them above the boiling point of water under increased pressure. It provides a brief history of the autoclave from its development by Papin in the 17th century to its refinement by Pasteur and Chamberland in the late 19th century. The document also outlines how autoclaves work by using pressure to raise the boiling point of water, and describes their common uses in fields like microbiology, medicine, and dentistry.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as PPT, PDF, TXT or read online from Scribd
    Flag for inappropriate content
    Download as ppt, pdf, or txt
    287 views9 pages

    Autoclave

    Uploaded by

    Rams Kb
    The document discusses the autoclave, a pressurized device used to sterilize equipment and materials by heating them above the boiling point of water under increased pressure. It provides a brief history of the autoclave from its development by Papin in the 17th century to its refinement by Pasteur and Chamberland in the late 19th century. The document also outlines how autoclaves work by using pressure to raise the boiling point of water, and describes their common uses in fields like microbiology, medicine, and dentistry.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as PPT, PDF, TXT or read online from Scribd
    Flag for inappropriate content
    Download as ppt, pdf, or txt
    of 9

    Autoclave

    By: Carolina Gallango-Brun Sana Khan Vedrana Sahovic

    Definition
    Autoclave is a pressurized device designed to heat aqueous solutions above their boiling point at normal atmospheric pressure to achieve sterilization
    Fig. 1. Autoclave machine

    Brief History
    1450 B.C.E. Moses 460-377 B.C.E. Hippocrates Lazzaro Spallanzani Pasteur Denis Papin

    Fig. 2. Papins steam cooker

    20th Century
    It was finalized by Charles Chamberland in 1987 Colleague of Pasteur Building up on Papins marmite From culinary to medicine
    Fig. 3. Charles Chamberland 1851-1908

    How Des it Works?


    Liquid H2O cannot be heated above 100C in an open vessel At 100C boils H2O heated in a sealed vessel Pressure rises Boiling point of H2O is raised

    The Process
    Air removal Downward displacement Steam pulsing Vacuums pumps Superatmospheric Subatmospheric

    Autoclave Usage
    Microbiology Medicine Body piercing Veterinary science Dentistry Podiatry Metallurgy

    Video Clip

    References
    http://en.wikipedia.org/wiki/Autoclave http://138.23.152.128/protocols/autoclave%20operation.pdf

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