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  • Matrix Functional Studies - 2024

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    This document outlines different types of functional studies that can be performed at the DNA, RNA, and protein levels in intact cells/tissues or cell/tissue lysates. It lists techniques such as fluorescent in situ hybridization to study spatial expression of DNA and RNA, PCR and sequencing to analyze DNA and RNA, and western blotting and mass spectrometry to analyze proteins. It also provides examples of ways to modify genes and gene expression, including CRISPR genome editing, RNA interference, and transgenic overexpression approaches.

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    Matrix Functional Studies_2024

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    This document outlines different types of functional studies that can be performed at the DNA, RNA, and protein levels in intact cells/tissues or cell/tissue lysates. It lists techniques such as fluorescent in situ hybridization to study spatial expression of DNA and RNA, PCR and sequencing to analyze DNA and RNA, and western blotting and mass spectrometry to analyze proteins. It also provides examples of ways to modify genes and gene expression, including CRISPR genome editing, RNA interference, and transgenic overexpression approaches.

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    © All Rights Reserved
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    3 views1 page

    Matrix Functional Studies - 2024

    Uploaded by

    arnehoushmand9
    This document outlines different types of functional studies that can be performed at the DNA, RNA, and protein levels in intact cells/tissues or cell/tissue lysates. It lists techniques such as fluorescent in situ hybridization to study spatial expression of DNA and RNA, PCR and sequencing to analyze DNA and RNA, and western blotting and mass spectrometry to analyze proteins. It also provides examples of ways to modify genes and gene expression, including CRISPR genome editing, RNA interference, and transgenic overexpression approaches.

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    of 1

    FUNCTIONAL STUDIES

    TYPE DNA (GENE) RNA PROTEIN

    INTACT CELL /TISSUE INTACT CELL /TISSUE INTACT CELL /TISSUE

    (Fluorescent) in situ (Fluorescent) in situ Immunohistochemistry


    hybridization hybridization (IHC)
    Immunocytochemistry
    Spatial genomics Spatial transcriptomics (ICC)

    CELL/TISSUE LYSATE CELL/TISSUE LYSATE CELL/TISSUE LYSATE


    FIND IT PCR Short-read or long-read Western blot
    RNA sequencing (bulk)
    Short-read or long-read
    DNA sequencing Mass-spectrometry
    Northern blot (old)
    (Proteomics,
    Peptidomics)
    RT-(q)PCR
    Southern blot
    Single-cell sequencing
    Epigenetic sequencing
    (methylation, ATACseq,
    HiC, …)

    CRISPR genome editing RNA editing (CRISPR) Antibodies


    (Non Homologous end
    joining, Homology RNAi:dsRNA, siRNA, Receptor antagonists
    directed repair, base esiRNA, shRNA,
    editing) morpholino

    LOSE IT Nanobodies
    Homologous
    recombination in
    embryonic stem cells
    Morpholinos

    Knock-in using CRISPR or Protein transfection in


    CRISPR-activation mRNA injection or cells
    electroporation
    Transgenic
    Protein injection or
    overexpression mRNA transfection or electroporation
    Transient transfection transduction in cells
    MOVE IT Implantation of beads
    Transposases (sleeping soaked in a secreted
    beauty, Tol) factor
    Meganucleases (Sci-I)
    Random integration in
    oocytes

    Knock-in using
    homologous

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