1 .

When cleansing the skin with

alcohol and then i o d i n e for the
collection of a blood c u l t u r e r
the i o d i n e (or iodophor)
should remain i n t a c t on the s k i n
for at least:
A ) 1 0 SEcONDS
B ) 3 0 SEcONDS
c ) 6 0 SEcONDS
D ) 5 MINUTES
2 . C l a s s i c approach to b a c t e r i a l i d e n t i f i c a t i o n r and
MOST IDENTIFICATION s t r a t e g i e s are s t i l l based on
bacterial

A)GENOTYPE
B)PhENOTYPE
c)BOTh OF ThESE
D)NONE OF ThESE
3 . The lowest concentration o f a n t i b i o t i c th a t
i n h i b i t s growth o f a t e s t organism i s t h e :

A)MINIMUM I N h I B I T O RY cONcENTRATION
B)SERUM I N h I B I T O RY cONcENTRATION
c)MINIMUM BAcTERIcIDAL T I T E R
D)MAxIMUM I N h I B I T O RY T I T E R
4. Quality control r e s u l t s for disk diffusion
s u s c e p t i b i l i t y t e s t s y i e l d the following r e s u l t s :
aminoglycoside zones too s m a l l and p e n i c i l l i n zones
too l a r g e . T h i s i s most l i k e l y due to t h e :

A)INOcULUM BEINg TOO hEAvY

B)INOcULUM BEINg TOO L I g h T
c)PH OF MUELLER- AgAR BEINg
HINTON TOO LOw
D)CALcIUM AND MAgNESIUM
cONcENTRATION IN ThE AgAR
BEINg
TOO hIgh
ANTIMICROBIAL SUSCEPTIBILITY TEST
DI L UT I O N TESTS

BROTH DI L UTI O N M I c/ M BC 5 X 1 0 ^ 5 CFU/ML

ANAEROBES & N . 1 . 0 X 1 0 ^ 4 CFU/ML

AGAR DILUTION
GONORRHOEAE

DI SK DIFFUSION

AgAR DEPTH: 4 MM ( 3 - 5 MM)

PH: 7 . 2 - 7 . 4
I N c U B A T I O N : 1 8 - 2 4 H AT 3 5 c
STANDARD: 0 . 5 McFARLAND ( 9 9 . 5 ML 1% h2SO4
MUELLER HINTON AGAR AND 0 . 5 ML 1 . 1 7 5 % BAc L 2 )
INOcULUM: 1 . 5 x 1 0 ^ 8 cFU/ML
V E R I F I E D US I Ng SPEcTROPhOTOMETER ( 0 . 0 8 - 0 . 1
AT 6 2 5 NM)
A N T I B I O T I c D I S c : 6 MM
MUELLER-HINTON AgAR USED FOR D I S k DIFFUSION I S STANDARDIzED AT PH 7 . 2 TO 7 . 4 .

DEcREASED A c T I V I T y OF AM I NO gLy c O SI DES , ERyTHROMycIN, c L I N D A M y c I N

LOw Ph
INcREASED A c T I V I T y OF TETRAc yc LI NE

H I g h Ph DEcREASED A c T I V I T y OF TETRAc yc LI NE

ThYMIDINE INcREASED = FALSE ( R ) TO SULFONAMIDES AND TRIMETHOPHRIM

(M I NI M AL/ ABSENT)
CALcIUM ( 2 5 M g / L ) INcREASED = DEcREASED A c T I V I T y OF AMINOgLycOSIDES TO P . AERUgINOSA AND
MAgNESIUM ( 1 2 . 5 M g / L ) DEcREASED A c T I V I T y TO T E T R A c y c L I N E AgAI NST ALL ORgANISM
MUELLER-HINTON AgAR USED FOR D I S k DIFFUSION I S STANDARDIzED AT PH 7 . 2 TO 7 . 4 .

BETTER I N AN A c I D I c ENVIRONMENT, SO zONE S I z E S WOULD BEcOME

LARgER I D THE MEDIA PH I S TOO LOW.
ARE LESS E F F E c T I V E I N AN A c I D I c ENVIRONMENT, SO zONE S I z E S WOULD
BEcOME SMALLER I F THE PH OF THE MEDIA I S TOO LOW.
FALSE ( S ) LARgE zONE

FALSE ( R ) SMALL zONE

FALSE ( R ) , MOISTURE ENHANcES gROWTH

FALSE ( S ) , POOR gROWTH

I NDI c ATO R OF IMPROPER STORAgE

5 . A f t e r s a t i s f a c t o r y performance o f d a i l y d i s k
diffusion s us cep tib ility quality control i s
documentedr the frequency o f q u a l i t y c o n t r o l can be
reduced t o :

A ) Tw I c E A wEEk
B)EvERY wEEk
c)EvERY OThER wEEk
D)EvERY MONTh
6 . Green o r brown zone around the c o l o n i e s :

A)ALPhA hEMOLYSIS
B)BETA hEMOLYSIS
c)GAMMA hEMOLYSIS
D)ALPhA PRIME hEMOLYSIS
A) BETA hEMOLYSIS – NO
hEMOLYSIS
B) GAMMA hEMOLYSIS –
PARTIAL hEMOLYSIS
c ) ALPhA hEMOLYSIS –
cOMPLETE cLEARINg
D ) NONE
7 . Ly s os ta p h i n i s used to d i f f e r e n t i a t e
Staphylococcus from which other genus?

A)STREPTOcOccUS
B)STOMATOcOccUS
c)MIcROcOccUS
D)PLANOcOccUS
 STAPHYLOCOCCUS MICROCOCCUS

GLUCOSE U T I L I Z AT I O N FERMENTER OXIDIZER

MODIFIED OXIDASE - +

AEROBIC GROWTH + +

ANAEROBIC GROWTH + -

LYOSTAPHIN/FURAZOLIDONE S R

BACITRACIN R S

GRAM ( + ) COCCI I N CLUSTERS GRAM ( + ) COCCI I N TETRADS

“GRAPELIKE COCCI” OR SARCINAE
BAP CREAMY WHITE PINHEAD “CLUBLIKE”
COLONIES W/ HEMOLYTIC
PATTERN
8 . A wound ( s k i n l e s i o n ) specimen obtained from a
newborn grew predominantly β - hemolytic colonies
o f gram-p o s i t i v e cocci on 5% sheep
blood a g a r. The newborn i n f a n t was covered
with s m a l l s k i n e r u p t i o n s th a t gave the
appearance o f a “ s c a l d i n g o f the s k i n . ” The gram-
p o s i t i v e c o c c i proved t o be c a t a l a s e p o s i t i v e . Which
t e s t s s h ou ld f o l l o w f o r the a p p rop ri a te
identification?
A ) O P TO c h I N , B I L E S O L U B I L I T Y,
B)COAgULASE, PYR gLUcOSE
DNASE FERMENTATION,
c ) B A c I T R A c I N , PYR , 6 . 5 % SALT BROTh
D)CAMP, B I L E - E S c U L I N , 6 . 5 % SALT
BROTh
8 . A wound ( s k i n l e s i o n ) specimen obtained from a
newborn grew predominantly β - hemolytic colonies
o f gram-p o s i t i v e cocci on 5% sheep
blood a g a r. The newborn i n f a n t was covered
with s m a l l s k i n e r u p t i o n s th a t gave the
appearance o f a “ s c a l d i n g o f the s k i n . ” The gram-
p o s i t i v e c o c c i proved t o be c a t a l a s e p o s i t i v e . Which
t e s t s s h ou ld f o l l o w f o r the a p p rop ri a te
identification?
A ) O P TO c h I N , B I L E S O L U B I L I T Y,
B)COAgULASE, PYR gLUcOSE
DNASE FERMENTATION,
c ) B A c I T R A c I N , PYR , 6 . 5 % SALT BROTh
D)CAMP, B I L E - E S c U L I N , 6 . 5 % SALT
BROTh
8 . A wound ( s k i n l e s i o n ) specimen obtained from a
newborn grew predominantly β - hemolytic colonies
o f gram-p o s i t i v e cocci on 5% sheep
blood a g a r. The newborn i n f a n t was covered
with s m a l l s k i n e r u p t i o n s th a t gave the
appearance o f a “ s c a l d i n g o f the s k i n . ” The gram-
p o s i t i v e c o c c i proved t o be c a t a l a s e p o s i t i v e . Which
t e s t s s h ou ld f o l l o w f o r the a p p rop ri a te
identification?
A ) O P TO c h I N , B I L E S O L U B I L I T Y,
B)COAgULASE, PYR gLUcOSE
DNASE FERMENTATION,
c ) B A c I T R A c I N , PYR , 6 . 5 % SALT BROTh
D)CAMP, B I L E - E S c U L I N , 6 . 5 % SALT
BROTh
9 . An outbreak o f S . aureus i n the n u r s e r y department
prompted the I n f e c t i o n C on trol Committee to proceed
to an environmental s cre e ni n g procedure. The best
s c re e n i n g media to use f o r t h i s purpose would be:

A)CNA AgAR
B ) T H I O AgAR
c)MANNITOL SALT AgAR
D)PEA AgAR
 S E L E c T I V E I SO L AT I O N OF ALL gRAM P O S I T I V E c O c c I , AEROBIc gRAM
PhENYLEThYL ALcOhOL AgAR
P O S I T I V E B A c I L L I , AND ANAEROBIc gRAM NEgATIVE B A c I L L I

COLUMBIA c O L I S T I N N A L I D I x I c
SEL E cT I V E I SO L AT I O N OF gRAM ( + ) c O c c I
A c I D AgAR

SEL E cT I V E I SO L AT I O N OF STAPHyLOcOccI
SUgAR: MANNITOL
MANNITOL SALT AgAR
I N D I c A T O R : PHENOL RED
I N H I B I T O R : 7 . 5 % NAcL (HALOTOLERANT)
THIOGLYCOLATE BROTH ANAEROBES, AEROBES, MICROAEROPHILIC, FASTIDIOUS ORGANISM
 SSA
MAc
NEUTRAL RED
ASHDOWN
cIN

MSA
XLD
PhENOL RED CTA
UREASE
TSI

CITRATE
HEA
BROMThYMOL BLUE OF HUgH LEIFSON
TcBS
ScA

BROMcRESOL PURPLE LIA

10 . B a c i t r a c i n A d i s k s ( 0 . 0 4 u n i t ) are used f o r the
presumptive i d e n t i f i c a t i o n o f which group o f β-
hemolytic s t r e p t o c o c c i ?

A)GROUP
A
B)GROUP
B
c)GROUP
C
D)GROUP F
11 . An i s o l a t e recovered from a v a g i n a l c u l t u r e
obtained from a 2 5 - y e a r - old female p a t i e n t who i s 8
months pregnant i s shown to be a gram-p o s i t i v e c o c c i r
c a t a l a s e n e g a t i v e r and β - hemolytic on blood a g a r.
Which t e s t s are needed f o r f u r t h e r i d e n t i f i c a t i o n ?

A ) O P TO c h I N , B I L E S O L U B I L I T Y,
PYR B ) B A c I T R A c I N ,
CAMP, PYR
c)METhIcILLIN, PYR , TREhALOSE
D)COAgULASE, gLUcOSE, PYR
8 . A wound ( s k i n l e s i o n ) specimen obtained from a
newborn grew predominantly β - hemolytic colonies
o f gram-p o s i t i v e cocci on 5% sheep
blood a g a r. The newborn i n f a n t was covered
with s m a l l s k i n e r u p t i o n s th a t gave the
appearance o f a “ s c a l d i n g o f the s k i n . ” The gram-
p o s i t i v e c o c c i proved t o be c a t a l a s e p o s i t i v e . Which
t e s t s s h ou ld f o l l o w f o r the a p p rop ri a te
identification?
A ) O P TO c h I N , B I L E S O L U B I L I T Y,
B)COAgULASE, PYR gLUcOSE
DNASE FERMENTATION,
c ) B A c I T R A c I N , PYR , 6 . 5 % SALT BROTh
D)CAMP, B I L E - E S c U L I N , 6 . 5 % SALT
BROTh
12 . A sputum specimen from an 8 9 - y e a r - old male
p a t i e n t with suspected b a c t e r i a l pneumonia grew a
predominance o f gram-p o s i t i v e c o c c i d i s p l a y i n g alpha
hemolysis on 5% sheep blood a g a r. The c o l o n i e s
appeared donut shaped and mucoidy and t e s t e d negative
f o r c a t a l a s e . The most a p p rop r i a te t e s t s f o r a f i n a l
identification are:

A)COAgULASE, gLUcOSE FERMENTATION,

LYSOSTAPhIN
B)PENIcILLIN, BAcITRAcIN, CAMP
c)OPTOchIN, B I L E S O L U B I L I T Y, PYR
D)BILE EScULIN,
hIPPURATE hYDROLYSIS
8 . A wound ( s k i n l e s i o n ) specimen obtained from a
newborn grew predominantly β - hemolytic colonies
o f gram-p o s i t i v e cocci on 5% sheep
blood a g a r. The newborn i n f a n t was covered
with s m a l l s k i n e r u p t i o n s th a t gave the
appearance o f a “ s c a l d i n g o f the s k i n . ” The gram-
p o s i t i v e c o c c i proved t o be c a t a l a s e p o s i t i v e . Which
t e s t s s h ou ld f o l l o w f o r the a p p rop ri a te
identification?
A ) O P TO c h I N , B I L E S O L U B I L I T Y,
B)COAgULASE, PYR gLUcOSE
DNASE FERMENTATION,
c ) B A c I T R A c I N , PYR , 6 . 5 % SALT BROTh
D)CAMP, B I L E - E S c U L I N , 6 . 5 % SALT
BROTh
8 . A wound ( s k i n l e s i o n ) specimen obtained from a
newborn grew predominantly β - hemolytic colonies
o f gram-p o s i t i v e cocci on 5% sheep
blood a g a r. The newborn i n f a n t was covered
with s m a l l s k i n e r u p t i o n s th a t gave the
appearance o f a “ s c a l d i n g o f the s k i n . ” The gram-
p o s i t i v e c o c c i proved t o be c a t a l a s e p o s i t i v e . Which
t e s t s s h ou ld f o l l o w f o r the a p p rop ri a te
identification?
A ) O P TO c h I N , B I L E S O L U B I L I T Y,
B)COAgULASE, PYR gLUcOSE
DNASE FERMENTATION,
c ) B A c I T R A c I N , PYR , 6 . 5 % SALT BROTh
D)CAMP, B I L E - E S c U L I N , 6 . 5 % SALT
BROTh
13 . The Superoxol t e s t i s used a s a r a p i d presumptive
test for:

A ) N EIS S E R IA gONORRhOEAE
B ) N EIS S E R IA M E N I N g I T I D I S
c ) N E I S S E R I A LAcTAMIcA
D)MORAxELLA cATARRhALIS
14 . A sputum c u l t u r e from an a l c o h o l i c seen i n the ER
grows g r a y r mucoidr s t r i n g y c o l o n i e s on sheep blood
a g a r. The i s o l a t e grows r e a d i l y on MacConkey agar and
forms mucoidr dark p ink c o l o n i e s . The c o l o n i e s y i e l d
the f o l l o w i n g t e s t r e s u l t s :

ONPG +
Indole –
A)EDwARDSIELLA TARDA

Glucose + B)KLEBSIELLA PNEUMONIAE

Oxidase – c)ESchERIchIA cOLI
Citrate + D)PROTEUS vULgARIS
VP +
15 . The f o l l o w i n g r e s u l t s were obtained from a pure
c u l t u r e o f gram-negative rod s recovered from the
pulmonary s e c r e t i o n s o f a 1 0 - y e a r - old c y s t i c
f i b r o s i s patient with pneumonia:

O x idas e : p o s i t i v e
A) BURkhOLDERIA PSEUDOMALLEI
Glucose OF ( o p e n ) : p o s i t i v e
B) PSEUDOMONAS STUTzERI
Pigment: red ( n o n f l u o r e s c e n t )
c ) BURkhOLDERIA cEPAcIA
Growth a t 42°C: p o s i t i v e
D ) PSEUDOMONAS AERUgINOSA
Motility: positive
Gelatin hydrolysis: positive
Arginine dihydrolase: positive
F l a g e l l a : p o l a r r monotrichous
OF TEST
• A B I L I T Y TO PRODUcE A c I D END PRODUcTS FROM SUBSTRATE U T I L I z AT I O N I N ThE
PRESENcE/ABSENcE OF OxYgEN
• OF MEDIUM w I T h gLUcOSE – MEDIUM

• MINERAL O I L – OxYgEN BARRIER

• 2 OF TUBES ARE U T I L I z E D , ONE OvERLAID w I T h MINERAL O I L
• ANDRADE’S A c I D F U c h S I N , BROMcRESOL PURPLE, BROMThYMOL BLUE, PhENOL RED – PH
INDIcATOR
• HUgh AND LEIFSON MEDIUM – OF MEDIUM w I T h 1% CHO AND BROMThYMOL BLUE AS PH
INDIcATOR

OPEN TUBE CLOSED TUBE

FERMENTER YELLOW yELLOW
OxIDIzER yELLOW BLUE/gREEN
16 . A c u l t u r e from an i n f e c t e d dog b i t e on a s m a l l
b o y ’s f i n g e r y i e l d e d a s m a l l r gram-negative
c o c c o b a c i l l u s th a t was smoothr r a i s e d and beta-
hemolytic on blood a g a r. The i s o l a t e grew on
MacConkey a g a r r forming c o l o r l e s s c o l o n i e s . The
organism was m o t i l e r c a t a l a s e p o s i t i v e r oxidase
p o s i t i v e r reduced n i t r a t e r and was urease p o s i t i v e
w i t h i n 4 h o u r s . No carbohydrates were fermented. The
most l i k e l y i d e n t i f i c a t i o n o f t h i s i s o l a t e i s :

A)BRUcELLA cANIS
B ) Y E R S I N I A PESTIS
c)FRANcISELLA TULARENSIS
D)BORDETELLA BRONchISEPTIcA
 UREASE M O TI LI TY NITRATE OxIDASE

- - - +
B . PERTUSSIS

+ - - -
B . PARAPERTUSSIS

+ + + +
B . BRONchISEPTIcA

UREASE

-
ALcALIgENES

+
B . BRONchISEPTIcA
 17.A sputum specimen i s received f o r
c u l t u r e and Gram s t a i n . Thespecimen
contains 15 e p i t h e l i a l
c e l l s / L P F. The t e c h n o l o g i s t ’s best course o f
action
would be t o :

A) INOcULATE APPROPRIATE
MEDIA AND INcUBATE
ANAEROBIcALLY
B) INOcULATE APPROPRIATE
MEDIA AND
INcUBATE AEROBIcALLY
c) C A LL ThE P h Y S I c I A N AND
NOTIFY h I M OF ThIS LIFE
ThREATENINg SITUATION
D)CALL ThE PATIENT cARE
AREA AND
 • ASSESS THE Q UA L I T y OF SPUTUM
SAMPLES
• I T I S REcOMMENDED THAT ALL
SPEcIMENS c O N T A I N I N g PURULENT
SEcRETIONS SHOULD BE cULTURED,
WHEREAS THOSE c O N T A I N I N g ORAL
BARTLETT’S c L A S S I F I c AT I O N SEcRETIONS SHOULD BE
REcOLLEcTED.

• AccEPTABLE SPUTUM:
• LESS THAN 1 0 E P I T H E L I A L cELLS
/LPF
• GREATER THAN 2 5 PMNS / L P F
18 . Propionibacterium acnes i s most often a s s o c i a t e d
with:

A)NORMAL ORAL FLORA

B ) P O S T- A N T I B I O T I c DIARRhEA
c)TOOTh DEcAY
D)BLOOD cULTURE cONTAMINATION
 MOST cOMMON cAUSE OF DEATH B y BAc TE RI AL cONTAMINATED
Y . ENTEROcOLITIcA BLOOD BAgS ( c D c )
MOST cOMMON ISOLATE FOUND I N RBc UNITS

PSEUDOMONAS 2 N D MOST cOMMON ISOLATE I N RBc UNITS

P. AcNES MOST cOMMON BAc TE RI AL cONTAMINANTS I N RBc

MOST FREQUENTLy REcOVERED FROM DONATED BLOOD AND

B . cEREUS, S . EPIDERMIDIS
cONTAMINATION OF PLATELET
GREATEST NUMBER OF DEATH, MOST cOMMON BAcTERIAL
S . AUREUS
cONTAMINANT (HARMENINg)
19 . Pa r a s i t i c organisms th a t are most often
transm itted s e x u a l l y i n c l u d e :

A)ENTAMOEBA gINgIvALIS
B)DIENTAMOEBA F R A g I L I S
c)TRIchOMONAS v A g I N A L I S
D)DIPhYLLOBOThRIUM LATUM
20 . L a r g e r r u s u a l l y e c c e n t r i c r e f r a c t i l e g r a n u l e s may
be on one s i d e o f karyosome (“bas ket n u c l e u s ” ) :

A) E . h I S TO LY T I c A
B) E . c O L I
c ) E . NANA
D)I. BUTSchLII
2 1 . One o f the f o l l o w i n g protozoan organisms has been
i m p l i c a t e d i n waterborne and foodborne ou tb r e a k s . The
s u s p e c t organism i s :

A)PENTATRIchOMONAS hOMINIS
B)DIENTAMOEBA F R A g I L I S
c ) G I A R D I A LAMBLIA
D)BALANTIDIUM c O L I
22 . The s t r i n g t e s t f o r o b t a i n i n g a s m a l l specimen o f
the duodenal contents f o r p a r a s i t i c i n f e c t i o n would
be most ap p ropriate f o r

A) G . LAMBLIA
B) C . M E S N I L I
c ) E . NANA
D ) E . h I S TO LY T I c A
 • GELATIN cAPSULE WITH c O I L ED
yARN
ENTERIc • AFTER 4 HOURS, yARN I S
RETRIEVED AND MUcOUS
cAPSULE I S ExAMINED
TE ST/S TRINg • FOR g . L A M B L I A , S .
TEST STERcO RALI S, cRyPTOSPORIDIUM
S P P , ISOSPORA B E L L I , EggS
OF
F . HEPATI cA AND c . SI NENS I S
23 . Eye i n f e c t i o n with Acanthamoeba spp have most
commonly been traced to

A)USE OF SOFT cONTAcT LENS

B)USE OF hARD cONTAcT LENS
c ) U S E OF cONTAMINATED cONTAcT LENS
SOLUTION
D)FAILURE TO REMOvE cONTAcT LENS
whILE SwIMMINg
24 . A 12year o l d g i r l i s brought to the
emergency department with meningitis and a
h i s t o r y o f swimming i n a warm water s p r i n g .
10 umM oitni l es iamoebae th a t measure
z e are seen i n the CSF and are most l i k e l y

A) I . BUTSchLII
B) D . TROPhOzOITES F R A g I L I S
c)E. TROPhOzOITES NANA
D)N. TROPhOzOITES
FOwLERI TROPhOzOITE
 • PRIMARY AMEBIc MENINgOENcEPhALITIS
( PAM )
• BRAIN E A T I N g AMOEBA
N. FOwLERI • D I A g N O S I S : c SF ExAMINATION
• MOT: I N H A L A T I O N , S N I F F I N g , SWIMMINg
I N cONTAMINATED WATER
• KERnig’s sign

• N. FOWLERI/AcANTHAMOEBA SPP – NON

NUTRIENT AgAR WITH E . c O L I
cSF • TRyPANOSOMA – AFRI cAN SLEEPINg
SIcKNESS
• SHOULD BE DONE W I T HI N 2 0 MINS
25 . A 45year o l d malesheepherder i n California
p re s e n ts with h e p a t i t i s . S i n c e other c r i t e r i a are not
metr X - r a y angiography i s performed o f the abdomen
with the r e s u l t th a t a tumor l i k e body i s revealed
w i t h i n the l i v e r. Because o f h i s occup a ti onr a c a s o n i
t e s t i s performed and found p o s i t i v e . S u r g i c a l
removal o f the tumor r e v e a l s a l a r g e c y s t f u l l o f
h y d a ti d s a n d . What i s the most l i k e l y e t i o l o g y agent
involved i n t h i s infection?

A) D . LATUM
B) H . NANA
c ) F. hEPATIcA
D ) E . gRANULOSUS
 • E . gRANULOSUS
• A N T I g E N : STERI LE HyDATID
F L UI D
• S E N S I T I V E PxN = LARgE whEAL
CASONI TEST ( 5 cM OR MORE) w I T h
FORMATION OF PSEUDOPODIA
w I T h I N 3 0 MINS w I T h NO
REAcTION I N cONTROL ARM
26 . A primary d i f f e r e n t i a l fe a ture between an H. nana
egg and H. diminuta egg i s which o f the f o l l o w i n g ?

A)A FLATTENED SIDE OF H . DIMINUTA

Egg
B)POLAR FILAMENTS I N H . NANA Egg
c ) T h I c k ShELL FOR H . NANA Egg
D)RADIA STRIATION I N H . DIMINUTA
L
Egg
27 . S k i n s n i p s are the specimen o f choice f o r
d i a g n o s i s o f i n f e c t i o n with

A) L . LOA
B) O . vOLvULUS
c ) B . MALAYI
D ) W. BANcROFTI
 DEScRIPTION
PERIODIcITy
( N Uc E US )

NOT ExTENDINg TO T I P
W. BANcROFTI
OF T A I L

B . MA L A yI 2 TERMINAL T A I L

ExTENDINg TO T I P OF
L . LOA TAIL
NOT ExTENDINg TO T I P
O . VOLVOLUS OF THE T A I L
NOT ExTENDINg TO T I P
M . OzzARDI OF THE T A I L
ExTENDINg TO T I P OF
M . PERSTANS TAIL
M. ExTENDINg TO T I P OF
STREPTOcERcA T A I L (cURVED)
28 . Technique to b est diagnose m i c r o f i l a r i a while
s t i l l v i s i b l e i n the p e r i p h e r a l blood

A ) A c I D EThER cONcENTRATION
B)ZNSO4 cONcENTRATION TEchNIQUE
c ) K N O T T ’ S TEchNIQUE
D)KOH
29 . The mouth o f Necator americanus i s ch a ra cte r i ze d
by the presence o f

A)TEETh
B)CUTTINg PLATES
c)BOTh
D)NEIThER
30 . E n t e r i c cy top a th i c human orphan because the
d i s e a s e a s s o c i a t e d with these agents was not
i n i t i a l l y known. S i n c e 1967r newly i s o l a t e d
e n t e r o v i r u s e s have been d i s t i n g u i s h e d numerically

A)COxSAckIEvIRUS
B)EchOvIRUS
c)POLIOvIRUS
D ) H E PAT I T I S A vIR U S
31 . Owl’s eye c y t o l o g i c a l i n c l u s i o n s are
c h a r a c t e r i s t i c o f i n f e c t i o n caused by

A)HIV
B)CMV
c)HSV
D)VZV
 NEgRI BODIES
TORRES
cOUNcILMAN
BODIES
COwDRY TYPE A
OwL EYES
KOILOcYTOSIS
HENDERSON
PATTERSON
BODIES
GUARNERI BODIES
32 . Which o f the f o l l o w i n g swabs cannot be used f o r
specimens intended f o r v i r a l t e s t i n g ?

A)COTTON SwAB
B)DAcRON SwABS
c) C A LcIU M ALgINATE SwABS
D)RAYON
SwABS
33 . T. rubrum and T. mentagrophytes may be
d i f f e r e n t i a t e d by

A)CONSISTENTLY DIFFERENT
APPEARANcE OF ThEIR
cOLONIES B)ENDOThRIx
hA IR I N F E c T I O N
PRODUcED BY T.
RUBRUM
c)FLUOREScENcE OF hAIRS
INFEcTED w I T h T. RUBRUM
D)IN vITRO PENETRATION BY T .
hA IR
MENTAgROPhYTES
 HAIR
RED
UREASE BAITING
PIGMENT
TEST

T.
RUBRUM
T. MENTAGROPHYTES
34 . A tube o f s e m i s o l i d medium th a t c o n t a i n s
r e s a z u r i n appears p i n k . What does t h i s i n d i c a t e ?

A ) A c I D ENvIRONMENT
B)PRESENcE OF
c ) A L k A L I N E OxYgEN
D ) M O T I L I T Y ENvIRONMENT
35 . I n the N-a c e t y l - L - c y s t e i n e - a l k a l i method o f
p r o c e s s i n g sputum specimens f o r mycobacterial
c u l t u r e r the NALC s e r v e s a s

A)BUFFER
B)DEcONTAMINANT
c)DIgESTANT
D)PH S TA B I L I z E R
36 . Which two t e s t s can be used to d i f f e r e n t i a t e C.
d i p h t h e r i a e from other cory n e b a cte r i a ?

A)HALO ON TINSDALE AND NITRATE

TEST
B)HALO ON TINSDALE AND UREA
hYDROLYSIS
c ) B L A c k ON TINSDALE AND EScULIN
TEST
D)BLAck ON TINSDALE AND NITRATE
TEST
37 . A f a s t i d i o u s gram negative b a c i l l u s was i s o l a t e d
from a case o f p e r i o d o n t a l d i s e a s e r which upon
d a r k f i e l d examination was noted to have g l i d i n g
m o t i l i t y. The most l i k e l y i d e n t i f i c a t i o n o f t h i s
e t i o l o g i c agent would be

A)CAPNOcYTOPhAgA
B)PASTEURELLA
c)PROTEUS
D)PSEUDOMONAS
 RAPID
DARTINg/ShOOTINg
STAR MOTILITY

DARTINg MOTILITY
SwARMINg
TUMBLINg
GLIDINg
cORkScREw
38 . Which group o f t e s t best d i f f e r e n t i a t e
Helicob acter p y l o r i from Campylobacter j e j u n i ?

A)CATALASE, O x I D A S E , gRAM STAIN

B)CATALASE, O x ID A S E , N A L I D I x I c
AcID SENSITIvITY
c)CATALASE, O x I D A S E , cEPhALOThIN
SENSITIvITY
D)UREASE, N I T R AT E , HIPPURATE
hYDROLYSIS
39 . The e n te rotoxi n o f both V. cholerae O1 and
t o x i g e n i c s t r a i n s o f E . c o l i produce s e r i o u s d i a r r h e a
by what mechanism?

A)STIMULATION OF ADENYLATE cYcLASE

whIch g I v E S RISE TO FLU ID
SEcRETION cELLS OF ThE
BY IN TESTIN ES SMALL
B)PENETRATION
c)STIMULATION OF ThE BOwEL
PRODUcTION MUcOSA

D)ELEvATION OF c O L I c I N
OFDERMONEcROTIzINg TOxIN
ThE
40 . He i s sometimes r e f e r r e d to a s the f a t h e r o f
m i cr ob i ol og y r f a t h e r o f b a c t e r i o l o g y r and the f a t h e r
o f protozoology

A)HOOkE
B)LEEwENhOEk
c)PASTEUR
D)KOch
41 . Referred to a s the f a t h e r o f hand washingr the
f a t h e r o f hand d i s i n f e c t i o n r and the f a t h e r o f
h o s p i t a l epidemiology

A)LISTER
B)SEMMELwEIS
c)EhRLIch
D)JENNER
 42.The blue area o f the National
F i r e Pr o t e c t i o n A s s o c i a t i o n hazard rating
indicates

A) R E A c T I v I T Y
B)S P E c I F I c
hAzARD c)HEALTh
D)FLAMMABILITY
43 . The b est way to prevent HIV i n f e c t i o n when
h a n d l i n g blood i s

A)REFUSE TO PROcESS H I V
BLOOD
B)KEEP H I V BLOOD SEPARATE FROM ThE
REST
c)WEAR gLOvES AND wASh AREA w I T h
DILUTE
chLOROx BLOOD AND BODY FLUIDS
D)TREAT ALL
AS POTENTIALLY H I V INFEcTED
43 . The b est way to prevent HIV i n f e c t i o n when
h a n d l i n g blood i s

A)REFUSE TO PROcESS H I V
BLOOD
B)KEEP H I V BLOOD SEPARATE FROM ThE
REST
c)WEAR gLOvES AND wASh AREA w I T h
DILUTE
chLOROx BLOOD AND BODY FLUIDS
D)TREAT ALL
AS POTENTIALLY H I V INFEcTED
44 . Assuming th a t clean catch midstream u r i n e was
processedr which o f the f o l l o w i n g i s i n d i c a t i v e o f
urinary tract infection?

A ) < 1 0 0 0 CFU/ML
B ) > 1 0 0 0 CFU/ML
c ) > 1 0 0 0 0 CFU/ML
D ) > 1 0 0 0 0 0 CFU/ML
45 . Rheumatic f e v e r i s p o s t s t r e p t o c o c c a l sequelae
t y p i c a l l y a s s o c i a t e d with

A)POSTPARTUM IN F E c T IO N S ASSOcIATED
wITh GROUP B
STREPTOcOccI
I N F E c T I O N S , PYODERMAS
B)S kIN
ASSOcIATED wITh
GROUP A
STREPTOcOccI wITh
c ) PGROUP
h A RY N g I TAI S ASSOcIATED
STREPTOcOccI wITh S .
D)PNEUMONIA
ASSOcIATED
PNEUMONIAE
46 . Decarboxylase r e a c t i o n o f E . cloacae

A)LDC +
B ) L D C , ODC +
c)ODC +
D ) O D C , ADC +
 LDC ODC ADH

K . PNEUMONIAE + - -
K . OXYTOCA + - -
E . AEROGENES + + -
E . CLOACAE - + +
P. AGGLOMERANS - - -
P. SHIGELLOIDES + + +
47 . A gram negative rod i s i s o l a t e d from a p a t i e n t
with second and t h i r d degree b u r n s . The i s o l a t e
produces a b l u i s h green pigment and a c h a r a c t e r i s t i c
f r u i t y odor.

A)AcINETOBAcTER
TSI alk/alk
B)ALcALIgENES
OF: O x i d a ti ve u t i l i z a t i o n
c)MORAxELLA
Oxidase +
D)PSEUDOMONAS
Motility +
48 . I t i s defined a s some type o f o b j e c t i v e evidence
o f a d i s e a s e . For exampler while p a l p i t a t i n g a
p a t i e n t r a p h y s i c i a n might d i s c o v e r a lump o r an
enlarged l i v e r o r s p l e e n .

A)SIgN
B)SYMPTOM
c ) I N c U B AT I O N
D)NOTA
49 . Which d i s i n f e c t a n t i n a c t i v a t e s HIV and HBV?

A)ALcOhOL
B)IODINE
c)PhENOL
D)SODIUM hYPOchLORITE
50 . Delayed t e s t f o r CSF

A)REFRIgERATE 4 - 6
B)FROzEN - 2 0 C
C hOURS
c ) R T FOR 2 4 AT
D)INcUBATE 37 C
AND cULTURE ASAP